Kineococcus rhizosphaerae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Kineosporiales; Kineosporiaceae; Kineococcus

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5368 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T0R1T6|A0A2T0R1T6_9ACTN MFS transporter OS=Kineococcus rhizosphaerae OX=559628 GN=CLV37_108145 PE=4 SV=1
MM1 pKa = 7.55TDD3 pKa = 2.67TRR5 pKa = 11.84RR6 pKa = 11.84SYY8 pKa = 10.85EE9 pKa = 3.89GRR11 pKa = 11.84PLPRR15 pKa = 11.84PDD17 pKa = 4.84EE18 pKa = 4.85DD19 pKa = 4.99VDD21 pKa = 3.99DD22 pKa = 4.14QGLAFDD28 pKa = 4.93LTTMLDD34 pKa = 3.01RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84ALGLLGLGAAGLTLAACGSDD57 pKa = 3.45DD58 pKa = 3.52TTAASSTSSSTSATTGTSALTEE80 pKa = 4.42IPDD83 pKa = 3.84EE84 pKa = 4.36TAGPYY89 pKa = 10.2PGDD92 pKa = 3.77GSNGPDD98 pKa = 3.19VLEE101 pKa = 3.84QSGVVRR107 pKa = 11.84SDD109 pKa = 2.62IRR111 pKa = 11.84SSFGSSTTTAEE122 pKa = 4.32GVPMTLTLDD131 pKa = 3.79VVDD134 pKa = 4.52LAGGNVPFEE143 pKa = 4.26GVAVYY148 pKa = 9.93VWHH151 pKa = 7.6CDD153 pKa = 3.18RR154 pKa = 11.84DD155 pKa = 4.18GEE157 pKa = 4.28YY158 pKa = 10.87SLYY161 pKa = 10.99GDD163 pKa = 5.39GITDD167 pKa = 3.36EE168 pKa = 4.33NYY170 pKa = 10.65LRR172 pKa = 11.84GVQIADD178 pKa = 3.23EE179 pKa = 4.89DD180 pKa = 4.39GAVTFTSVYY189 pKa = 7.74PACYY193 pKa = 9.19SGRR196 pKa = 11.84WPHH199 pKa = 5.27VHH201 pKa = 6.5FEE203 pKa = 4.3VYY205 pKa = 9.94PDD207 pKa = 3.36QASITDD213 pKa = 3.62VANCIATSQVALPEE227 pKa = 4.37DD228 pKa = 4.01VSRR231 pKa = 11.84LVYY234 pKa = 9.88ATTGYY239 pKa = 9.0EE240 pKa = 3.82ASVTNLSQVTLATDD254 pKa = 3.44NVFGDD259 pKa = 4.53DD260 pKa = 3.91SGASQTPTATGDD272 pKa = 3.43VTNGYY277 pKa = 10.15ALEE280 pKa = 4.21LTVGVDD286 pKa = 3.34TTTTPTAGTMAGMGSDD302 pKa = 3.67TSGHH306 pKa = 6.45PAGGPGGGTPPTGAAGTAA324 pKa = 3.42

Molecular weight: 33.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T0QYV4|A0A2T0QYV4_9ACTN Iron/manganese superoxide dismutase-like protein (Fragment) OS=Kineococcus rhizosphaerae OX=559628 GN=CLV37_1121 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.45VHH17 pKa = 5.31GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILSARR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.15GRR40 pKa = 11.84AAISAA45 pKa = 3.87

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5368

0

5368

1707126

27

1868

318.0

33.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.289 ± 0.048

0.631 ± 0.007

6.228 ± 0.031

5.171 ± 0.034

2.753 ± 0.02

9.403 ± 0.029

2.235 ± 0.017

2.395 ± 0.025

1.465 ± 0.023

10.781 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.358 ± 0.014

1.537 ± 0.015

5.821 ± 0.028

2.755 ± 0.018

8.187 ± 0.037

5.101 ± 0.025

6.302 ± 0.029

10.388 ± 0.037

1.529 ± 0.014

1.67 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski