Raineyella antarctica

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Propionibacteriaceae; Raineyella

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3410 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G6GQ36|A0A1G6GQ36_9ACTN Uncharacterized protein OS=Raineyella antarctica OX=1577474 GN=GA0111570_104240 PE=4 SV=1
MM1 pKa = 7.63TDD3 pKa = 2.56ITTTPPSTGSSEE15 pKa = 4.53DD16 pKa = 3.32LVEE19 pKa = 4.51AYY21 pKa = 9.73FRR23 pKa = 11.84AWNGHH28 pKa = 5.98DD29 pKa = 3.52PSALVAVFSEE39 pKa = 4.23EE40 pKa = 3.65GTYY43 pKa = 10.26QDD45 pKa = 3.89PSLPEE50 pKa = 4.26PVTGDD55 pKa = 4.25AIAGYY60 pKa = 9.71AAALLGVFPDD70 pKa = 4.03LAFSVDD76 pKa = 3.48EE77 pKa = 4.48TTAEE81 pKa = 4.02ADD83 pKa = 3.71RR84 pKa = 11.84TVVAWHH90 pKa = 5.13MTGTNTGPFPGLGGPTGASVDD111 pKa = 3.94LPGIDD116 pKa = 4.35VITVGPGGVTSVVGYY131 pKa = 9.88FDD133 pKa = 3.68QLGLLGQLRR142 pKa = 11.84VPVQIGGAA150 pKa = 3.5

Molecular weight: 15.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G6HUX6|A0A1G6HUX6_9ACTN Chromosome segregation ATPase OS=Raineyella antarctica OX=1577474 GN=GA0111570_112104 PE=4 SV=1
MM1 pKa = 7.62TKK3 pKa = 9.12RR4 pKa = 11.84TYY6 pKa = 10.28QPSNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84ARR15 pKa = 11.84VHH17 pKa = 6.03GFRR20 pKa = 11.84TKK22 pKa = 9.43MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84ATIARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.82GRR40 pKa = 11.84ASLTAA45 pKa = 4.1

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3410

0

3410

1127438

25

3220

330.6

35.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.724 ± 0.057

0.685 ± 0.011

6.125 ± 0.038

5.588 ± 0.044

2.848 ± 0.023

9.136 ± 0.039

2.253 ± 0.021

3.956 ± 0.03

1.992 ± 0.031

10.219 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.125 ± 0.019

1.916 ± 0.021

5.73 ± 0.034

3.094 ± 0.023

7.555 ± 0.046

5.217 ± 0.032

6.135 ± 0.031

9.034 ± 0.039

1.556 ± 0.018

2.112 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski