Microbacterium phage IAmGroot

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 75 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y6E715|A0A4Y6E715_9CAUD Exonuclease OS=Microbacterium phage IAmGroot OX=2588486 GN=29 PE=4 SV=1
MM1 pKa = 7.39TFYY4 pKa = 10.4TYY6 pKa = 10.89DD7 pKa = 3.37QNNSGGSFDD16 pKa = 3.84VDD18 pKa = 3.56PEE20 pKa = 4.05RR21 pKa = 11.84GIASVVIIEE30 pKa = 4.45ADD32 pKa = 3.38SAEE35 pKa = 4.24EE36 pKa = 3.78ANARR40 pKa = 11.84AEE42 pKa = 4.79DD43 pKa = 3.18IGLYY47 pKa = 10.35FDD49 pKa = 4.97DD50 pKa = 6.27SYY52 pKa = 11.97DD53 pKa = 4.23IDD55 pKa = 4.63CDD57 pKa = 3.86CCGTRR62 pKa = 11.84WSAAWGDD69 pKa = 3.85GDD71 pKa = 5.7DD72 pKa = 3.64VPTVYY77 pKa = 10.33GVEE80 pKa = 4.08RR81 pKa = 11.84SPGDD85 pKa = 3.11SGGRR89 pKa = 11.84GWRR92 pKa = 11.84WTSPEE97 pKa = 3.93GFIHH101 pKa = 6.1YY102 pKa = 10.56ADD104 pKa = 3.97GRR106 pKa = 11.84IEE108 pKa = 5.06AIGVDD113 pKa = 3.6QQ114 pKa = 4.95

Molecular weight: 12.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y6E724|A0A4Y6E724_9CAUD Thioredoxin OS=Microbacterium phage IAmGroot OX=2588486 GN=42 PE=4 SV=1
MM1 pKa = 6.38TTAAPRR7 pKa = 11.84KK8 pKa = 9.0PRR10 pKa = 11.84DD11 pKa = 3.49LGPVHH16 pKa = 7.36LYY18 pKa = 9.85PEE20 pKa = 4.4RR21 pKa = 11.84PKK23 pKa = 11.02ALKK26 pKa = 10.65KK27 pKa = 10.53GDD29 pKa = 3.9NVALAANPDD38 pKa = 3.06DD39 pKa = 3.6WGRR42 pKa = 11.84VQEE45 pKa = 4.15VLPHH49 pKa = 6.63KK50 pKa = 9.56VTEE53 pKa = 4.15PPRR56 pKa = 11.84YY57 pKa = 9.4AVVFVSGVRR66 pKa = 11.84IVQRR70 pKa = 11.84AEE72 pKa = 3.78LIARR76 pKa = 11.84FTTEE80 pKa = 3.73TPRR83 pKa = 11.84KK84 pKa = 9.12KK85 pKa = 10.46GRR87 pKa = 11.84KK88 pKa = 8.26

Molecular weight: 9.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

75

0

75

14095

29

889

187.9

20.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.955 ± 0.491

0.475 ± 0.098

6.25 ± 0.288

6.215 ± 0.262

2.937 ± 0.186

7.691 ± 0.275

1.923 ± 0.134

4.158 ± 0.262

3.469 ± 0.288

9.436 ± 0.457

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.689 ± 0.084

2.114 ± 0.153

5.775 ± 0.292

2.88 ± 0.223

7.272 ± 0.389

5.938 ± 0.447

7.081 ± 0.4

6.939 ± 0.276

1.426 ± 0.157

2.377 ± 0.164
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski