Brevibacillus borstelensis AK1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Brevibacillus; Brevibacillus borstelensis

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4951 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M8DE45|M8DE45_9BACL Transcriptional regulator OS=Brevibacillus borstelensis AK1 OX=1300222 GN=I532_15461 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.97PLVEE6 pKa = 5.18FCASNVSSYY15 pKa = 9.72TKK17 pKa = 10.06PVMEE21 pKa = 4.95ALEE24 pKa = 4.64NDD26 pKa = 3.66PEE28 pKa = 5.44LDD30 pKa = 3.45VDD32 pKa = 3.97VLEE35 pKa = 4.5YY36 pKa = 11.28GCLGYY41 pKa = 10.77CGEE44 pKa = 5.53CYY46 pKa = 8.66MQPFALVNGDD56 pKa = 3.8LVQAEE61 pKa = 4.41TADD64 pKa = 3.73EE65 pKa = 4.14LLEE68 pKa = 4.52KK69 pKa = 10.54IKK71 pKa = 11.07AKK73 pKa = 10.66LKK75 pKa = 10.54ADD77 pKa = 3.7EE78 pKa = 4.82EE79 pKa = 4.34EE80 pKa = 4.7QEE82 pKa = 4.63DD83 pKa = 4.89FPFF86 pKa = 5.26

Molecular weight: 9.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M8E090|M8E090_9BACL Uncharacterized protein OS=Brevibacillus borstelensis AK1 OX=1300222 GN=I532_13654 PE=4 SV=1
MM1 pKa = 7.41KK2 pKa = 10.2PSFNPNNRR10 pKa = 11.84KK11 pKa = 9.37RR12 pKa = 11.84KK13 pKa = 8.62KK14 pKa = 9.81DD15 pKa = 2.98HH16 pKa = 6.23GFRR19 pKa = 11.84KK20 pKa = 10.03RR21 pKa = 11.84MSTKK25 pKa = 10.18NGRR28 pKa = 11.84KK29 pKa = 8.91VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.81GRR39 pKa = 11.84KK40 pKa = 8.75VLSAA44 pKa = 4.05

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4951

0

4951

1481559

27

3509

299.2

33.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.725 ± 0.039

0.78 ± 0.011

4.901 ± 0.025

7.175 ± 0.039

4.056 ± 0.024

7.465 ± 0.032

2.098 ± 0.018

6.342 ± 0.031

5.488 ± 0.035

10.233 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.778 ± 0.019

3.196 ± 0.022

4.253 ± 0.026

4.07 ± 0.025

5.436 ± 0.035

5.799 ± 0.028

5.271 ± 0.028

7.545 ± 0.03

1.194 ± 0.014

3.196 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski