Gordonia phage Powerball

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Nymbaxtervirinae; unclassified Nymbaxtervirinae

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 76 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6TYK1|A0A5J6TYK1_9CAUD Minor tail protein OS=Gordonia phage Powerball OX=2599847 GN=26 PE=4 SV=1
MM1 pKa = 7.34FNCGMTIRR9 pKa = 11.84LVTAAAAAYY18 pKa = 9.01VALLIAGCSSDD29 pKa = 4.25DD30 pKa = 3.71EE31 pKa = 4.68GWRR34 pKa = 11.84TTAAEE39 pKa = 4.63GACEE43 pKa = 3.99DD44 pKa = 4.15SVRR47 pKa = 11.84AQMKK51 pKa = 10.57DD52 pKa = 2.98PDD54 pKa = 3.87SAEE57 pKa = 4.17FEE59 pKa = 4.48DD60 pKa = 4.37VTVVDD65 pKa = 4.95HH66 pKa = 7.62GDD68 pKa = 3.11GTYY71 pKa = 10.92SVTGQVNGRR80 pKa = 11.84NSFGGMAGFRR90 pKa = 11.84EE91 pKa = 4.81FTCAARR97 pKa = 11.84DD98 pKa = 4.1DD99 pKa = 4.48GDD101 pKa = 3.76QVTGRR106 pKa = 11.84AQLLGG111 pKa = 3.77

Molecular weight: 11.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6TRP6|A0A5J6TRP6_9CAUD Uncharacterized protein OS=Gordonia phage Powerball OX=2599847 GN=10 PE=4 SV=1
MM1 pKa = 6.65TTRR4 pKa = 11.84PCTRR8 pKa = 11.84TGCEE12 pKa = 3.4HH13 pKa = 7.07RR14 pKa = 11.84PRR16 pKa = 11.84SRR18 pKa = 11.84GLCHH22 pKa = 5.62THH24 pKa = 6.78YY25 pKa = 9.14MDD27 pKa = 6.13HH28 pKa = 6.62ITRR31 pKa = 11.84QKK33 pKa = 11.18AYY35 pKa = 10.13GRR37 pKa = 11.84WEE39 pKa = 4.01SSLVDD44 pKa = 3.54AEE46 pKa = 4.16PTRR49 pKa = 11.84QHH51 pKa = 5.98VEE53 pKa = 3.66RR54 pKa = 11.84LRR56 pKa = 11.84AAGMGTRR63 pKa = 11.84KK64 pKa = 9.54ISEE67 pKa = 4.23TAGVTRR73 pKa = 11.84SVLQSLLHH81 pKa = 5.77GKK83 pKa = 6.94TRR85 pKa = 11.84NGHH88 pKa = 6.0RR89 pKa = 11.84EE90 pKa = 4.03PPSKK94 pKa = 9.88TIAKK98 pKa = 9.77RR99 pKa = 11.84IADD102 pKa = 4.67SILAIPVPAQPLHH115 pKa = 6.35NGDD118 pKa = 3.52VPALGTRR125 pKa = 11.84RR126 pKa = 11.84RR127 pKa = 11.84LQALIAHH134 pKa = 7.62GYY136 pKa = 7.86SQAEE140 pKa = 3.93INRR143 pKa = 11.84RR144 pKa = 11.84FGLAADD150 pKa = 4.11NLTALIRR157 pKa = 11.84GEE159 pKa = 4.09RR160 pKa = 11.84NYY162 pKa = 10.52VDD164 pKa = 3.76PKK166 pKa = 7.62TARR169 pKa = 11.84RR170 pKa = 11.84VAALFRR176 pKa = 11.84EE177 pKa = 4.57LQLTPGPCTRR187 pKa = 11.84ARR189 pKa = 11.84NRR191 pKa = 11.84GKK193 pKa = 10.87ANGWPLPLDD202 pKa = 3.41WDD204 pKa = 4.19EE205 pKa = 5.38DD206 pKa = 4.47HH207 pKa = 7.44IDD209 pKa = 4.0DD210 pKa = 5.58PDD212 pKa = 3.71YY213 pKa = 10.43QAEE216 pKa = 4.05RR217 pKa = 11.84SEE219 pKa = 4.1VRR221 pKa = 11.84SIVRR225 pKa = 11.84DD226 pKa = 3.44PEE228 pKa = 4.53RR229 pKa = 11.84IEE231 pKa = 3.58WRR233 pKa = 11.84KK234 pKa = 9.71QKK236 pKa = 10.43VASLTAAGYY245 pKa = 9.27SAEE248 pKa = 4.42EE249 pKa = 3.47IAAQLRR255 pKa = 11.84CTARR259 pKa = 11.84TVQRR263 pKa = 11.84DD264 pKa = 3.38RR265 pKa = 11.84SDD267 pKa = 3.2LGLVEE272 pKa = 4.8RR273 pKa = 11.84GQVASS278 pKa = 3.82

Molecular weight: 31.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

76

0

76

15509

38

2137

204.1

22.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.58 ± 0.617

0.78 ± 0.153

6.97 ± 0.364

5.552 ± 0.25

2.392 ± 0.173

8.402 ± 0.407

2.121 ± 0.227

4.385 ± 0.152

3.179 ± 0.232

8.04 ± 0.243

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.128 ± 0.127

2.734 ± 0.234

5.461 ± 0.285

3.649 ± 0.365

7.531 ± 0.523

5.494 ± 0.298

7.396 ± 0.292

7.189 ± 0.25

1.87 ± 0.158

2.147 ± 0.165
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski