Sanxia sobemo-like virus 1

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEW3|A0A1L3KEW3_9VIRU RNA-directed RNA polymerase OS=Sanxia sobemo-like virus 1 OX=1923380 PE=4 SV=1
MM1 pKa = 7.7EE2 pKa = 4.19EE3 pKa = 4.92HH4 pKa = 6.99FDD6 pKa = 3.36QLFYY10 pKa = 11.28HH11 pKa = 6.7CVRR14 pKa = 11.84DD15 pKa = 3.95LTWTSVPGHH24 pKa = 6.71CEE26 pKa = 3.24LAKK29 pKa = 10.89YY30 pKa = 10.48GSTNAQVFDD39 pKa = 4.07LAEE42 pKa = 5.26DD43 pKa = 3.9GTLDD47 pKa = 3.34EE48 pKa = 5.7SRR50 pKa = 11.84FEE52 pKa = 4.0LVKK55 pKa = 10.57QVVYY59 pKa = 10.89LRR61 pKa = 11.84LQVLQDD67 pKa = 3.97KK68 pKa = 10.0PVADD72 pKa = 4.96PIKK75 pKa = 10.72LFIKK79 pKa = 10.54SEE81 pKa = 3.68FHH83 pKa = 6.59KK84 pKa = 10.74RR85 pKa = 11.84EE86 pKa = 4.04KK87 pKa = 10.4ILNSRR92 pKa = 11.84FRR94 pKa = 11.84LISSVSLIDD103 pKa = 3.71GLVDD107 pKa = 3.57RR108 pKa = 11.84MLFIIFAKK116 pKa = 10.52KK117 pKa = 8.89LTGNFANTGICIGYY131 pKa = 8.6NPQKK135 pKa = 10.16GGHH138 pKa = 5.76RR139 pKa = 11.84FMHH142 pKa = 5.53NTFPKK147 pKa = 9.03HH148 pKa = 4.42TEE150 pKa = 3.76KK151 pKa = 11.3LLVDD155 pKa = 3.31QTAFDD160 pKa = 3.72WTYY163 pKa = 10.44KK164 pKa = 10.17PWMANVLKK172 pKa = 10.97NVINDD177 pKa = 3.82LNMDD181 pKa = 4.02PHH183 pKa = 6.1QWWTRR188 pKa = 11.84AVDD191 pKa = 3.66NRR193 pKa = 11.84FKK195 pKa = 11.37ALFDD199 pKa = 3.34KK200 pKa = 10.95PKK202 pKa = 10.46FVFSDD207 pKa = 3.98GEE209 pKa = 4.41RR210 pKa = 11.84IDD212 pKa = 3.82QPIEE216 pKa = 3.68GVMKK220 pKa = 10.19SGCYY224 pKa = 8.44LTIIANSIAVLSIHH238 pKa = 6.0YY239 pKa = 10.08LSSLRR244 pKa = 11.84SNNPTDD250 pKa = 5.23GIILVQGDD258 pKa = 4.49DD259 pKa = 3.88AVQDD263 pKa = 3.6LPLYY267 pKa = 10.23LEE269 pKa = 4.58EE270 pKa = 4.18YY271 pKa = 10.15KK272 pKa = 10.9EE273 pKa = 4.11AVKK276 pKa = 9.79TCGVLPKK283 pKa = 10.4FKK285 pKa = 9.63TSKK288 pKa = 10.31SIEE291 pKa = 4.1FAGFRR296 pKa = 11.84YY297 pKa = 10.37EE298 pKa = 4.18NDD300 pKa = 2.74IFVPEE305 pKa = 4.17YY306 pKa = 9.88RR307 pKa = 11.84QKK309 pKa = 11.01HH310 pKa = 4.66LVALKK315 pKa = 10.41HH316 pKa = 5.36LTSDD320 pKa = 3.46RR321 pKa = 11.84LDD323 pKa = 4.3AEE325 pKa = 4.43MTLNSYY331 pKa = 10.98LRR333 pKa = 11.84MYY335 pKa = 9.86TYY337 pKa = 10.7EE338 pKa = 4.19PTMLAYY344 pKa = 9.5IRR346 pKa = 11.84SLIAKK351 pKa = 9.35RR352 pKa = 11.84NLPKK356 pKa = 10.68AHH358 pKa = 7.0LSDD361 pKa = 4.99RR362 pKa = 11.84QLKK365 pKa = 8.66WFDD368 pKa = 3.11AA369 pKa = 4.21

Molecular weight: 42.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEW3|A0A1L3KEW3_9VIRU RNA-directed RNA polymerase OS=Sanxia sobemo-like virus 1 OX=1923380 PE=4 SV=1
MM1 pKa = 6.07TTKK4 pKa = 10.23VSRR7 pKa = 11.84PDD9 pKa = 3.37QSSSAQNLLNVKK21 pKa = 10.14LEE23 pKa = 4.35SLSPTDD29 pKa = 4.03PPVLQDD35 pKa = 3.54SVIAQTFSSRR45 pKa = 11.84LNMLLMSPVNTILLAVPDD63 pKa = 4.24TDD65 pKa = 3.38TFLIQMISHH74 pKa = 6.28VFSLAILSALFYY86 pKa = 10.16PPTFGALSVWLTASRR101 pKa = 11.84SRR103 pKa = 11.84KK104 pKa = 9.83LMTVCLLLFRR114 pKa = 11.84ALKK117 pKa = 10.14RR118 pKa = 11.84HH119 pKa = 4.59QQVLCRR125 pKa = 11.84LLTTSFTMNTQARR138 pKa = 11.84PFQDD142 pKa = 3.08FLVPRR147 pKa = 11.84TCKK150 pKa = 10.36QIALRR155 pKa = 11.84EE156 pKa = 4.41CILALGPLILAFQSRR171 pKa = 11.84LLICCLIIPIWILMTWIISKK191 pKa = 10.24EE192 pKa = 3.89KK193 pKa = 10.48AISEE197 pKa = 4.06DD198 pKa = 2.93RR199 pKa = 11.84RR200 pKa = 11.84KK201 pKa = 10.39QIEE204 pKa = 4.38RR205 pKa = 11.84LLMIRR210 pKa = 11.84TLTTT214 pKa = 3.13

Molecular weight: 24.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1104

214

521

368.0

41.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.522 ± 0.467

1.359 ± 0.4

5.978 ± 0.652

4.348 ± 0.485

4.438 ± 0.751

4.348 ± 0.951

2.174 ± 0.44

5.616 ± 0.872

5.888 ± 0.706

11.594 ± 1.477

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.08 ± 0.258

3.533 ± 0.437

4.891 ± 0.332

4.438 ± 0.381

5.797 ± 0.315

8.605 ± 1.274

7.246 ± 0.844

5.978 ± 0.199

1.268 ± 0.19

2.899 ± 0.61
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski