Phycisphaerae bacterium RAS1

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Phycisphaerae; unclassified Phycisphaerae

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4315 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C5W7E7|A0A5C5W7E7_9BACT Co-chaperonin GroES OS=Phycisphaerae bacterium RAS1 OX=2528034 GN=RAS1_23850 PE=3 SV=1
MM1 pKa = 7.35LVCATPAAATDD12 pKa = 4.09VIDD15 pKa = 4.27CLGDD19 pKa = 3.8LVLGPAVPGFKK30 pKa = 10.14HH31 pKa = 6.47SVVADD36 pKa = 3.99PLTQATMIVGYY47 pKa = 8.03STGVTRR53 pKa = 11.84IDD55 pKa = 3.36GQYY58 pKa = 10.38GAFVICRR65 pKa = 11.84EE66 pKa = 4.13PEE68 pKa = 4.22GGVSLWVAIDD78 pKa = 3.61EE79 pKa = 4.69TTGAAALEE87 pKa = 4.3NDD89 pKa = 3.47WGADD93 pKa = 3.65LEE95 pKa = 4.65PTFQRR100 pKa = 11.84DD101 pKa = 3.27PSFGLAVADD110 pKa = 4.32WISANGAPAPGTTWDD125 pKa = 4.39LLYY128 pKa = 10.81HH129 pKa = 6.7ADD131 pKa = 3.6GVAGLGQLMGPAGAGGGDD149 pKa = 3.77PDD151 pKa = 5.49FGGVWGWLRR160 pKa = 11.84KK161 pKa = 9.68LICCLLSDD169 pKa = 4.94VPFCAPPPADD179 pKa = 5.39DD180 pKa = 5.7DD181 pKa = 5.17DD182 pKa = 7.04GDD184 pKa = 4.91GILNGDD190 pKa = 4.15DD191 pKa = 3.67NCPGVANPGQIDD203 pKa = 3.63TDD205 pKa = 3.53NDD207 pKa = 3.9GKK209 pKa = 11.63GNACDD214 pKa = 5.52DD215 pKa = 5.82DD216 pKa = 4.35IDD218 pKa = 4.93NDD220 pKa = 4.01GKK222 pKa = 10.1PNHH225 pKa = 6.29VDD227 pKa = 4.95DD228 pKa = 5.31DD229 pKa = 4.24TDD231 pKa = 5.55DD232 pKa = 5.79DD233 pKa = 5.13GDD235 pKa = 4.69PNGQDD240 pKa = 4.51DD241 pKa = 6.04DD242 pKa = 4.59IDD244 pKa = 5.78GDD246 pKa = 4.43GIPNHH251 pKa = 7.02LDD253 pKa = 2.95SDD255 pKa = 3.97MDD257 pKa = 4.16GDD259 pKa = 5.27GIPNYY264 pKa = 10.16LDD266 pKa = 3.58EE267 pKa = 7.15DD268 pKa = 3.83MDD270 pKa = 6.09GDD272 pKa = 4.2GLSNGLDD279 pKa = 3.54PDD281 pKa = 3.68IDD283 pKa = 4.22GDD285 pKa = 4.61GIPNTLDD292 pKa = 3.46PDD294 pKa = 3.63MDD296 pKa = 4.94GDD298 pKa = 4.17GATNADD304 pKa = 4.61DD305 pKa = 6.3DD306 pKa = 5.89DD307 pKa = 4.43MDD309 pKa = 6.36GDD311 pKa = 5.05GIDD314 pKa = 4.47DD315 pKa = 3.99AHH317 pKa = 7.81DD318 pKa = 3.83GDD320 pKa = 4.56IDD322 pKa = 5.61ADD324 pKa = 4.32GLPNWDD330 pKa = 5.27DD331 pKa = 3.97PDD333 pKa = 3.64MDD335 pKa = 4.32NDD337 pKa = 5.16GIANEE342 pKa = 4.96NDD344 pKa = 3.38DD345 pKa = 5.92DD346 pKa = 4.27MDD348 pKa = 6.35GDD350 pKa = 4.74GDD352 pKa = 4.94PNDD355 pKa = 5.51DD356 pKa = 5.0DD357 pKa = 6.3DD358 pKa = 6.77DD359 pKa = 5.91SEE361 pKa = 6.15GDD363 pKa = 3.45GDD365 pKa = 4.04GGGGGGALGCRR376 pKa = 11.84YY377 pKa = 9.16HH378 pKa = 7.02VCLEE382 pKa = 3.95GLRR385 pKa = 11.84LPVVSLSRR393 pKa = 11.84WPSGDD398 pKa = 3.34DD399 pKa = 3.41AQPGEE404 pKa = 5.26FGDD407 pKa = 3.65SWHH410 pKa = 6.8FGLGAEE416 pKa = 4.31PQFPCVSSIYY426 pKa = 10.21DD427 pKa = 4.02AIGLTIKK434 pKa = 10.61VAIQPPEE441 pKa = 4.16GACTRR446 pKa = 11.84DD447 pKa = 2.95AVLRR451 pKa = 11.84IRR453 pKa = 11.84ARR455 pKa = 11.84VGAAVVAEE463 pKa = 4.25GQLVFLLGAPPQDD476 pKa = 3.24RR477 pKa = 11.84ATLEE481 pKa = 4.12LATAAPLPAPAVGLLQRR498 pKa = 11.84WVVLDD503 pKa = 3.85EE504 pKa = 4.86SMDD507 pKa = 3.8GEE509 pKa = 4.11IWSPFAQATPSQWWIMVDD527 pKa = 3.42RR528 pKa = 11.84WPHH531 pKa = 4.33QLFGAIRR538 pKa = 11.84YY539 pKa = 9.4DD540 pKa = 3.61EE541 pKa = 5.36AVDD544 pKa = 4.28KK545 pKa = 11.15VAGYY549 pKa = 11.46ANGAQSYY556 pKa = 9.8SEE558 pKa = 3.65IAARR562 pKa = 11.84CTIGVDD568 pKa = 3.91SDD570 pKa = 3.2IKK572 pKa = 9.26YY573 pKa = 10.62NPMRR577 pKa = 11.84RR578 pKa = 11.84DD579 pKa = 3.32PSGHH583 pKa = 6.28ILSLYY588 pKa = 10.27RR589 pKa = 11.84LEE591 pKa = 4.62QGQCSAHH598 pKa = 6.21ARR600 pKa = 11.84LLTYY604 pKa = 10.73LLSCGGIDD612 pKa = 4.49AFTRR616 pKa = 11.84YY617 pKa = 8.65YY618 pKa = 10.32WSGSDD623 pKa = 3.11TLRR626 pKa = 11.84RR627 pKa = 11.84DD628 pKa = 3.01IYY630 pKa = 11.23NYY632 pKa = 10.31RR633 pKa = 11.84GWNATFQVEE642 pKa = 4.75RR643 pKa = 11.84PAHH646 pKa = 6.7DD647 pKa = 3.92GNEE650 pKa = 4.23LNPHH654 pKa = 6.47FSFHH658 pKa = 6.64CLTRR662 pKa = 11.84AEE664 pKa = 4.41LSGVLCDD671 pKa = 4.03PSYY674 pKa = 11.4GATGPLGILEE684 pKa = 4.67SGTAFSVDD692 pKa = 3.63AQGQPHH698 pKa = 7.06WPPAAGEE705 pKa = 4.03ALHH708 pKa = 6.38VPSILSGPAAPPEE721 pKa = 4.54RR722 pKa = 11.84IYY724 pKa = 11.5DD725 pKa = 3.59WTCPHH730 pKa = 7.02

Molecular weight: 77.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C5W3E5|A0A5C5W3E5_9BACT Succinate dehydrogenase flavoprotein subunit OS=Phycisphaerae bacterium RAS1 OX=2528034 GN=sdhA PE=4 SV=1
MM1 pKa = 7.18NKK3 pKa = 9.67KK4 pKa = 10.07GKK6 pKa = 9.83AAAAKK11 pKa = 9.37HH12 pKa = 4.94RR13 pKa = 11.84KK14 pKa = 8.31KK15 pKa = 10.22RR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84MKK20 pKa = 10.47ARR22 pKa = 11.84SKK24 pKa = 10.83ARR26 pKa = 11.84RR27 pKa = 11.84TAARR31 pKa = 11.84KK32 pKa = 9.16AA33 pKa = 3.34

Molecular weight: 3.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4315

0

4315

1565664

29

4597

362.8

39.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.271 ± 0.058

1.373 ± 0.021

5.98 ± 0.039

5.522 ± 0.028

3.503 ± 0.018

8.566 ± 0.04

1.979 ± 0.022

4.529 ± 0.024

2.785 ± 0.032

9.834 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.975 ± 0.016

2.838 ± 0.03

5.671 ± 0.032

3.27 ± 0.023

7.895 ± 0.045

5.52 ± 0.026

5.302 ± 0.029

7.366 ± 0.029

1.49 ± 0.018

2.332 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski