Breoghania corrubedonensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Breoghaniaceae; Breoghania

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4681 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T5VGY0|A0A2T5VGY0_9RHIZ Transcription termination/antitermination protein NusA OS=Breoghania corrubedonensis OX=665038 GN=nusA PE=3 SV=1
MM1 pKa = 7.47IGAKK5 pKa = 9.4RR6 pKa = 11.84VLAAIGLLLAATIATAHH23 pKa = 6.15TADD26 pKa = 3.65AQPTFSAQFSPSTIAAGAEE45 pKa = 3.79SRR47 pKa = 11.84LIFTITNGSGSPVTAMTFTDD67 pKa = 3.97TLPAGTTVAASPRR80 pKa = 11.84GEE82 pKa = 4.26TTCIDD87 pKa = 3.36GVVTVNASDD96 pKa = 3.46TFTFEE101 pKa = 4.44GNGGASGASVAADD114 pKa = 3.52SSCTVSIYY122 pKa = 9.3VTASTGGAYY131 pKa = 10.77NNTSGDD137 pKa = 3.84LTSSAGNSGTASATLTVDD155 pKa = 3.81AARR158 pKa = 11.84LEE160 pKa = 4.07FDD162 pKa = 3.73MAFSPATVGIGAASRR177 pKa = 11.84ITYY180 pKa = 8.0TITKK184 pKa = 8.58PNSGVVTGVAFNNTLPTGLQVASPSSVATSCTGGAASATTGSQTVQLSGAFFTAAGSCTVTVDD247 pKa = 3.39VTNTGAGTYY256 pKa = 10.22VNSAEE261 pKa = 4.38LSSSAGTSGAATATLTVTAPTGDD284 pKa = 2.92ITLIKK289 pKa = 10.41QFARR293 pKa = 11.84TAAAPGGTIEE303 pKa = 4.14MTYY306 pKa = 10.03TLANRR311 pKa = 11.84SSSSAATAITFTDD324 pKa = 4.18DD325 pKa = 3.99LDD327 pKa = 4.16AALSGLTASALPATGFCGVGSQLTGSSTLTLSGATLGSRR366 pKa = 11.84EE367 pKa = 4.27SCSFTVTLDD376 pKa = 3.58VPASASDD383 pKa = 3.5TTVTSTTSSISGQLGGSPVTGDD405 pKa = 3.42PASASFNVVTTTPVMTKK422 pKa = 10.17SFTDD426 pKa = 3.71DD427 pKa = 3.42PVSAGGTVTLQYY439 pKa = 10.81VISNPSTITALGSIAFTDD457 pKa = 3.77SLAAMADD464 pKa = 3.48AAVTAGTGSNLCGSGSFLFTQEE486 pKa = 3.55ISGTPSVVLNSGTLAAGASCTLTLTLTLPANTPAGDD522 pKa = 3.88YY523 pKa = 10.88ASTSSDD529 pKa = 3.33VTSDD533 pKa = 3.12GGTGAPASDD542 pKa = 4.2TLQVSPPDD550 pKa = 3.6INVSLSKK557 pKa = 9.86TFTDD561 pKa = 3.94DD562 pKa = 3.21PVRR565 pKa = 11.84PGDD568 pKa = 3.67TVTLEE573 pKa = 3.71FTLRR577 pKa = 11.84NDD579 pKa = 4.4DD580 pKa = 3.74EE581 pKa = 4.48ATAITDD587 pKa = 4.38LAFTDD592 pKa = 5.23DD593 pKa = 4.54LDD595 pKa = 5.56AMLTGAVATGLPASNVCGTGSTVSGTSTIALSGGNLAASDD635 pKa = 3.88EE636 pKa = 4.78CTFSVTVQVPGGAAAGSYY654 pKa = 10.73PNTTSDD660 pKa = 3.16VSGDD664 pKa = 3.6SGSGIMTATGSAATGTLVVSDD685 pKa = 3.9VLAVTGTMSFTDD697 pKa = 3.96DD698 pKa = 3.73PVVAGSVVTLQYY710 pKa = 11.35VLSNPNTADD719 pKa = 3.66DD720 pKa = 3.77ATSISFSDD728 pKa = 3.6NLNGLGLPSPGLVLSGSLPSDD749 pKa = 3.25PCGTGSSLSLSSGGTAGTLSLSGGNILASGSCTFSIQFTVPSNAAANTYY798 pKa = 11.06GNTTSVISSTVNSTPLTSASGMNDD822 pKa = 3.17TLSVSSPYY830 pKa = 10.97EE831 pKa = 3.65NIIFTKK837 pKa = 10.42TFTDD841 pKa = 4.17DD842 pKa = 3.43PAEE845 pKa = 4.47AGSSVTLRR853 pKa = 11.84FVLDD857 pKa = 3.43NSVNSVAIDD866 pKa = 3.53GATFTDD872 pKa = 4.77DD873 pKa = 3.17LTAMGFAATVTADD886 pKa = 3.33SGSCSGGGSWNVTGTTTISASVSNLPGGATCTFDD920 pKa = 3.32VAVAIPSGTSPGISTNTTSTVAVTIGAGSGSVAAASDD957 pKa = 4.21DD958 pKa = 3.83LTISPTNVTLQKK970 pKa = 10.49SFSGDD975 pKa = 3.17PVLPGGTFSVTYY987 pKa = 9.42TILSPASGSALSDD1000 pKa = 2.84IRR1002 pKa = 11.84FTDD1005 pKa = 4.16DD1006 pKa = 2.84YY1007 pKa = 11.68DD1008 pKa = 3.94AVLSGLVVSGALPSNPCGAGSALAGTTSIEE1038 pKa = 4.12LTGGSLNPSEE1048 pKa = 4.42TCQFTVNLAAPSNATPNTYY1067 pKa = 9.98TSTSGALFEE1076 pKa = 4.45TAAQIAPPASDD1087 pKa = 3.19TFEE1090 pKa = 4.25VANPADD1096 pKa = 3.61NVTFALQFTDD1106 pKa = 5.26DD1107 pKa = 3.84PVAPGGTATAQFTITNPDD1125 pKa = 3.13SVSTVSGLAFTMPVDD1140 pKa = 3.89AVITGATVSSATPAAPCGGAVVSGTSSLSVSGGSLAAGANCSFTVSVLVPLGTTSGSNSFSTSTLAATANSTATTVSAASDD1221 pKa = 3.61TLVVGDD1227 pKa = 4.68APQFTVTSGDD1237 pKa = 3.67FNTSGAVGGPFNATMDD1253 pKa = 3.94YY1254 pKa = 10.68VISNTGSFPLSYY1266 pKa = 9.69TAAASQAFIGLSSTGGTIAAGGSQTVTVSITATANGFAASATPYY1310 pKa = 10.34SGTVTFTNISASGPPTEE1327 pKa = 4.3IRR1329 pKa = 11.84NVNLTVQQLGSVTVMVNSSQGDD1351 pKa = 3.74GTFTFSSSATALNGLAITTSNGAGSSAAVDD1381 pKa = 3.97VVAGSYY1387 pKa = 8.77TLSLPKK1393 pKa = 10.43AGMPDD1398 pKa = 3.44GFGLAGISCTEE1409 pKa = 3.94TGGTSNSSASVGSATATIVVEE1430 pKa = 3.98TGEE1433 pKa = 5.15AITCVFEE1440 pKa = 4.22TANSRR1445 pKa = 11.84EE1446 pKa = 3.95RR1447 pKa = 11.84TTAIINRR1454 pKa = 11.84FLSKK1458 pKa = 10.43RR1459 pKa = 11.84VDD1461 pKa = 4.41LILSNEE1467 pKa = 3.95PGQNRR1472 pKa = 11.84RR1473 pKa = 11.84IDD1475 pKa = 3.42RR1476 pKa = 11.84LRR1478 pKa = 11.84NRR1480 pKa = 11.84FGGGASGTGTPFDD1493 pKa = 3.69VTATQAGAGGVAMAFEE1509 pKa = 4.44TSLAQIRR1516 pKa = 11.84SAYY1519 pKa = 8.74VAQDD1523 pKa = 3.28RR1524 pKa = 11.84AKK1526 pKa = 10.23QALLATSGVFAHH1538 pKa = 6.43NPSGLPPEE1546 pKa = 4.09QTLWDD1551 pKa = 3.33VWFRR1555 pKa = 11.84GSFTWFEE1562 pKa = 4.64DD1563 pKa = 3.49NSDD1566 pKa = 3.83GSDD1569 pKa = 3.38SDD1571 pKa = 4.02GVFAIFHH1578 pKa = 6.77AGADD1582 pKa = 3.79YY1583 pKa = 10.87LVSDD1587 pKa = 5.44RR1588 pKa = 11.84ILVGALVQLDD1598 pKa = 3.95YY1599 pKa = 10.68MDD1601 pKa = 4.93QDD1603 pKa = 3.82FTTLNANANGVGWMAGPYY1621 pKa = 8.16ATLRR1625 pKa = 11.84LTNNLYY1631 pKa = 10.64FDD1633 pKa = 4.04TRR1635 pKa = 11.84AAWGTSFNQVSPFNTYY1651 pKa = 9.93TDD1653 pKa = 3.98DD1654 pKa = 4.13FSTTRR1659 pKa = 11.84WLVRR1663 pKa = 11.84AGLIGDD1669 pKa = 3.78WAFGAWNFRR1678 pKa = 11.84PSANVAYY1685 pKa = 9.03MQEE1688 pKa = 4.12TQEE1691 pKa = 4.78AYY1693 pKa = 9.34TDD1695 pKa = 3.86SLSVEE1700 pKa = 4.07IPEE1703 pKa = 4.01QTVGVGQLDD1712 pKa = 4.24FGPEE1716 pKa = 3.27ISYY1719 pKa = 8.75TYY1721 pKa = 8.61QTEE1724 pKa = 4.07TGYY1727 pKa = 11.22LIAPSAKK1734 pKa = 9.3LTGIWNFKK1742 pKa = 9.66RR1743 pKa = 11.84DD1744 pKa = 3.78TYY1746 pKa = 11.63GVTTTTSSAGVEE1758 pKa = 3.99MRR1760 pKa = 11.84AKK1762 pKa = 10.73AEE1764 pKa = 3.97LGLNVRR1770 pKa = 11.84MPSGVRR1776 pKa = 11.84VEE1778 pKa = 5.07ANGSYY1783 pKa = 10.75DD1784 pKa = 3.68GIGAEE1789 pKa = 5.07DD1790 pKa = 3.93YY1791 pKa = 10.89NAVSGQVKK1799 pKa = 10.31LSVPLNN1805 pKa = 3.64

Molecular weight: 179.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T5VF07|A0A2T5VF07_9RHIZ Dolichyl-phosphate-mannose-protein mannosyltransferase OS=Breoghania corrubedonensis OX=665038 GN=C8N35_101369 PE=4 SV=1
MM1 pKa = 6.85QRR3 pKa = 11.84LRR5 pKa = 11.84AGGNIAAGLAFVALAALFPATAFAACKK32 pKa = 9.64PPEE35 pKa = 4.45KK36 pKa = 9.66PAPPGCRR43 pKa = 11.84IKK45 pKa = 11.33GNINSDD51 pKa = 3.36GARR54 pKa = 11.84IYY56 pKa = 10.33HH57 pKa = 5.76VPGKK61 pKa = 9.86SRR63 pKa = 11.84WYY65 pKa = 10.71SRR67 pKa = 11.84TCINRR72 pKa = 11.84PGEE75 pKa = 3.77RR76 pKa = 11.84WFCSEE81 pKa = 4.01EE82 pKa = 3.79EE83 pKa = 3.83ARR85 pKa = 11.84AAGWRR90 pKa = 11.84APRR93 pKa = 11.84RR94 pKa = 3.71

Molecular weight: 10.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4681

0

4681

1532312

25

5123

327.3

35.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.466 ± 0.048

0.883 ± 0.013

5.937 ± 0.034

5.998 ± 0.039

3.716 ± 0.022

8.67 ± 0.042

2.005 ± 0.016

5.293 ± 0.028

3.289 ± 0.029

9.85 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.563 ± 0.019

2.577 ± 0.023

4.957 ± 0.025

2.858 ± 0.02

7.041 ± 0.041

5.468 ± 0.032

5.482 ± 0.044

7.498 ± 0.031

1.236 ± 0.015

2.213 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski