Clostridium phage phiCT453B

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 61 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0A7RTJ3|A0A0A7RTJ3_9CAUD Uncharacterized protein OS=Clostridium phage phiCT453B OX=1567013 GN=phiCT453B_13 PE=4 SV=1
MM1 pKa = 8.11DD2 pKa = 5.74NLPDD6 pKa = 3.55CQYY9 pKa = 10.65EE10 pKa = 4.05YY11 pKa = 10.93GYY13 pKa = 9.96EE14 pKa = 4.07SPIMQIAEE22 pKa = 3.91EE23 pKa = 4.25CSICGHH29 pKa = 6.73EE30 pKa = 4.41IYY32 pKa = 10.71EE33 pKa = 4.37GDD35 pKa = 3.67GYY37 pKa = 10.59YY38 pKa = 10.56HH39 pKa = 7.38IDD41 pKa = 3.59NLNICEE47 pKa = 4.22DD48 pKa = 4.56CILDD52 pKa = 3.72FKK54 pKa = 11.29KK55 pKa = 10.4IGEE58 pKa = 4.07II59 pKa = 3.91

Molecular weight:
6.86 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A0A7RTL0|A0A0A7RTL0_9CAUD Uncharacterized protein OS=Clostridium phage phiCT453B OX=1567013 GN=phiCT453B_07 PE=4 SV=1
MM1 pKa = 7.61ALKK4 pKa = 10.24KK5 pKa = 10.43LCPKK9 pKa = 9.91CGCLISMTEE18 pKa = 4.15KK19 pKa = 10.71LCQKK23 pKa = 10.27CMPKK27 pKa = 10.18YY28 pKa = 8.43EE29 pKa = 4.09QEE31 pKa = 4.1KK32 pKa = 10.56KK33 pKa = 10.54EE34 pKa = 4.33SNKK37 pKa = 10.59LYY39 pKa = 10.51DD40 pKa = 3.02RR41 pKa = 11.84RR42 pKa = 11.84YY43 pKa = 8.1RR44 pKa = 11.84NKK46 pKa = 10.04RR47 pKa = 11.84STEE50 pKa = 4.27FYY52 pKa = 10.41HH53 pKa = 6.39SKK55 pKa = 9.5SWLIVRR61 pKa = 11.84EE62 pKa = 4.1QIRR65 pKa = 11.84QRR67 pKa = 11.84DD68 pKa = 3.48NGLCKK73 pKa = 10.54LCLRR77 pKa = 11.84NKK79 pKa = 10.06KK80 pKa = 8.95IRR82 pKa = 11.84SMDD85 pKa = 3.44MVHH88 pKa = 7.3HH89 pKa = 6.35IEE91 pKa = 4.17EE92 pKa = 4.52LRR94 pKa = 11.84EE95 pKa = 4.07KK96 pKa = 10.48YY97 pKa = 10.15NKK99 pKa = 10.1RR100 pKa = 11.84LGKK103 pKa = 10.36DD104 pKa = 3.33NLICLCNKK112 pKa = 9.04CHH114 pKa = 6.27NAVHH118 pKa = 6.81AEE120 pKa = 3.82YY121 pKa = 11.26SNGNKK126 pKa = 10.35ASMQEE131 pKa = 3.92EE132 pKa = 4.45LRR134 pKa = 11.84RR135 pKa = 11.84LIDD138 pKa = 3.27RR139 pKa = 11.84RR140 pKa = 11.84GYY142 pKa = 10.34

Molecular weight:
17.13 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

61

0

61

11078

39

859

181.6

20.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.019 ± 0.509

1.327 ± 0.18

5.831 ± 0.282

8.494 ± 0.446

3.647 ± 0.242

5.579 ± 0.33

1.083 ± 0.161

9.307 ± 0.248

11.284 ± 0.275

8.431 ± 0.306

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.582 ± 0.173

7.285 ± 0.372

2.23 ± 0.152

2.871 ± 0.147

3.782 ± 0.32

5.109 ± 0.232

5.497 ± 0.37

5.326 ± 0.346

1.101 ± 0.11

4.216 ± 0.309

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski