Cetobacterium somerae ATCC BAA-474

Taxonomy: cellular organisms; Bacteria; Fusobacteria; Fusobacteriia; Fusobacteriales; Fusobacteriaceae; Cetobacterium; Cetobacterium somerae

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2974 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U7V468|U7V468_9FUSO tRNA nucleotidyltransferase/poly(A) polymerase family protein (Fragment) OS=Cetobacterium somerae ATCC BAA-474 OX=1319815 GN=HMPREF0202_02709 PE=3 SV=1
MM1 pKa = 7.37EE2 pKa = 5.17VIIMGNDD9 pKa = 3.13FFEE12 pKa = 4.91EE13 pKa = 4.09EE14 pKa = 4.3LNLSEE19 pKa = 5.57FLEE22 pKa = 4.7CTADD26 pKa = 3.63CSSFCIDD33 pKa = 4.88CTLGWNINLEE43 pKa = 4.23KK44 pKa = 10.22EE45 pKa = 4.47AKK47 pKa = 9.33NN48 pKa = 3.51

Molecular weight: 5.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U7VAW4|U7VAW4_9FUSO Uncharacterized protein OS=Cetobacterium somerae ATCC BAA-474 OX=1319815 GN=HMPREF0202_02166 PE=4 SV=1
MM1 pKa = 7.24NHH3 pKa = 5.69NKK5 pKa = 10.0SYY7 pKa = 10.99RR8 pKa = 11.84KK9 pKa = 9.26LGRR12 pKa = 11.84RR13 pKa = 11.84ADD15 pKa = 3.38HH16 pKa = 6.52RR17 pKa = 11.84KK18 pKa = 10.14AMLMNLTISLILSDD32 pKa = 5.03RR33 pKa = 11.84IEE35 pKa = 4.37TTVTRR40 pKa = 11.84AKK42 pKa = 10.12EE43 pKa = 3.68LRR45 pKa = 11.84KK46 pKa = 9.17FAEE49 pKa = 4.11RR50 pKa = 11.84MVTLGKK56 pKa = 10.47KK57 pKa = 8.62GTLAHH62 pKa = 6.67RR63 pKa = 11.84RR64 pKa = 11.84QAFAFLRR71 pKa = 11.84SEE73 pKa = 3.98EE74 pKa = 4.06AVAKK78 pKa = 10.17LFNDD82 pKa = 4.37LAPKK86 pKa = 9.15YY87 pKa = 10.33AEE89 pKa = 4.08RR90 pKa = 11.84NGGYY94 pKa = 7.87TRR96 pKa = 11.84IIRR99 pKa = 11.84TSVRR103 pKa = 11.84KK104 pKa = 9.93GDD106 pKa = 3.81SAEE109 pKa = 3.83MAIIEE114 pKa = 4.36LVV116 pKa = 3.14

Molecular weight: 13.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2974

0

2974

943243

29

4156

317.2

35.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.263 ± 0.047

0.871 ± 0.017

5.082 ± 0.035

7.702 ± 0.06

5.028 ± 0.042

6.811 ± 0.051

1.26 ± 0.015

9.791 ± 0.052

9.398 ± 0.058

9.754 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.456 ± 0.023

6.171 ± 0.049

2.875 ± 0.025

2.192 ± 0.02

3.178 ± 0.028

6.091 ± 0.032

4.978 ± 0.038

6.296 ± 0.034

0.701 ± 0.013

4.103 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski