Lake Sarah-associated circular virus-45

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140AQR0|A0A140AQR0_9VIRU Coat protein OS=Lake Sarah-associated circular virus-45 OX=1685774 PE=4 SV=1
MM1 pKa = 7.7PSFYY5 pKa = 10.48NGKK8 pKa = 9.18QFFLTYY14 pKa = 8.91PQCQHH19 pKa = 5.99SAAEE23 pKa = 4.0LAAFLTSVATCTYY36 pKa = 10.22YY37 pKa = 9.92IVAEE41 pKa = 4.35EE42 pKa = 4.04KK43 pKa = 10.89HH44 pKa = 6.34EE45 pKa = 4.86DD46 pKa = 3.8GTPHH50 pKa = 6.81LHH52 pKa = 7.11ACIQYY57 pKa = 9.68VEE59 pKa = 4.24TLRR62 pKa = 11.84GGVRR66 pKa = 11.84LLDD69 pKa = 4.1FNGHH73 pKa = 6.38HH74 pKa = 7.26PNKK77 pKa = 9.69QDD79 pKa = 3.16PRR81 pKa = 11.84KK82 pKa = 10.11FEE84 pKa = 4.01ACKK87 pKa = 10.16QYY89 pKa = 10.59CRR91 pKa = 11.84KK92 pKa = 10.21DD93 pKa = 3.28GNFIEE98 pKa = 5.38GPPEE102 pKa = 4.32AIIRR106 pKa = 11.84AAIAGLAPSDD116 pKa = 3.41IVKK119 pKa = 9.23TFTDD123 pKa = 3.27KK124 pKa = 11.36ALWFDD129 pKa = 3.13WCISKK134 pKa = 10.84RR135 pKa = 11.84ITHH138 pKa = 7.09GYY140 pKa = 9.2AEE142 pKa = 4.83WYY144 pKa = 7.2WNSTRR149 pKa = 11.84EE150 pKa = 3.78HH151 pKa = 7.36DD152 pKa = 3.79LTINEE157 pKa = 4.34DD158 pKa = 3.97TVVAGQMCEE167 pKa = 4.0SLAGYY172 pKa = 9.98AFNRR176 pKa = 11.84DD177 pKa = 3.18QHH179 pKa = 6.97RR180 pKa = 11.84VIVLKK185 pKa = 11.11GEE187 pKa = 4.29SGCGKK192 pKa = 5.88TTWAKK197 pKa = 8.25THH199 pKa = 6.23APKK202 pKa = 10.03PALFVSHH209 pKa = 7.21IDD211 pKa = 3.26SLKK214 pKa = 9.13RR215 pKa = 11.84FQPGFHH221 pKa = 6.29KK222 pKa = 9.66TIIFDD227 pKa = 4.03DD228 pKa = 3.8VDD230 pKa = 4.07FNHH233 pKa = 6.21YY234 pKa = 9.82PRR236 pKa = 11.84TGQIHH241 pKa = 5.53IVDD244 pKa = 4.19WEE246 pKa = 4.16NPRR249 pKa = 11.84AIHH252 pKa = 5.47VRR254 pKa = 11.84YY255 pKa = 8.18GTVEE259 pKa = 3.83IPAGTFKK266 pKa = 10.83IFTCNFDD273 pKa = 4.23PLTLTDD279 pKa = 3.54DD280 pKa = 5.07AIRR283 pKa = 11.84RR284 pKa = 11.84RR285 pKa = 11.84VKK287 pKa = 10.32VVNVNN292 pKa = 3.15

Molecular weight: 33.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A140AQR0|A0A140AQR0_9VIRU Coat protein OS=Lake Sarah-associated circular virus-45 OX=1685774 PE=4 SV=1
MM1 pKa = 7.16KK2 pKa = 10.22RR3 pKa = 11.84SRR5 pKa = 11.84KK6 pKa = 8.79VAYY9 pKa = 7.55NTPPNFDD16 pKa = 3.78SPMSTSAGRR25 pKa = 11.84IISLPRR31 pKa = 11.84RR32 pKa = 11.84GRR34 pKa = 11.84GRR36 pKa = 11.84SLSGRR41 pKa = 11.84GTLTPRR47 pKa = 11.84NLFGTVAKK55 pKa = 9.14TVASAHH61 pKa = 6.2PYY63 pKa = 9.53GRR65 pKa = 11.84AALNLYY71 pKa = 9.94RR72 pKa = 11.84GARR75 pKa = 11.84AISGIFKK82 pKa = 10.41SRR84 pKa = 11.84VVNTPGLKK92 pKa = 9.93RR93 pKa = 11.84RR94 pKa = 11.84TTGGPNARR102 pKa = 11.84FVGPFKK108 pKa = 10.84KK109 pKa = 9.75PKK111 pKa = 9.97KK112 pKa = 9.39LLKK115 pKa = 10.13PSMFATKK122 pKa = 10.5GFVHH126 pKa = 6.2TCEE129 pKa = 4.18INGTVSDD136 pKa = 4.82PDD138 pKa = 3.63CVYY141 pKa = 10.36IGHH144 pKa = 5.88STFSGIMTLEE154 pKa = 4.36LLCQVLLRR162 pKa = 11.84KK163 pKa = 9.98LFAKK167 pKa = 10.53AGIVLRR173 pKa = 11.84DD174 pKa = 3.23INEE177 pKa = 4.05PLRR180 pKa = 11.84GYY182 pKa = 9.28VANTSSMWRR191 pKa = 11.84IDD193 pKa = 4.18LIRR196 pKa = 11.84HH197 pKa = 4.93NKK199 pKa = 6.28EE200 pKa = 3.71TNVYY204 pKa = 10.04DD205 pKa = 3.6VAATFTTPSATNTNIYY221 pKa = 10.02QIVGDD226 pKa = 4.34RR227 pKa = 11.84RR228 pKa = 11.84ASSNGLFPGLYY239 pKa = 8.07NTLYY243 pKa = 10.71DD244 pKa = 3.82AAVGANGSDD253 pKa = 3.88NRR255 pKa = 11.84NVWQPSSLVLYY266 pKa = 10.14QEE268 pKa = 4.88EE269 pKa = 4.25EE270 pKa = 4.19NITQFFQFRR279 pKa = 11.84AEE281 pKa = 3.77IKK283 pKa = 9.91FDD285 pKa = 3.75NEE287 pKa = 4.1TVHH290 pKa = 6.67CFSKK294 pKa = 11.01SDD296 pKa = 3.5LKK298 pKa = 10.6VQNRR302 pKa = 11.84SVSASAGTTSDD313 pKa = 4.29AVDD316 pKa = 4.01ANPLMGKK323 pKa = 10.01LYY325 pKa = 9.98HH326 pKa = 6.66FKK328 pKa = 10.76GGCPRR333 pKa = 11.84SRR335 pKa = 11.84IVNAALVEE343 pKa = 4.39SVPDD347 pKa = 3.57LSGVITARR355 pKa = 11.84AASLVSAGAQAMSEE369 pKa = 4.21PPRR372 pKa = 11.84GNAWWNCSGTSGVKK386 pKa = 9.83LQPGHH391 pKa = 6.43IKK393 pKa = 10.35YY394 pKa = 10.74DD395 pKa = 3.28SVYY398 pKa = 9.19YY399 pKa = 9.79QNAMPILKK407 pKa = 9.85FLTAMNMASGPTGKK421 pKa = 10.07QIHH424 pKa = 6.46LFGKK428 pKa = 10.33SSLLALEE435 pKa = 5.13DD436 pKa = 4.0VINVNAAQNITCSYY450 pKa = 8.16EE451 pKa = 3.77CNRR454 pKa = 11.84RR455 pKa = 11.84YY456 pKa = 10.39GIYY459 pKa = 9.47LTTKK463 pKa = 9.23TVTFAQGLKK472 pKa = 10.54YY473 pKa = 10.6AITQNDD479 pKa = 3.63LTT481 pKa = 4.4

Molecular weight: 52.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

773

292

481

386.5

42.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.668 ± 0.234

2.199 ± 0.46

4.528 ± 0.852

3.622 ± 0.789

4.916 ± 0.65

7.374 ± 0.63

2.975 ± 1.126

5.692 ± 0.603

5.563 ± 0.135

7.115 ± 0.852

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.552 ± 0.452

5.692 ± 0.824

5.045 ± 0.048

3.105 ± 0.167

6.21 ± 0.38

6.598 ± 1.653

7.633 ± 0.051

6.468 ± 0.158

1.294 ± 0.396

3.752 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski