Clostridium phage phiCT453A

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A7RTG1|A0A0A7RTG1_9CAUD Uncharacterized protein OS=Clostridium phage phiCT453A OX=1567012 GN=phiCT453A_40 PE=4 SV=1
MM1 pKa = 8.07DD2 pKa = 4.81KK3 pKa = 10.62NYY5 pKa = 10.66EE6 pKa = 4.11IIQDD10 pKa = 3.79ILCRR14 pKa = 11.84AIEE17 pKa = 3.81ITVNQKK23 pKa = 10.15QDD25 pKa = 3.28VSWEE29 pKa = 4.02FFSLSKK35 pKa = 9.81TLSISISFNGEE46 pKa = 3.87CKK48 pKa = 10.08EE49 pKa = 4.16GLVKK53 pKa = 10.03TYY55 pKa = 9.33SVKK58 pKa = 10.58VEE60 pKa = 4.12DD61 pKa = 4.18VEE63 pKa = 4.31CLKK66 pKa = 10.57IIQDD70 pKa = 3.73EE71 pKa = 4.8LINLQYY77 pKa = 11.36EE78 pKa = 4.25NLDD81 pKa = 4.0DD82 pKa = 4.33EE83 pKa = 4.92FLGG86 pKa = 4.38

Molecular weight: 9.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A7RTE7|A0A0A7RTE7_9CAUD Terminase large subunit OS=Clostridium phage phiCT453A OX=1567012 GN=phiCT453A_25 PE=4 SV=1
MM1 pKa = 8.21PDD3 pKa = 4.16RR4 pKa = 11.84IILLPQGRR12 pKa = 11.84IVFIEE17 pKa = 4.15LKK19 pKa = 10.88APGKK23 pKa = 10.01KK24 pKa = 9.31PRR26 pKa = 11.84SIQKK30 pKa = 9.79YY31 pKa = 9.54RR32 pKa = 11.84IKK34 pKa = 10.32EE35 pKa = 3.91LKK37 pKa = 9.81NLGFRR42 pKa = 11.84VEE44 pKa = 4.64IIDD47 pKa = 5.03SIEE50 pKa = 3.85GVNNFIRR57 pKa = 11.84EE58 pKa = 4.0IKK60 pKa = 10.32

Molecular weight: 7.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

62

0

62

12842

40

808

207.1

23.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.875 ± 0.419

0.888 ± 0.133

6.152 ± 0.238

8.441 ± 0.409

4.104 ± 0.201

5.934 ± 0.263

1.106 ± 0.118

8.971 ± 0.364

10.364 ± 0.449

8.231 ± 0.246

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.344 ± 0.143

7.359 ± 0.423

2.562 ± 0.209

2.967 ± 0.173

3.784 ± 0.272

5.35 ± 0.196

5.84 ± 0.286

5.583 ± 0.227

1.106 ± 0.133

4.041 ± 0.211
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski