Lysinibacillus sp. F5

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Lysinibacillus; unclassified Lysinibacillus

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4209 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0W7YHE2|A0A0W7YHE2_9BACI Phosphonate metabolism transcriptional regulator PhnF OS=Lysinibacillus sp. F5 OX=1700846 GN=AK833_09350 PE=4 SV=1
MM1 pKa = 6.64THH3 pKa = 5.33EE4 pKa = 4.85HH5 pKa = 4.97NHH7 pKa = 5.31EE8 pKa = 4.27EE9 pKa = 4.15EE10 pKa = 4.27LHH12 pKa = 5.38VQHH15 pKa = 6.38ITVIDD20 pKa = 3.93EE21 pKa = 4.34NGNEE25 pKa = 3.98QLCEE29 pKa = 4.06VIHH32 pKa = 5.08VHH34 pKa = 6.48EE35 pKa = 4.83SPEE38 pKa = 4.03FGKK41 pKa = 10.82SYY43 pKa = 10.82VFYY46 pKa = 11.24SMVGAEE52 pKa = 4.14EE53 pKa = 5.41DD54 pKa = 3.4EE55 pKa = 4.94DD56 pKa = 4.87GSVEE60 pKa = 3.9IFVSSFVPSEE70 pKa = 3.92NGEE73 pKa = 4.24DD74 pKa = 4.2GEE76 pKa = 4.32LTPIEE81 pKa = 4.84TEE83 pKa = 4.35AEE85 pKa = 3.47WDD87 pKa = 3.59MVEE90 pKa = 4.96DD91 pKa = 3.7VLNALEE97 pKa = 5.12DD98 pKa = 4.01EE99 pKa = 4.42NEE101 pKa = 4.09EE102 pKa = 4.16

Molecular weight: 11.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0W7YGV6|A0A0W7YGV6_9BACI Phosphate-starvation-inducible protein PsiE OS=Lysinibacillus sp. F5 OX=1700846 GN=AK833_09945 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.54QPKK8 pKa = 9.44KK9 pKa = 7.96RR10 pKa = 11.84KK11 pKa = 8.69HH12 pKa = 5.94SKK14 pKa = 8.54VHH16 pKa = 5.68GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.19NGRR28 pKa = 11.84KK29 pKa = 8.91VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.81GRR39 pKa = 11.84KK40 pKa = 8.75VLSAA44 pKa = 4.05

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4209

0

4209

1268626

33

2600

301.4

33.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.699 ± 0.042

0.726 ± 0.011

4.952 ± 0.029

7.132 ± 0.039

4.541 ± 0.031

6.484 ± 0.034

2.199 ± 0.017

7.949 ± 0.037

6.335 ± 0.034

9.946 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.879 ± 0.018

4.284 ± 0.03

3.469 ± 0.021

4.434 ± 0.031

3.803 ± 0.028

5.733 ± 0.029

5.812 ± 0.026

7.011 ± 0.03

0.994 ± 0.013

3.619 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski