Equine herpesvirus 2 (strain 86/87) (EHV-2)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes; Herpesvirales; Herpesviridae; Gammaherpesvirinae; Percavirus; Equid gammaherpesvirus 2

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 78 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q66643|HEPA_EHV2 DNA helicase/primase complex-associated protein OS=Equine herpesvirus 2 (strain 86/87) OX=82831 GN=40 PE=3 SV=2
MM1 pKa = 7.79GILLSICRR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84HH12 pKa = 6.22DD13 pKa = 4.13PLTDD17 pKa = 3.65VEE19 pKa = 4.35GQPINVRR26 pKa = 11.84EE27 pKa = 4.0EE28 pKa = 4.08FEE30 pKa = 4.08MFEE33 pKa = 4.43EE34 pKa = 5.17GDD36 pKa = 3.96EE37 pKa = 4.11ATEE40 pKa = 4.01ACVFLNPNMGTDD52 pKa = 3.51EE53 pKa = 4.28YY54 pKa = 11.51DD55 pKa = 3.67EE56 pKa = 4.64EE57 pKa = 6.16DD58 pKa = 3.35EE59 pKa = 5.26GGDD62 pKa = 3.38GGEE65 pKa = 4.21GRR67 pKa = 11.84EE68 pKa = 4.11PQPEE72 pKa = 4.65VKK74 pKa = 7.8TTPYY78 pKa = 10.31PKK80 pKa = 9.81RR81 pKa = 11.84KK82 pKa = 9.58KK83 pKa = 9.94IKK85 pKa = 10.48LKK87 pKa = 10.86ADD89 pKa = 3.43VLL91 pKa = 4.13

Molecular weight: 10.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q66620|Q66620_EHV2 Capsid scaffold protein OS=Equine herpesvirus 2 (strain 86/87) OX=82831 GN=ORF17.5 PE=4 SV=1
MM1 pKa = 7.29FGVAKK6 pKa = 9.63PPPSPIPKK14 pKa = 8.91PPRR17 pKa = 11.84GRR19 pKa = 11.84HH20 pKa = 5.04FGEE23 pKa = 4.93KK24 pKa = 10.28YY25 pKa = 9.45PEE27 pKa = 4.25SVNFPSRR34 pKa = 11.84GARR37 pKa = 11.84QRR39 pKa = 11.84ASTRR43 pKa = 11.84HH44 pKa = 5.26RR45 pKa = 11.84PSIKK49 pKa = 10.18EE50 pKa = 3.69VWEE53 pKa = 4.43PPAPPGAGMIFWGEE67 pKa = 3.74NGEE70 pKa = 4.23NGDD73 pKa = 4.49FGQSAPSCYY82 pKa = 8.81EE83 pKa = 3.3RR84 pKa = 11.84RR85 pKa = 11.84RR86 pKa = 11.84ARR88 pKa = 11.84SQGGQGAEE96 pKa = 3.82NAAPVLRR103 pKa = 11.84PGPEE107 pKa = 3.31GRR109 pKa = 11.84RR110 pKa = 11.84RR111 pKa = 11.84RR112 pKa = 11.84EE113 pKa = 3.51SLIFLEE119 pKa = 4.43GDD121 pKa = 3.62MVRR124 pKa = 11.84EE125 pKa = 4.31TATVAPWTVSPLRR138 pKa = 11.84PEE140 pKa = 4.12RR141 pKa = 11.84PKK143 pKa = 11.11SLLGRR148 pKa = 11.84FRR150 pKa = 11.84SRR152 pKa = 11.84LTVWRR157 pKa = 11.84EE158 pKa = 3.7VPRR161 pKa = 11.84TPGPVAPIPEE171 pKa = 4.6LPGEE175 pKa = 4.37ADD177 pKa = 4.15DD178 pKa = 5.14PPTRR182 pKa = 11.84TPPPPPDD189 pKa = 3.65DD190 pKa = 3.62

Molecular weight: 20.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

77

1

78

38211

89

3755

489.9

53.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.165 ± 0.416

2.345 ± 0.201

4.847 ± 0.195

6.208 ± 0.374

4.475 ± 0.238

7.82 ± 0.34

2.327 ± 0.108

3.774 ± 0.183

4.329 ± 0.218

9.709 ± 0.317

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.07 ± 0.139

3.384 ± 0.163

7.071 ± 0.831

3.685 ± 0.334

6.045 ± 0.258

7.377 ± 0.213

5.284 ± 0.223

6.891 ± 0.263

1.015 ± 0.068

3.18 ± 0.183
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski