Thermogutta terrifontis

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Planctomycetia; Pirellulales; Thermoguttaceae; Thermogutta

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4494 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A286RH97|A0A286RH97_9BACT Uncharacterized protein OS=Thermogutta terrifontis OX=1331910 GN=THTE_2710 PE=4 SV=1
MM1 pKa = 7.21YY2 pKa = 9.45PSDD5 pKa = 5.68DD6 pKa = 4.1SPQTTAPRR14 pKa = 11.84EE15 pKa = 3.97FPITHH20 pKa = 6.83PLVYY24 pKa = 10.05PSDD27 pKa = 5.04DD28 pKa = 3.98SPQTTADD35 pKa = 3.88FRR37 pKa = 11.84PLHH40 pKa = 5.6QNQLVYY46 pKa = 10.5PSDD49 pKa = 5.02DD50 pKa = 3.81SPQTTAFSAAGDD62 pKa = 4.04GEE64 pKa = 4.67KK65 pKa = 9.78IVYY68 pKa = 9.55PSDD71 pKa = 5.06DD72 pKa = 3.87SPQTTAGG79 pKa = 3.41

Molecular weight: 8.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A286REX7|A0A286REX7_9BACT Uncharacterized protein OS=Thermogutta terrifontis OX=1331910 GN=THTE_1902 PE=4 SV=1
MM1 pKa = 7.47ARR3 pKa = 11.84IAQVAGPRR11 pKa = 11.84ASRR14 pKa = 11.84AAFLLRR20 pKa = 11.84RR21 pKa = 11.84SNRR24 pKa = 11.84WKK26 pKa = 10.57LRR28 pKa = 11.84RR29 pKa = 11.84HH30 pKa = 6.29FSNIPPNLHH39 pKa = 5.61TRR41 pKa = 11.84NKK43 pKa = 10.18RR44 pKa = 11.84NRR46 pKa = 11.84TLVWPP51 pKa = 4.69

Molecular weight: 6.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4494

0

4494

1372611

37

6758

305.4

33.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.435 ± 0.039

1.319 ± 0.018

5.012 ± 0.036

6.612 ± 0.045

3.774 ± 0.022

7.792 ± 0.047

2.143 ± 0.018

4.977 ± 0.026

3.484 ± 0.029

10.087 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.945 ± 0.018

2.793 ± 0.034

6.224 ± 0.038

4.037 ± 0.024

7.704 ± 0.039

5.433 ± 0.033

5.144 ± 0.032

7.608 ± 0.035

1.857 ± 0.024

2.62 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski