Agromyces sp. CFH 90414

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Agromyces; unclassified Agromyces

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3536 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6I2FFP7|A0A6I2FFP7_9MICO Alpha-mann_mid domain-containing protein OS=Agromyces sp. CFH 90414 OX=2662258 GN=GE115_12760 PE=3 SV=1
MM1 pKa = 7.12SRR3 pKa = 11.84KK4 pKa = 9.16IGRR7 pKa = 11.84IVAASLATLAVAATAGCTSAGSGDD31 pKa = 3.77SGGGTIEE38 pKa = 4.4LWTHH42 pKa = 6.2AGGNEE47 pKa = 3.94AEE49 pKa = 4.59LGVITSMVDD58 pKa = 3.4DD59 pKa = 5.38FNASQDD65 pKa = 3.75DD66 pKa = 4.15YY67 pKa = 11.03TVEE70 pKa = 4.03ITDD73 pKa = 4.57FPQDD77 pKa = 3.26AYY79 pKa = 11.67NDD81 pKa = 3.93AVVAAATSDD90 pKa = 3.77NLPCIVDD97 pKa = 3.02IDD99 pKa = 4.27GPNVANWAWAGYY111 pKa = 8.28IQPLGLPEE119 pKa = 3.99EE120 pKa = 4.73TYY122 pKa = 10.98DD123 pKa = 3.69GQLAGTLGVVDD134 pKa = 4.9DD135 pKa = 4.41EE136 pKa = 5.09VYY138 pKa = 10.56AYY140 pKa = 10.75GFFDD144 pKa = 3.21VALAMFSRR152 pKa = 11.84EE153 pKa = 3.93STLADD158 pKa = 3.15AGVRR162 pKa = 11.84VPTVDD167 pKa = 3.76EE168 pKa = 4.4PWTGEE173 pKa = 3.91EE174 pKa = 4.72FADD177 pKa = 4.2ALAKK181 pKa = 9.93IDD183 pKa = 3.4GLNRR187 pKa = 11.84FDD189 pKa = 3.95YY190 pKa = 11.12AVDD193 pKa = 4.45FGTGGGGEE201 pKa = 4.71WIPYY205 pKa = 9.58AYY207 pKa = 10.4SPLLQSFGGDD217 pKa = 3.31LIDD220 pKa = 5.4RR221 pKa = 11.84SDD223 pKa = 3.52FQTADD228 pKa = 3.19GVLNGPEE235 pKa = 3.65ALAWAEE241 pKa = 4.04WFRR244 pKa = 11.84GLVDD248 pKa = 3.52SGYY251 pKa = 8.61MAQASGEE258 pKa = 4.15DD259 pKa = 3.34ATADD263 pKa = 3.78FVNGTSGILYY273 pKa = 7.9TGNWADD279 pKa = 3.57TTVRR283 pKa = 11.84EE284 pKa = 4.34AFDD287 pKa = 4.16DD288 pKa = 4.38AVVLPPPDD296 pKa = 4.41FGDD299 pKa = 4.06GPKK302 pKa = 9.83IGAGSWQWGLTTGCDD317 pKa = 3.6DD318 pKa = 3.39VDD320 pKa = 3.59GARR323 pKa = 11.84AYY325 pKa = 11.18LEE327 pKa = 4.24FAHH330 pKa = 6.07DD331 pKa = 3.25TKK333 pKa = 11.55YY334 pKa = 10.43FVQYY338 pKa = 11.22GEE340 pKa = 4.15ATGLIPATLDD350 pKa = 3.12AAAEE354 pKa = 4.08IPAYY358 pKa = 10.47AEE360 pKa = 4.38GGDD363 pKa = 3.38NRR365 pKa = 11.84IYY367 pKa = 11.1LEE369 pKa = 4.66LADD372 pKa = 3.6QFAVVRR378 pKa = 11.84PEE380 pKa = 3.7TAAYY384 pKa = 8.6PYY386 pKa = 10.07ISSVFQKK393 pKa = 9.54ATQDD397 pKa = 3.29ILSGGDD403 pKa = 3.1AKK405 pKa = 11.17SILDD409 pKa = 3.98KK410 pKa = 11.03AVSDD414 pKa = 3.72IDD416 pKa = 5.63RR417 pKa = 11.84DD418 pKa = 3.55IQQNGGYY425 pKa = 9.83EE426 pKa = 3.98FF427 pKa = 4.93

Molecular weight: 45.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6I2FFI2|A0A6I2FFI2_9MICO Methylated-DNA--protein-cysteine methyltransferase OS=Agromyces sp. CFH 90414 OX=2662258 GN=GE115_16505 PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3536

0

3536

1176171

29

3702

332.6

35.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.395 ± 0.067

0.465 ± 0.01

6.339 ± 0.046

5.974 ± 0.035

3.132 ± 0.025

9.315 ± 0.045

1.941 ± 0.021

4.203 ± 0.035

1.632 ± 0.028

10.009 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.616 ± 0.018

1.75 ± 0.02

5.628 ± 0.036

2.536 ± 0.021

7.67 ± 0.054

5.237 ± 0.026

5.642 ± 0.041

9.078 ± 0.046

1.532 ± 0.018

1.904 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski