Streptococcus phage APCM01

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 37 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K0MWL8|A0A0K0MWL8_9CAUD Uncharacterized protein OS=Streptococcus phage APCM01 OX=1647391 GN=APCM01_030 PE=4 SV=1
MM1 pKa = 7.31FKK3 pKa = 10.37ILIGDD8 pKa = 4.21DD9 pKa = 3.78VVCYY13 pKa = 10.21QGNLRR18 pKa = 11.84DD19 pKa = 4.24ALLDD23 pKa = 4.11TIDD26 pKa = 4.55FLTAQKK32 pKa = 10.35HH33 pKa = 4.41EE34 pKa = 4.98CIGAHH39 pKa = 5.68VVLKK43 pKa = 10.59DD44 pKa = 3.58YY45 pKa = 10.9TDD47 pKa = 3.4EE48 pKa = 4.61DD49 pKa = 4.79LIEE52 pKa = 4.98EE53 pKa = 4.34IQSLTDD59 pKa = 3.15SRR61 pKa = 11.84LTISVTLKK69 pKa = 10.66LDD71 pKa = 4.04DD72 pKa = 4.05NVSVVQEE79 pKa = 3.85ILEE82 pKa = 4.09

Molecular weight: 9.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K0MWR6|A0A0K0MWR6_9CAUD Uncharacterized protein OS=Streptococcus phage APCM01 OX=1647391 GN=APCM01_037 PE=4 SV=1
MM1 pKa = 7.33YY2 pKa = 8.72KK3 pKa = 10.03TIKK6 pKa = 10.32KK7 pKa = 9.3KK8 pKa = 10.41ISSIEE13 pKa = 3.83INHH16 pKa = 6.1IVYY19 pKa = 10.78AMSFGEE25 pKa = 3.95AWKK28 pKa = 10.66LKK30 pKa = 9.08VMRR33 pKa = 11.84NKK35 pKa = 10.75DD36 pKa = 3.56YY37 pKa = 11.57VKK39 pKa = 10.4FYY41 pKa = 9.79QTLEE45 pKa = 4.03KK46 pKa = 10.25MLDD49 pKa = 3.22RR50 pKa = 11.84AKK52 pKa = 10.47IDD54 pKa = 3.39VTVEE58 pKa = 3.22NMRR61 pKa = 11.84YY62 pKa = 9.61FNWKK66 pKa = 8.8ICNKK70 pKa = 8.94LGKK73 pKa = 8.98MRR75 pKa = 4.57

Molecular weight: 9.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

37

0

37

9387

50

1718

253.7

28.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.331 ± 0.412

0.575 ± 0.147

6.711 ± 0.439

6.679 ± 0.52

4.378 ± 0.248

5.476 ± 0.691

1.119 ± 0.11

6.307 ± 0.219

9.577 ± 0.599

8.395 ± 0.405

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.971 ± 0.176

5.891 ± 0.259

2.195 ± 0.193

4.283 ± 0.245

4.048 ± 0.285

7.095 ± 0.395

6.498 ± 0.397

5.785 ± 0.36

0.948 ± 0.112

3.739 ± 0.318
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski