Kerstersia gyiorum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Alcaligenaceae; Kerstersia

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3267 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A171KTL2|A0A171KTL2_9BURK Alkane 1-monooxygenase OS=Kerstersia gyiorum OX=206506 GN=AAV32_06985 PE=4 SV=1
MM1 pKa = 7.6GSYY4 pKa = 10.48KK5 pKa = 9.29VTPVSGNVLDD15 pKa = 4.53NDD17 pKa = 4.19AAGEE21 pKa = 4.26GAKK24 pKa = 8.75VTHH27 pKa = 7.1IDD29 pKa = 3.8GEE31 pKa = 4.6QVGANGTTTFEE42 pKa = 4.21GEE44 pKa = 4.31YY45 pKa = 11.05GSLTIGANGEE55 pKa = 4.22FTYY58 pKa = 10.5TPYY61 pKa = 9.88DD62 pKa = 3.68TNGLGIGKK70 pKa = 9.16VEE72 pKa = 4.07TFEE75 pKa = 4.11YY76 pKa = 9.61TIKK79 pKa = 10.86AADD82 pKa = 3.92GSEE85 pKa = 4.14STAEE89 pKa = 3.29IHH91 pKa = 6.92IRR93 pKa = 11.84IDD95 pKa = 3.06SDD97 pKa = 3.77GQGLIWPEE105 pKa = 4.6DD106 pKa = 3.56PSQPAEE112 pKa = 3.98VDD114 pKa = 3.71LVANNDD120 pKa = 3.43TGEE123 pKa = 4.25TVINSDD129 pKa = 3.83YY130 pKa = 11.08LVTQGGPSEE139 pKa = 4.09QAPSQTIGGIFAGAVTKK156 pKa = 10.23TSSVNFTVDD165 pKa = 3.47PDD167 pKa = 3.66SKK169 pKa = 11.62ADD171 pKa = 3.67VKK173 pKa = 10.16ITASSPDD180 pKa = 3.6EE181 pKa = 4.25LLVSDD186 pKa = 4.16SLKK189 pKa = 9.83VTVTGPNGYY198 pKa = 10.02SKK200 pKa = 9.65TYY202 pKa = 9.23TGTGGLFSNLSVQEE216 pKa = 3.94MLQGLEE222 pKa = 3.95AGNYY226 pKa = 7.16TVTATYY232 pKa = 10.13QRR234 pKa = 11.84SGTGTGGKK242 pKa = 9.91LEE244 pKa = 4.41LAYY247 pKa = 8.15EE248 pKa = 4.44TQSVTHH254 pKa = 6.59LDD256 pKa = 3.58EE257 pKa = 4.91YY258 pKa = 10.51VVKK261 pKa = 10.58DD262 pKa = 3.26IQAATGNVLADD273 pKa = 3.36NHH275 pKa = 6.83LGSTYY280 pKa = 10.72TKK282 pKa = 10.1FLVEE286 pKa = 4.18NGSGQFVAVANGTTVTGLYY305 pKa = 10.52GVLTINTDD313 pKa = 2.65GSYY316 pKa = 10.71SYY318 pKa = 11.66APTSSLAAIGKK329 pKa = 9.37EE330 pKa = 4.0DD331 pKa = 3.54VFTYY335 pKa = 10.57RR336 pKa = 11.84LEE338 pKa = 4.53HH339 pKa = 6.53PNGTVEE345 pKa = 4.28EE346 pKa = 4.3ATLTIAVEE354 pKa = 4.17HH355 pKa = 5.91GTGPFEE361 pKa = 5.36PDD363 pKa = 3.13ALDD366 pKa = 3.85SADD369 pKa = 4.02EE370 pKa = 4.28FSAFADD376 pKa = 3.25TDD378 pKa = 3.73AFEE381 pKa = 5.89DD382 pKa = 4.2EE383 pKa = 5.24SDD385 pKa = 3.8DD386 pKa = 4.0HH387 pKa = 8.41DD388 pKa = 5.78SDD390 pKa = 4.41VVALRR395 pKa = 11.84GLDD398 pKa = 3.76AEE400 pKa = 5.36DD401 pKa = 3.99GTDD404 pKa = 3.69AEE406 pKa = 4.75PATEE410 pKa = 4.03VAEE413 pKa = 4.7LNLDD417 pKa = 3.82DD418 pKa = 6.02LLYY421 pKa = 10.6EE422 pKa = 4.29PSEE425 pKa = 4.3EE426 pKa = 4.41GDD428 pKa = 3.29VDD430 pKa = 5.22LSALSEE436 pKa = 4.54DD437 pKa = 5.33GEE439 pKa = 4.39DD440 pKa = 3.93QEE442 pKa = 5.51DD443 pKa = 3.67AAAEE447 pKa = 4.01AQEE450 pKa = 4.51SVTAAVEE457 pKa = 3.96TDD459 pKa = 3.62EE460 pKa = 4.78PVVDD464 pKa = 4.08VPLVNPLDD472 pKa = 5.1DD473 pKa = 6.05DD474 pKa = 5.02LNQPQVFLGG483 pKa = 3.63

Molecular weight: 50.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A171KPP8|A0A171KPP8_9BURK RimJ/RimL family protein N-acetyltransferase OS=Kerstersia gyiorum OX=206506 GN=AAV32_14080 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.0RR14 pKa = 11.84THH16 pKa = 5.76GFRR19 pKa = 11.84VRR21 pKa = 11.84MKK23 pKa = 8.99TRR25 pKa = 11.84AGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.89RR41 pKa = 11.84LAVV44 pKa = 3.5

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3267

0

3267

1083391

41

4541

331.6

36.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.465 ± 0.061

0.867 ± 0.013

5.28 ± 0.038

5.245 ± 0.038

3.444 ± 0.028

8.447 ± 0.048

2.182 ± 0.024

4.718 ± 0.03

2.715 ± 0.033

11.225 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.464 ± 0.024

2.734 ± 0.031

5.113 ± 0.03

4.327 ± 0.03

7.002 ± 0.044

5.58 ± 0.031

4.926 ± 0.03

7.281 ± 0.045

1.499 ± 0.017

2.484 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski