Amino acid dipepetide frequency for Acanthamoeba castellanii str. Neff

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	13.188AlaAla: 13.188 ± 0.084	1.251AlaCys: 1.251 ± 0.015	5.094AlaAsp: 5.094 ± 0.029	6.218AlaGlu: 6.218 ± 0.053	3.124AlaPhe: 3.124 ± 0.024	6.451AlaGly: 6.451 ± 0.042	2.345AlaHis: 2.345 ± 0.029	3.596AlaIle: 3.596 ± 0.029	4.965AlaLys: 4.965 ± 0.037	9.165AlaLeu: 9.165 ± 0.051	1.973AlaMet: 1.973 ± 0.019	2.727AlaAsn: 2.727 ± 0.023	5.258AlaPro: 5.258 ± 0.036	3.926AlaGln: 3.926 ± 0.031	5.577AlaArg: 5.577 ± 0.032	7.753AlaSer: 7.753 ± 0.042	6.127AlaThr: 6.127 ± 0.04	6.154AlaVal: 6.154 ± 0.043	1.099AlaTrp: 1.099 ± 0.016	2.115AlaTyr: 2.115 ± 0.021	0.0AlaXaa: 0.0 ± 0.0
Cys	1.181CysAla: 1.181 ± 0.014	0.366CysCys: 0.366 ± 0.009	0.692CysAsp: 0.692 ± 0.012	0.691CysGlu: 0.691 ± 0.011	0.597CysPhe: 0.597 ± 0.009	1.146CysGly: 1.146 ± 0.016	0.38CysHis: 0.38 ± 0.009	0.591CysIle: 0.591 ± 0.009	0.555CysLys: 0.555 ± 0.01	1.44CysLeu: 1.44 ± 0.017	0.321CysMet: 0.321 ± 0.007	0.455CysAsn: 0.455 ± 0.008	0.873CysPro: 0.873 ± 0.017	0.472CysGln: 0.472 ± 0.008	0.832CysArg: 0.832 ± 0.014	1.03CysSer: 1.03 ± 0.015	0.791CysThr: 0.791 ± 0.014	0.998CysVal: 0.998 ± 0.013	0.264CysTrp: 0.264 ± 0.007	0.404CysTyr: 0.404 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	4.822AspAla: 4.822 ± 0.033	0.733AspCys: 0.733 ± 0.013	5.527AspAsp: 5.527 ± 0.059	5.297AspGlu: 5.297 ± 0.041	1.98AspPhe: 1.98 ± 0.018	4.123AspGly: 4.123 ± 0.033	1.481AspHis: 1.481 ± 0.015	2.254AspIle: 2.254 ± 0.021	2.669AspLys: 2.669 ± 0.024	4.89AspLeu: 4.89 ± 0.031	1.149AspMet: 1.149 ± 0.015	1.79AspAsn: 1.79 ± 0.019	2.582AspPro: 2.582 ± 0.023	1.902AspGln: 1.902 ± 0.018	2.954AspArg: 2.954 ± 0.028	3.454AspSer: 3.454 ± 0.03	2.452AspThr: 2.452 ± 0.021	3.688AspVal: 3.688 ± 0.024	0.757AspTrp: 0.757 ± 0.012	1.532AspTyr: 1.532 ± 0.015	0.0AspXaa: 0.0 ± 0.0
Glu	6.928GluAla: 6.928 ± 0.053	0.828GluCys: 0.828 ± 0.012	4.38GluAsp: 4.38 ± 0.036	9.145GluGlu: 9.145 ± 0.121	1.858GluPhe: 1.858 ± 0.016	4.789GluGly: 4.789 ± 0.034	1.472GluHis: 1.472 ± 0.017	2.359GluIle: 2.359 ± 0.022	4.599GluLys: 4.599 ± 0.051	6.168GluLeu: 6.168 ± 0.042	1.46GluMet: 1.46 ± 0.017	1.8GluAsn: 1.8 ± 0.017	2.465GluPro: 2.465 ± 0.022	2.992GluGln: 2.992 ± 0.032	5.191GluArg: 5.191 ± 0.054	3.658GluSer: 3.658 ± 0.029	2.805GluThr: 2.805 ± 0.025	4.4GluVal: 4.4 ± 0.032	1.017GluTrp: 1.017 ± 0.013	1.37GluTyr: 1.37 ± 0.016	0.0GluXaa: 0.0 ± 0.0
Phe	3.103PheAla: 3.103 ± 0.027	0.618PheCys: 0.618 ± 0.01	2.069PheAsp: 2.069 ± 0.017	1.928PheGlu: 1.928 ± 0.019	1.611PhePhe: 1.611 ± 0.019	2.579PheGly: 2.579 ± 0.027	0.905PheHis: 0.905 ± 0.013	1.594PheIle: 1.594 ± 0.018	1.51PheLys: 1.51 ± 0.018	3.545PheLeu: 3.545 ± 0.028	0.775PheMet: 0.775 ± 0.01	1.323PheAsn: 1.323 ± 0.015	1.676PhePro: 1.676 ± 0.017	1.091PheGln: 1.091 ± 0.014	1.684PheArg: 1.684 ± 0.017	2.549PheSer: 2.549 ± 0.025	1.953PheThr: 1.953 ± 0.019	2.678PheVal: 2.678 ± 0.023	0.475PheTrp: 0.475 ± 0.008	1.102PheTyr: 1.102 ± 0.014	0.0PheXaa: 0.0 ± 0.0
Gly	6.065GlyAla: 6.065 ± 0.039	0.974GlyCys: 0.974 ± 0.015	4.129GlyAsp: 4.129 ± 0.027	4.538GlyGlu: 4.538 ± 0.032	2.24GlyPhe: 2.24 ± 0.018	7.798GlyGly: 7.798 ± 0.083	1.831GlyHis: 1.831 ± 0.019	2.404GlyIle: 2.404 ± 0.023	3.581GlyLys: 3.581 ± 0.029	5.859GlyLeu: 5.859 ± 0.038	1.426GlyMet: 1.426 ± 0.019	2.077GlyAsn: 2.077 ± 0.021	2.738GlyPro: 2.738 ± 0.025	2.608GlyGln: 2.608 ± 0.025	4.407GlyArg: 4.407 ± 0.032	5.49GlySer: 5.49 ± 0.042	3.429GlyThr: 3.429 ± 0.027	4.529GlyVal: 4.529 ± 0.034	1.003GlyTrp: 1.003 ± 0.013	1.75GlyTyr: 1.75 ± 0.021	0.0GlyXaa: 0.0 ± 0.0
His	2.165HisAla: 2.165 ± 0.032	0.419HisCys: 0.419 ± 0.008	1.319HisAsp: 1.319 ± 0.015	1.377HisGlu: 1.377 ± 0.014	1.051HisPhe: 1.051 ± 0.014	1.713HisGly: 1.713 ± 0.017	1.589HisHis: 1.589 ± 0.03	1.071HisIle: 1.071 ± 0.012	1.161HisLys: 1.161 ± 0.014	2.675HisLeu: 2.675 ± 0.019	0.558HisMet: 0.558 ± 0.009	0.935HisAsn: 0.935 ± 0.012	1.584HisPro: 1.584 ± 0.02	1.383HisGln: 1.383 ± 0.021	1.71HisArg: 1.71 ± 0.019	1.797HisSer: 1.797 ± 0.02	1.365HisThr: 1.365 ± 0.017	1.56HisVal: 1.56 ± 0.015	0.347HisTrp: 0.347 ± 0.007	0.853HisTyr: 0.853 ± 0.012	0.0HisXaa: 0.0 ± 0.0
Ile	3.425IleAla: 3.425 ± 0.023	0.553IleCys: 0.553 ± 0.01	2.393IleAsp: 2.393 ± 0.019	2.488IleGlu: 2.488 ± 0.02	1.486IlePhe: 1.486 ± 0.017	2.28IleGly: 2.28 ± 0.02	0.977IleHis: 0.977 ± 0.013	1.82IleIle: 1.82 ± 0.02	2.151IleLys: 2.151 ± 0.021	3.257IleLeu: 3.257 ± 0.025	0.806IleMet: 0.806 ± 0.011	1.631IleAsn: 1.631 ± 0.015	1.921IlePro: 1.921 ± 0.017	1.316IleGln: 1.316 ± 0.014	2.054IleArg: 2.054 ± 0.019	2.549IleSer: 2.549 ± 0.023	2.434IleThr: 2.434 ± 0.025	2.779IleVal: 2.779 ± 0.024	0.415IleTrp: 0.415 ± 0.008	1.098IleTyr: 1.098 ± 0.014	0.0IleXaa: 0.0 ± 0.0
Lys	4.825LysAla: 4.825 ± 0.039	0.605LysCys: 0.605 ± 0.01	2.696LysAsp: 2.696 ± 0.025	4.895LysGlu: 4.895 ± 0.051	1.293LysPhe: 1.293 ± 0.015	3.218LysGly: 3.218 ± 0.028	1.192LysHis: 1.192 ± 0.015	1.859LysIle: 1.859 ± 0.02	5.222LysLys: 5.222 ± 0.064	4.379LysLeu: 4.379 ± 0.03	1.104LysMet: 1.104 ± 0.014	1.696LysAsn: 1.696 ± 0.019	2.343LysPro: 2.343 ± 0.023	2.347LysGln: 2.347 ± 0.025	4.304LysArg: 4.304 ± 0.037	2.969LysSer: 2.969 ± 0.026	2.559LysThr: 2.559 ± 0.026	2.952LysVal: 2.952 ± 0.024	0.692LysTrp: 0.692 ± 0.01	1.232LysTyr: 1.232 ± 0.015	0.001LysXaa: 0.001 ± 0.0
Leu	9.298LeuAla: 9.298 ± 0.054	1.517LeuCys: 1.517 ± 0.016	4.855LeuAsp: 4.855 ± 0.032	5.538LeuGlu: 5.538 ± 0.037	3.582LeuPhe: 3.582 ± 0.028	5.689LeuGly: 5.689 ± 0.038	2.807LeuHis: 2.807 ± 0.024	3.37LeuIle: 3.37 ± 0.027	4.406LeuLys: 4.406 ± 0.034	10.096LeuLeu: 10.096 ± 0.059	1.952LeuMet: 1.952 ± 0.017	2.926LeuAsn: 2.926 ± 0.021	5.466LeuPro: 5.466 ± 0.034	3.894LeuGln: 3.894 ± 0.031	6.305LeuArg: 6.305 ± 0.033	6.749LeuSer: 6.749 ± 0.04	4.902LeuThr: 4.902 ± 0.031	6.476LeuVal: 6.476 ± 0.041	1.287LeuTrp: 1.287 ± 0.018	2.413LeuTyr: 2.413 ± 0.024	0.001LeuXaa: 0.001 ± 0.0
Met	2.135MetAla: 2.135 ± 0.015	0.304MetCys: 0.304 ± 0.007	1.133MetAsp: 1.133 ± 0.013	1.643MetGlu: 1.643 ± 0.016	0.632MetPhe: 0.632 ± 0.01	1.449MetGly: 1.449 ± 0.017	0.504MetHis: 0.504 ± 0.008	0.72MetIle: 0.72 ± 0.01	1.171MetLys: 1.171 ± 0.014	1.892MetLeu: 1.892 ± 0.018	0.61MetMet: 0.61 ± 0.011	0.662MetAsn: 0.662 ± 0.011	1.068MetPro: 1.068 ± 0.016	0.896MetGln: 0.896 ± 0.013	1.368MetArg: 1.368 ± 0.014	1.438MetSer: 1.438 ± 0.016	1.166MetThr: 1.166 ± 0.013	1.33MetVal: 1.33 ± 0.016	0.325MetTrp: 0.325 ± 0.007	0.429MetTyr: 0.429 ± 0.008	0.0MetXaa: 0.0 ± 0.0
Asn	2.78AsnAla: 2.78 ± 0.021	0.449AsnCys: 0.449 ± 0.009	1.804AsnAsp: 1.804 ± 0.018	2.05AsnGlu: 2.05 ± 0.018	1.209AsnPhe: 1.209 ± 0.016	2.305AsnGly: 2.305 ± 0.023	0.775AsnHis: 0.775 ± 0.012	1.46AsnIle: 1.46 ± 0.015	1.791AsnLys: 1.791 ± 0.02	2.834AsnLeu: 2.834 ± 0.024	0.685AsnMet: 0.685 ± 0.012	1.464AsnAsn: 1.464 ± 0.023	1.69AsnPro: 1.69 ± 0.018	1.116AsnGln: 1.116 ± 0.013	1.638AsnArg: 1.638 ± 0.014	2.069AsnSer: 2.069 ± 0.024	1.735AsnThr: 1.735 ± 0.025	2.172AsnVal: 2.172 ± 0.023	0.442AsnTrp: 0.442 ± 0.008	0.961AsnTyr: 0.961 ± 0.014	0.0AsnXaa: 0.0 ± 0.0
Pro	6.046ProAla: 6.046 ± 0.037	0.579ProCys: 0.579 ± 0.011	2.523ProAsp: 2.523 ± 0.021	3.017ProGlu: 3.017 ± 0.022	1.91ProPhe: 1.91 ± 0.02	3.022ProGly: 3.022 ± 0.025	1.561ProHis: 1.561 ± 0.017	1.87ProIle: 1.87 ± 0.018	2.169ProLys: 2.169 ± 0.022	4.846ProLeu: 4.846 ± 0.029	0.969ProMet: 0.969 ± 0.013	1.6ProAsn: 1.6 ± 0.015	5.149ProPro: 5.149 ± 0.056	2.267ProGln: 2.267 ± 0.023	3.159ProArg: 3.159 ± 0.027	5.654ProSer: 5.654 ± 0.063	3.816ProThr: 3.816 ± 0.046	3.23ProVal: 3.23 ± 0.025	0.586ProTrp: 0.586 ± 0.011	1.309ProTyr: 1.309 ± 0.018	0.001ProXaa: 0.001 ± 0.0
Gln	3.831GlnAla: 3.831 ± 0.032	0.511GlnCys: 0.511 ± 0.009	1.56GlnAsp: 1.56 ± 0.018	2.613GlnGlu: 2.613 ± 0.029	1.287GlnPhe: 1.287 ± 0.014	2.251GlnGly: 2.251 ± 0.022	1.423GlnHis: 1.423 ± 0.02	1.43GlnIle: 1.43 ± 0.017	2.014GlnLys: 2.014 ± 0.022	4.28GlnLeu: 4.28 ± 0.031	0.905GlnMet: 0.905 ± 0.014	0.988GlnAsn: 0.988 ± 0.013	2.614GlnPro: 2.614 ± 0.027	3.73GlnGln: 3.73 ± 0.057	3.079GlnArg: 3.079 ± 0.025	2.348GlnSer: 2.348 ± 0.026	1.824GlnThr: 1.824 ± 0.017	2.335GlnVal: 2.335 ± 0.021	0.586GlnTrp: 0.586 ± 0.01	0.937GlnTyr: 0.937 ± 0.012	0.0GlnXaa: 0.0 ± 0.0
Arg	5.385ArgAla: 5.385 ± 0.037	0.844ArgCys: 0.844 ± 0.013	3.391ArgAsp: 3.391 ± 0.029	4.87ArgGlu: 4.87 ± 0.051	2.099ArgPhe: 2.099 ± 0.02	4.086ArgGly: 4.086 ± 0.036	1.734ArgHis: 1.734 ± 0.019	2.226ArgIle: 2.226 ± 0.019	3.855ArgLys: 3.855 ± 0.035	6.087ArgLeu: 6.087 ± 0.036	1.289ArgMet: 1.289 ± 0.015	1.821ArgAsn: 1.821 ± 0.015	3.281ArgPro: 3.281 ± 0.027	2.766ArgGln: 2.766 ± 0.026	5.858ArgArg: 5.858 ± 0.046	4.42ArgSer: 4.42 ± 0.031	3.113ArgThr: 3.113 ± 0.022	3.82ArgVal: 3.82 ± 0.027	0.936ArgTrp: 0.936 ± 0.012	1.506ArgTyr: 1.506 ± 0.015	0.001ArgXaa: 0.001 ± 0.0
Ser	7.449SerAla: 7.449 ± 0.046	0.979SerCys: 0.979 ± 0.013	3.725SerAsp: 3.725 ± 0.032	3.703SerGlu: 3.703 ± 0.031	2.896SerPhe: 2.896 ± 0.024	5.503SerGly: 5.503 ± 0.036	1.602SerHis: 1.602 ± 0.016	2.638SerIle: 2.638 ± 0.021	2.949SerLys: 2.949 ± 0.025	7.002SerLeu: 7.002 ± 0.046	1.43SerMet: 1.43 ± 0.016	2.131SerAsn: 2.131 ± 0.028	5.616SerPro: 5.616 ± 0.065	2.258SerGln: 2.258 ± 0.021	3.934SerArg: 3.934 ± 0.033	10.859SerSer: 10.859 ± 0.105	4.933SerThr: 4.933 ± 0.037	4.326SerVal: 4.326 ± 0.028	0.931SerTrp: 0.931 ± 0.013	1.697SerTyr: 1.697 ± 0.018	0.001SerXaa: 0.001 ± 0.0
Thr	5.531ThrAla: 5.531 ± 0.036	0.785ThrCys: 0.785 ± 0.012	2.615ThrAsp: 2.615 ± 0.024	2.932ThrGlu: 2.932 ± 0.023	2.032ThrPhe: 2.032 ± 0.02	3.472ThrGly: 3.472 ± 0.028	1.328ThrHis: 1.328 ± 0.015	2.45ThrIle: 2.45 ± 0.021	2.753ThrLys: 2.753 ± 0.022	5.029ThrLeu: 5.029 ± 0.031	1.092ThrMet: 1.092 ± 0.013	1.898ThrAsn: 1.898 ± 0.019	3.771ThrPro: 3.771 ± 0.045	1.876ThrGln: 1.876 ± 0.023	3.007ThrArg: 3.007 ± 0.021	4.963ThrSer: 4.963 ± 0.036	5.222ThrThr: 5.222 ± 0.058	3.372ThrVal: 3.372 ± 0.028	0.706ThrTrp: 0.706 ± 0.011	1.386ThrTyr: 1.386 ± 0.015	0.0ThrXaa: 0.0 ± 0.0
Val	6.655ValAla: 6.655 ± 0.044	1.059ValCys: 1.059 ± 0.015	3.822ValAsp: 3.822 ± 0.023	4.466ValGlu: 4.466 ± 0.029	2.308ValPhe: 2.308 ± 0.022	4.388ValGly: 4.388 ± 0.031	1.584ValHis: 1.584 ± 0.017	2.581ValIle: 2.581 ± 0.022	3.055ValLys: 3.055 ± 0.027	6.287ValLeu: 6.287 ± 0.039	1.476ValMet: 1.476 ± 0.015	1.991ValAsn: 1.991 ± 0.02	3.398ValPro: 3.398 ± 0.025	2.259ValGln: 2.259 ± 0.02	3.738ValArg: 3.738 ± 0.025	4.185ValSer: 4.185 ± 0.028	3.394ValThr: 3.394 ± 0.033	5.814ValVal: 5.814 ± 0.042	0.977ValTrp: 0.977 ± 0.015	1.689ValTyr: 1.689 ± 0.02	0.0ValXaa: 0.0 ± 0.0
Trp	1.115TrpAla: 1.115 ± 0.016	0.228TrpCys: 0.228 ± 0.006	0.806TrpAsp: 0.806 ± 0.012	0.866TrpGlu: 0.866 ± 0.011	0.44TrpPhe: 0.44 ± 0.009	0.808TrpGly: 0.808 ± 0.011	0.387TrpHis: 0.387 ± 0.009	0.515TrpIle: 0.515 ± 0.011	0.742TrpLys: 0.742 ± 0.01	1.322TrpLeu: 1.322 ± 0.017	0.349TrpMet: 0.349 ± 0.008	0.548TrpAsn: 0.548 ± 0.01	0.593TrpPro: 0.593 ± 0.012	0.524TrpGln: 0.524 ± 0.01	1.07TrpArg: 1.07 ± 0.014	0.889TrpSer: 0.889 ± 0.013	0.8TrpThr: 0.8 ± 0.012	0.879TrpVal: 0.879 ± 0.012	0.308TrpTrp: 0.308 ± 0.008	0.336TrpTyr: 0.336 ± 0.008	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.175TyrAla: 2.175 ± 0.024	0.474TyrCys: 0.474 ± 0.008	1.501TyrAsp: 1.501 ± 0.014	1.409TyrGlu: 1.409 ± 0.016	1.132TyrPhe: 1.132 ± 0.014	1.804TyrGly: 1.804 ± 0.022	0.755TyrHis: 0.755 ± 0.011	1.026TyrIle: 1.026 ± 0.014	1.066TyrLys: 1.066 ± 0.013	2.477TyrLeu: 2.477 ± 0.022	0.55TyrMet: 0.55 ± 0.01	0.965TyrAsn: 0.965 ± 0.013	1.197TyrPro: 1.197 ± 0.016	0.923TyrGln: 0.923 ± 0.012	1.518TyrArg: 1.518 ± 0.018	1.739TyrSer: 1.739 ± 0.019	1.401TyrThr: 1.401 ± 0.016	1.635TyrVal: 1.635 ± 0.016	0.373TyrTrp: 0.373 ± 0.008	0.95TyrTyr: 0.95 ± 0.015	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.0	0.001XaaSer: 0.001 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.001XaaVal: 0.001 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.04XaaXaa: 0.04 ± 0.01

Statistics based on 14939 proteins (6560542 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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