Candidatus Vidania fulgoroideae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Betaproteobacteria incertae sedis; Candidatus Vidania

Average proteome isoelectric point is 9.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 154 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A346E0L8|A0A346E0L8_9PROT tRNA-specific 2-thiouridylase MnmA OS=Candidatus Vidania fulgoroideae OX=881286 GN=C9I84_136 PE=4 SV=1
MM1 pKa = 6.82EE2 pKa = 4.81TSFGLYY8 pKa = 9.7IMNNIIGIDD17 pKa = 3.42LGTTNSCVSIMEE29 pKa = 4.39GNTAKK34 pKa = 10.74VIEE37 pKa = 4.28NSEE40 pKa = 4.47GYY42 pKa = 7.92RR43 pKa = 11.84TTPSVVAYY51 pKa = 10.55CNDD54 pKa = 3.91TILVGEE60 pKa = 4.25SAKK63 pKa = 10.53RR64 pKa = 11.84QSLTNPKK71 pKa = 8.55NTIFAVKK78 pKa = 10.02RR79 pKa = 11.84LIGRR83 pKa = 11.84KK84 pKa = 8.15FTDD87 pKa = 3.42PEE89 pKa = 3.79MLEE92 pKa = 4.28NIKK95 pKa = 9.68TSPYY99 pKa = 10.21KK100 pKa = 10.03IVKK103 pKa = 9.91AEE105 pKa = 4.4NGDD108 pKa = 3.13AWVEE112 pKa = 3.83IEE114 pKa = 4.28NGKK117 pKa = 10.25KK118 pKa = 9.42IAPQQVSSEE127 pKa = 4.06ILKK130 pKa = 10.61KK131 pKa = 9.62MKK133 pKa = 9.1KK134 pKa = 7.37TAEE137 pKa = 4.12SYY139 pKa = 10.92LGFEE143 pKa = 4.26IKK145 pKa = 10.25EE146 pKa = 4.06AVITVPAYY154 pKa = 10.64FNDD157 pKa = 3.76SQRR160 pKa = 11.84QATKK164 pKa = 10.34DD165 pKa = 2.98AGMIAGLKK173 pKa = 9.49INRR176 pKa = 11.84IVNEE180 pKa = 3.8PTAAALAFGLDD191 pKa = 3.5KK192 pKa = 11.27NIEE195 pKa = 4.09KK196 pKa = 10.2EE197 pKa = 4.16KK198 pKa = 10.86KK199 pKa = 9.32IVVYY203 pKa = 10.53DD204 pKa = 3.88LGGGTFDD211 pKa = 3.48ISVIEE216 pKa = 4.31ISMIDD221 pKa = 3.46NEE223 pKa = 4.42KK224 pKa = 10.37QFEE227 pKa = 4.31VLSTNGDD234 pKa = 3.7TLLGGEE240 pKa = 4.45DD241 pKa = 4.16FDD243 pKa = 5.27RR244 pKa = 11.84KK245 pKa = 9.96IIKK248 pKa = 9.84FLIDD252 pKa = 3.39KK253 pKa = 9.17YY254 pKa = 11.32LSIEE258 pKa = 4.57GIDD261 pKa = 3.7LEE263 pKa = 4.48KK264 pKa = 10.92DD265 pKa = 2.72ILALQRR271 pKa = 11.84LKK273 pKa = 10.74EE274 pKa = 4.03ASEE277 pKa = 4.1KK278 pKa = 10.74AKK280 pKa = 10.89KK281 pKa = 9.69EE282 pKa = 3.8LSNLEE287 pKa = 3.95STEE290 pKa = 3.65INLPYY295 pKa = 10.24ISINSSGPKK304 pKa = 9.42HH305 pKa = 5.85MKK307 pKa = 9.96ILLTRR312 pKa = 11.84AKK314 pKa = 10.74YY315 pKa = 10.07EE316 pKa = 3.83FLIEE320 pKa = 4.41DD321 pKa = 4.26LVKK324 pKa = 10.03KK325 pKa = 8.57TLIPCEE331 pKa = 3.96TALKK335 pKa = 10.49DD336 pKa = 3.61SLINISDD343 pKa = 3.45IDD345 pKa = 4.57DD346 pKa = 4.6IILVGGMTRR355 pKa = 11.84TPIIQKK361 pKa = 9.68RR362 pKa = 11.84VRR364 pKa = 11.84EE365 pKa = 4.04FFKK368 pKa = 11.01RR369 pKa = 11.84EE370 pKa = 3.63PKK372 pKa = 10.62KK373 pKa = 10.81NINPDD378 pKa = 3.03EE379 pKa = 4.18AVAIGASIQAQILSGDD395 pKa = 3.72RR396 pKa = 11.84KK397 pKa = 10.66DD398 pKa = 4.46VLLLDD403 pKa = 3.87VTPLSLGIEE412 pKa = 4.29TLGGVMTKK420 pKa = 9.89MIVKK424 pKa = 8.49NTTIPTKK431 pKa = 10.22CSQVFSTAEE440 pKa = 3.82EE441 pKa = 4.14NQNSVTIKK449 pKa = 9.68VYY451 pKa = 9.95QGEE454 pKa = 4.15RR455 pKa = 11.84SVAKK459 pKa = 9.57TNKK462 pKa = 10.23LLGEE466 pKa = 4.31FNLEE470 pKa = 4.23EE471 pKa = 4.48IEE473 pKa = 4.05PAPRR477 pKa = 11.84GVPQIEE483 pKa = 4.25VMFDD487 pKa = 2.82IDD489 pKa = 4.69SNGILNVSARR499 pKa = 11.84NKK501 pKa = 8.89VTGKK505 pKa = 9.64EE506 pKa = 3.77NKK508 pKa = 9.46IVIKK512 pKa = 10.67VNSGLTEE519 pKa = 3.9EE520 pKa = 5.2EE521 pKa = 3.92IEE523 pKa = 4.75NMINEE528 pKa = 4.17AKK530 pKa = 10.6KK531 pKa = 10.65NEE533 pKa = 4.14EE534 pKa = 3.66KK535 pKa = 10.99DD536 pKa = 3.62KK537 pKa = 10.64ITLEE541 pKa = 4.28TINLKK546 pKa = 10.62NKK548 pKa = 9.91IEE550 pKa = 3.86NLINQSEE557 pKa = 4.39KK558 pKa = 10.36IIKK561 pKa = 9.86EE562 pKa = 3.79NLNFIEE568 pKa = 4.37KK569 pKa = 10.12EE570 pKa = 4.01NYY572 pKa = 10.18LEE574 pKa = 5.62AEE576 pKa = 4.46TILNEE581 pKa = 4.37NKK583 pKa = 9.78ISYY586 pKa = 9.96LNKK589 pKa = 10.13KK590 pKa = 10.42KK591 pKa = 10.58EE592 pKa = 3.9DD593 pKa = 3.62LEE595 pKa = 4.56LNIINIEE602 pKa = 4.03NVMKK606 pKa = 10.64KK607 pKa = 10.7VNDD610 pKa = 4.54DD611 pKa = 2.99ILRR614 pKa = 11.84NKK616 pKa = 9.4KK617 pKa = 9.92

Molecular weight:
69.06 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A346E0I9|A0A346E0I9_9PROT Uncharacterized protein OS=Candidatus Vidania fulgoroideae OX=881286 GN=C9I84_102 PE=4 SV=1
MM1 pKa = 7.21SRR3 pKa = 11.84VKK5 pKa = 10.61RR6 pKa = 11.84GVFSKK11 pKa = 10.51KK12 pKa = 7.65KK13 pKa = 8.23HH14 pKa = 5.34KK15 pKa = 10.55KK16 pKa = 8.88IIKK19 pKa = 8.27RR20 pKa = 11.84CKK22 pKa = 9.66GFTGRR27 pKa = 11.84RR28 pKa = 11.84KK29 pKa = 9.78NVFRR33 pKa = 11.84ISKK36 pKa = 8.49QAYY39 pKa = 8.26IKK41 pKa = 10.37SLQYY45 pKa = 10.12FYY47 pKa = 11.35RR48 pKa = 11.84DD49 pKa = 3.18TRR51 pKa = 11.84NKK53 pKa = 10.1KK54 pKa = 9.68RR55 pKa = 11.84DD56 pKa = 3.57FRR58 pKa = 11.84KK59 pKa = 9.99LWIININFYY68 pKa = 10.34LRR70 pKa = 11.84KK71 pKa = 10.15YY72 pKa = 8.44NIKK75 pKa = 8.76YY76 pKa = 10.45SRR78 pKa = 11.84FIKK81 pKa = 10.22YY82 pKa = 9.78IKK84 pKa = 9.71EE85 pKa = 3.65LNININRR92 pKa = 11.84KK93 pKa = 8.24SLYY96 pKa = 9.43YY97 pKa = 10.31LCKK100 pKa = 9.62EE101 pKa = 4.06YY102 pKa = 11.36NMKK105 pKa = 10.61KK106 pKa = 10.3LIIYY110 pKa = 10.06INEE113 pKa = 3.94KK114 pKa = 10.59KK115 pKa = 10.73YY116 pKa = 10.83LQNN119 pKa = 3.4

Molecular weight:
15.03 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

154

0

154

42964

37

1292

279.0

33.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

1.543 ± 0.133

1.576 ± 0.055

2.795 ± 0.092

4.19 ± 0.178

7.595 ± 0.277

4.029 ± 0.184

0.968 ± 0.059

14.74 ± 0.187

19.156 ± 0.37

7.8 ± 0.126

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.536 ± 0.063

9.943 ± 0.189

1.769 ± 0.087

1.213 ± 0.063

2.9 ± 0.103

6.28 ± 0.156

3.182 ± 0.091

3.475 ± 0.125

0.314 ± 0.05

4.976 ± 0.131

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski