Clostridium phage phiCT9441A

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 77 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A7RW06|A0A0A7RW06_9CAUD XkdP-like LysM domain-containing protein OS=Clostridium phage phiCT9441A OX=1567014 GN=phiCT9441A_60 PE=4 SV=1
MM1 pKa = 7.56LKK3 pKa = 10.52YY4 pKa = 9.85MKK6 pKa = 10.41DD7 pKa = 3.5GIDD10 pKa = 3.38TYY12 pKa = 11.68FDD14 pKa = 3.69LAVTNEE20 pKa = 4.6DD21 pKa = 3.36PTSNIGKK28 pKa = 7.6QTIVLKK34 pKa = 10.73NCNLDD39 pKa = 3.86EE40 pKa = 4.55VSMAMFDD47 pKa = 3.37VDD49 pKa = 4.88SEE51 pKa = 4.38VLEE54 pKa = 4.33EE55 pKa = 4.78DD56 pKa = 3.13MSFTFEE62 pKa = 5.07DD63 pKa = 3.51VDD65 pKa = 4.57MLDD68 pKa = 3.92KK69 pKa = 10.9FNKK72 pKa = 9.2PVLGG76 pKa = 3.94

Molecular weight: 8.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A7RTR9|A0A0A7RTR9_9CAUD Uncharacterized protein OS=Clostridium phage phiCT9441A OX=1567014 GN=phiCT9441A_11 PE=4 SV=1
MM1 pKa = 7.57KK2 pKa = 9.93IEE4 pKa = 4.97KK5 pKa = 9.52IIKK8 pKa = 8.78AQQPDD13 pKa = 2.52IHH15 pKa = 7.01KK16 pKa = 10.39RR17 pKa = 11.84LKK19 pKa = 8.56QQNRR23 pKa = 11.84KK24 pKa = 9.2KK25 pKa = 9.82KK26 pKa = 8.65SRR28 pKa = 11.84RR29 pKa = 11.84EE30 pKa = 3.95GEE32 pKa = 4.37HH33 pKa = 6.75ISFSDD38 pKa = 3.19VMEE41 pKa = 4.24LMKK44 pKa = 10.41HH45 pKa = 6.35DD46 pKa = 3.34SYY48 pKa = 11.42KK49 pKa = 9.75RR50 pKa = 11.84HH51 pKa = 6.44RR52 pKa = 11.84GAIRR56 pKa = 11.84QRR58 pKa = 3.29

Molecular weight: 7.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

77

0

77

13807

37

667

179.3

20.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.787 ± 0.36

0.84 ± 0.099

5.961 ± 0.244

8.72 ± 0.394

3.831 ± 0.187

5.367 ± 0.255

1.195 ± 0.09

9.343 ± 0.285

11.349 ± 0.366

8.286 ± 0.248

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.564 ± 0.156

7.445 ± 0.237

2.325 ± 0.16

3.114 ± 0.165

3.592 ± 0.185

5.628 ± 0.259

5.179 ± 0.206

5.063 ± 0.222

1.123 ± 0.098

4.288 ± 0.278
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski