Mycobacterium phage Lolly9

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bronvirus; unclassified Bronvirus

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 130 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K2FNS1|A0A0K2FNS1_9CAUD Tail assembly chaperone OS=Mycobacterium phage Lolly9 OX=1698711 GN=15 PE=4 SV=1
MM1 pKa = 7.65FYY3 pKa = 9.77MDD5 pKa = 3.91SAEE8 pKa = 4.98QVAQDD13 pKa = 3.37IADD16 pKa = 3.52MHH18 pKa = 6.25SAYY21 pKa = 9.9MDD23 pKa = 4.37DD24 pKa = 4.26YY25 pKa = 11.58AEE27 pKa = 4.55HH28 pKa = 7.08RR29 pKa = 11.84ADD31 pKa = 3.26TDD33 pKa = 3.64YY34 pKa = 11.59SADD37 pKa = 4.23EE38 pKa = 4.24IADD41 pKa = 3.44MYY43 pKa = 11.09AWQEE47 pKa = 4.04SALMVAGGSAVPSDD61 pKa = 2.84IGARR65 pKa = 11.84AEE67 pKa = 4.52LIAHH71 pKa = 6.61IAGYY75 pKa = 10.29

Molecular weight: 8.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K2FMM9|A0A0K2FMM9_9CAUD Uncharacterized protein OS=Mycobacterium phage Lolly9 OX=1698711 GN=50 PE=4 SV=1
MM1 pKa = 7.15TRR3 pKa = 11.84NEE5 pKa = 3.98LAKK8 pKa = 10.43VLGVARR14 pKa = 11.84STVYY18 pKa = 10.62GAFAEE23 pKa = 4.49DD24 pKa = 3.47WTGEE28 pKa = 3.96PSIKK32 pKa = 9.85MLAQMAGYY40 pKa = 9.26FRR42 pKa = 11.84VPIGSLVTEE51 pKa = 5.1PGRR54 pKa = 11.84KK55 pKa = 7.78AAKK58 pKa = 9.59RR59 pKa = 11.84EE60 pKa = 3.9SRR62 pKa = 11.84RR63 pKa = 11.84VNVRR67 pKa = 11.84RR68 pKa = 11.84IAA70 pKa = 3.41

Molecular weight: 7.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

130

0

130

22756

34

1747

175.0

19.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.7 ± 0.29

1.125 ± 0.159

6.53 ± 0.187

5.748 ± 0.275

3.309 ± 0.098

8.398 ± 0.36

1.925 ± 0.149

4.144 ± 0.128

4.904 ± 0.218

8.552 ± 0.208

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.461 ± 0.115

3.753 ± 0.204

5.106 ± 0.244

3.669 ± 0.145

6.02 ± 0.289

5.546 ± 0.189

5.744 ± 0.219

7.242 ± 0.217

2.149 ± 0.162

2.975 ± 0.163
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski