Salmonella phage S137

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 83 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z5HPH2|A0A2Z5HPH2_9CAUD Antitermination protein N OS=Salmonella phage S137 OX=2231359 PE=4 SV=1
MM1 pKa = 7.11ITGTSNYY8 pKa = 10.28DD9 pKa = 3.47EE10 pKa = 5.15VPTIPCKK17 pKa = 10.28ICGGYY22 pKa = 10.22FKK24 pKa = 11.04ADD26 pKa = 3.43DD27 pKa = 4.45PEE29 pKa = 4.21SHH31 pKa = 6.64KK32 pKa = 11.24CEE34 pKa = 4.61EE35 pKa = 4.38SDD37 pKa = 3.64QQ38 pKa = 3.74

Molecular weight: 4.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z5HPI2|A0A2Z5HPI2_9CAUD Primosomal protein I OS=Salmonella phage S137 OX=2231359 PE=4 SV=1
MM1 pKa = 7.02TRR3 pKa = 11.84RR4 pKa = 11.84TAFNGSAAGRR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84EE17 pKa = 4.32RR18 pKa = 11.84RR19 pKa = 11.84AALQNAVTASSEE31 pKa = 4.21VMHH34 pKa = 6.92RR35 pKa = 11.84PTLSRR40 pKa = 11.84AQIQAKK46 pKa = 9.67GKK48 pKa = 9.64HH49 pKa = 4.86EE50 pKa = 4.14TPKK53 pKa = 10.42RR54 pKa = 11.84IEE56 pKa = 4.21DD57 pKa = 3.59AKK59 pKa = 11.04SLQFMAKK66 pKa = 9.92DD67 pKa = 3.48AFWQLEE73 pKa = 4.22EE74 pKa = 3.9YY75 pKa = 10.34RR76 pKa = 11.84RR77 pKa = 11.84NLEE80 pKa = 3.59RR81 pKa = 11.84AAIVYY86 pKa = 10.58ANEE89 pKa = 4.43FGHH92 pKa = 6.59KK93 pKa = 9.71PPEE96 pKa = 4.36TGVCLPDD103 pKa = 3.32VALYY107 pKa = 10.45AAGHH111 pKa = 5.72RR112 pKa = 11.84KK113 pKa = 8.78CRR115 pKa = 11.84QVTARR120 pKa = 4.13

Molecular weight: 13.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

83

0

83

15196

37

1059

183.1

20.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.812 ± 0.412

1.296 ± 0.15

5.653 ± 0.193

7.008 ± 0.313

3.323 ± 0.173

7.12 ± 0.276

1.823 ± 0.161

5.758 ± 0.191

6.377 ± 0.346

7.502 ± 0.259

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.856 ± 0.136

4.916 ± 0.231

4.041 ± 0.248

3.988 ± 0.22

5.791 ± 0.286

6.752 ± 0.28

5.607 ± 0.314

6.41 ± 0.275

1.843 ± 0.113

3.126 ± 0.14
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski