Sanxia sobemo-like virus 5

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEW7|A0A1L3KEW7_9VIRU C2H2-type domain-containing protein OS=Sanxia sobemo-like virus 5 OX=1923384 PE=4 SV=1
MM1 pKa = 7.92SYY3 pKa = 9.73EE4 pKa = 3.77HH5 pKa = 6.57YY6 pKa = 10.62EE7 pKa = 4.38RR8 pKa = 11.84VVQDD12 pKa = 4.56LDD14 pKa = 3.56WTSSPGYY21 pKa = 9.33PYY23 pKa = 11.21LLNAPTNGSFFQVQNGIPSEE43 pKa = 4.14EE44 pKa = 4.05AKK46 pKa = 10.71HH47 pKa = 6.2RR48 pKa = 11.84VWQLLTQRR56 pKa = 11.84LLDD59 pKa = 4.02RR60 pKa = 11.84SCDD63 pKa = 4.05PIRR66 pKa = 11.84LFIKK70 pKa = 10.31PEE72 pKa = 3.66PHH74 pKa = 6.13KK75 pKa = 10.48VAKK78 pKa = 10.36IEE80 pKa = 3.95TGRR83 pKa = 11.84FRR85 pKa = 11.84LISAVSVVDD94 pKa = 4.22QIIDD98 pKa = 3.43SMLFGEE104 pKa = 4.67MNKK107 pKa = 10.13NMVRR111 pKa = 11.84HH112 pKa = 5.91CLEE115 pKa = 4.2IPSKK119 pKa = 10.57FGWSPYY125 pKa = 8.31VGGWKK130 pKa = 9.76VVPQKK135 pKa = 11.1GLAIDD140 pKa = 3.6KK141 pKa = 10.77SSWDD145 pKa = 2.98WTMQGWLFQIILEE158 pKa = 4.18LRR160 pKa = 11.84SRR162 pKa = 11.84LCLTVGKK169 pKa = 9.73RR170 pKa = 11.84HH171 pKa = 6.49KK172 pKa = 10.06LWQEE176 pKa = 3.67LASWRR181 pKa = 11.84YY182 pKa = 8.13EE183 pKa = 3.77ALFGSPEE190 pKa = 4.31FILSNGLVLQQSQPGVMKK208 pKa = 10.24SGCVNTIADD217 pKa = 3.59NSIAQVVLHH226 pKa = 6.26LRR228 pKa = 11.84VCLEE232 pKa = 3.71MGIPAGWIWSMGDD245 pKa = 3.34DD246 pKa = 3.73TLQEE250 pKa = 4.1PLRR253 pKa = 11.84DD254 pKa = 3.59EE255 pKa = 4.74GSYY258 pKa = 10.39LASLSRR264 pKa = 11.84YY265 pKa = 9.6CIVKK269 pKa = 10.33EE270 pKa = 4.06STHH273 pKa = 7.01LIEE276 pKa = 4.94FAGHH280 pKa = 5.49QFRR283 pKa = 11.84KK284 pKa = 10.45DD285 pKa = 3.65SIEE288 pKa = 3.51PSYY291 pKa = 10.13FQKK294 pKa = 10.78HH295 pKa = 5.63CFNLLHH301 pKa = 7.06ADD303 pKa = 3.61SDD305 pKa = 4.51VVADD309 pKa = 4.41LAVSYY314 pKa = 11.27ALLYY318 pKa = 10.5HH319 pKa = 6.6KK320 pKa = 10.35SKK322 pKa = 10.73KK323 pKa = 9.56RR324 pKa = 11.84DD325 pKa = 4.25FIHH328 pKa = 6.68HH329 pKa = 6.67VIRR332 pKa = 11.84EE333 pKa = 4.19LGGIPASPRR342 pKa = 11.84MIAQIYY348 pKa = 8.79DD349 pKa = 3.89GPWW352 pKa = 2.67

Molecular weight: 40.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEW7|A0A1L3KEW7_9VIRU C2H2-type domain-containing protein OS=Sanxia sobemo-like virus 5 OX=1923384 PE=4 SV=1
MM1 pKa = 7.32VFGDD5 pKa = 3.93LTSCIRR11 pKa = 11.84MGYY14 pKa = 7.7NHH16 pKa = 6.51SFIRR20 pKa = 11.84RR21 pKa = 11.84FVVSAVATQLIIVSYY36 pKa = 10.49GFVSRR41 pKa = 11.84LAQWVVLVLSHH52 pKa = 7.32LLGPLFLGAVEE63 pKa = 4.7GIEE66 pKa = 3.98VGGIKK71 pKa = 9.88PYY73 pKa = 10.72KK74 pKa = 9.37LWLEE78 pKa = 4.38VGVEE82 pKa = 4.28PPRR85 pKa = 11.84PPAYY89 pKa = 9.83QRR91 pKa = 11.84LVDD94 pKa = 6.27GICEE98 pKa = 4.22WILNHH103 pKa = 5.53EE104 pKa = 4.56QEE106 pKa = 4.38TSMMLGVFACLVMLRR121 pKa = 11.84LLFKK125 pKa = 10.99LPVRR129 pKa = 11.84RR130 pKa = 11.84MSYY133 pKa = 9.87KK134 pKa = 10.06LRR136 pKa = 11.84GIRR139 pKa = 11.84FEE141 pKa = 4.23SVKK144 pKa = 10.64PGSEE148 pKa = 3.61LLAGVKK154 pKa = 10.07EE155 pKa = 4.27PAFQPRR161 pKa = 11.84IMQPGLFVNSFQGYY175 pKa = 8.85GIRR178 pKa = 11.84LGKK181 pKa = 9.7FLVMPRR187 pKa = 11.84HH188 pKa = 6.17VYY190 pKa = 9.45DD191 pKa = 3.44ACEE194 pKa = 4.19GQVMMRR200 pKa = 11.84GPKK203 pKa = 9.61SSYY206 pKa = 10.17IIANAPIQSRR216 pKa = 11.84VVRR219 pKa = 11.84DD220 pKa = 3.31LVYY223 pKa = 10.4IPLAEE228 pKa = 4.78KK229 pKa = 10.71VFSDD233 pKa = 4.55CGIPSATMARR243 pKa = 11.84RR244 pKa = 11.84LAKK247 pKa = 10.2GALVTCIGEE256 pKa = 4.13QGASSGIIRR265 pKa = 11.84PLSIMGMLSYY275 pKa = 10.22TGSTVPGMSGAAYY288 pKa = 9.17IVNSMSRR295 pKa = 11.84KK296 pKa = 7.77VHH298 pKa = 6.5GIHH301 pKa = 6.64HH302 pKa = 6.07GVVGEE307 pKa = 4.22NNVGTSSLIVAKK319 pKa = 9.22EE320 pKa = 3.67LKK322 pKa = 10.33ALEE325 pKa = 3.94GRR327 pKa = 11.84MFGEE331 pKa = 4.23SPLGNEE337 pKa = 4.63VEE339 pKa = 4.25EE340 pKa = 4.16QLADD344 pKa = 3.52RR345 pKa = 11.84RR346 pKa = 11.84GGYY349 pKa = 8.85KK350 pKa = 9.92AWAEE354 pKa = 4.0DD355 pKa = 3.88DD356 pKa = 3.87LDD358 pKa = 4.5EE359 pKa = 4.72VVLDD363 pKa = 3.62AWEE366 pKa = 4.95ADD368 pKa = 3.92DD369 pKa = 6.56DD370 pKa = 4.02DD371 pKa = 4.04WAYY374 pKa = 11.24DD375 pKa = 4.09DD376 pKa = 5.89DD377 pKa = 6.54AMDD380 pKa = 3.8YY381 pKa = 11.22EE382 pKa = 4.64EE383 pKa = 5.01DD384 pKa = 4.14VVWSRR389 pKa = 11.84EE390 pKa = 4.17SEE392 pKa = 5.32DD393 pKa = 3.32EE394 pKa = 4.36DD395 pKa = 4.04FWDD398 pKa = 4.02EE399 pKa = 5.12GPTRR403 pKa = 11.84WEE405 pKa = 3.73SRR407 pKa = 11.84KK408 pKa = 9.55RR409 pKa = 11.84RR410 pKa = 11.84PRR412 pKa = 11.84KK413 pKa = 8.55KK414 pKa = 8.56KK415 pKa = 8.92AVRR418 pKa = 11.84RR419 pKa = 11.84VVGLGHH425 pKa = 6.68AASRR429 pKa = 11.84PEE431 pKa = 3.67IEE433 pKa = 3.85ISGKK437 pKa = 9.94VKK439 pKa = 10.27HH440 pKa = 5.81QSGPQAQSKK449 pKa = 10.49ASPEE453 pKa = 3.77KK454 pKa = 10.25AAPPEE459 pKa = 4.06KK460 pKa = 9.79TVRR463 pKa = 11.84QAFNEE468 pKa = 3.95LEE470 pKa = 3.98ARR472 pKa = 11.84VSKK475 pKa = 10.95LEE477 pKa = 3.76KK478 pKa = 9.95RR479 pKa = 11.84VSSLEE484 pKa = 3.46ARR486 pKa = 11.84LPDD489 pKa = 3.61RR490 pKa = 11.84KK491 pKa = 9.96EE492 pKa = 3.76VAQKK496 pKa = 10.4VDD498 pKa = 3.31KK499 pKa = 10.83AVSAVRR505 pKa = 11.84TRR507 pKa = 11.84PEE509 pKa = 3.9PKK511 pKa = 9.63RR512 pKa = 11.84PPGSVVCMEE521 pKa = 4.69CKK523 pKa = 9.93RR524 pKa = 11.84RR525 pKa = 11.84FPHH528 pKa = 5.63VRR530 pKa = 11.84ALVAHH535 pKa = 6.78NDD537 pKa = 3.12EE538 pKa = 4.04KK539 pKa = 11.31HH540 pKa = 5.07RR541 pKa = 11.84VKK543 pKa = 11.04GEE545 pKa = 3.94SALKK549 pKa = 9.77EE550 pKa = 3.97DD551 pKa = 3.62SKK553 pKa = 11.64KK554 pKa = 10.55VVKK557 pKa = 7.02TTRR560 pKa = 11.84GRR562 pKa = 11.84FLGNSPKK569 pKa = 10.66NPGDD573 pKa = 3.68GPRR576 pKa = 11.84GSSKK580 pKa = 10.67LSPRR584 pKa = 11.84SSQFRR589 pKa = 11.84SILEE593 pKa = 4.35SQSKK597 pKa = 9.82MSDD600 pKa = 3.34CLCRR604 pKa = 11.84LEE606 pKa = 5.47KK607 pKa = 11.04SFDD610 pKa = 3.56LLARR614 pKa = 11.84AMAGPSSEE622 pKa = 3.88PRR624 pKa = 11.84RR625 pKa = 11.84NN626 pKa = 3.18

Molecular weight: 69.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

978

352

626

489.0

54.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.646 ± 0.933

1.738 ± 0.152

4.806 ± 0.187

6.748 ± 0.822

3.476 ± 0.305

7.566 ± 0.628

2.556 ± 0.692

5.112 ± 1.038

5.93 ± 0.497

9.1 ± 0.859

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.965 ± 0.249

2.249 ± 0.187

5.726 ± 0.373

3.681 ± 0.872

7.464 ± 1.258

8.793 ± 0.008

2.249 ± 0.187

8.384 ± 0.953

2.147 ± 0.768

2.658 ± 0.457
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski