Streptomyces phage Hank144

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Arquatrovirinae; Arequatrovirus; unclassified Arequatrovirus

Average proteome isoelectric point is 5.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 78 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A385DP02|A0A385DP02_9CAUD Minor tail protein OS=Streptomyces phage Hank144 OX=2301573 GN=20 PE=4 SV=1
MM1 pKa = 7.39SLDD4 pKa = 4.0FDD6 pKa = 4.29TLDD9 pKa = 4.48EE10 pKa = 4.34IAEE13 pKa = 4.19QALTRR18 pKa = 11.84PSDD21 pKa = 3.12AMFWDD26 pKa = 3.91DD27 pKa = 5.82RR28 pKa = 11.84LFTTHH33 pKa = 6.8GAILSWASRR42 pKa = 11.84GDD44 pKa = 4.19DD45 pKa = 2.94ILEE48 pKa = 4.04EE49 pKa = 4.4SNYY52 pKa = 10.35KK53 pKa = 10.23SALDD57 pKa = 4.46LIKK60 pKa = 10.76GAAGDD65 pKa = 3.84DD66 pKa = 3.87AGEE69 pKa = 4.06HH70 pKa = 6.25VIDD73 pKa = 5.18GSAGHH78 pKa = 6.62WLVGTLEE85 pKa = 4.27TIYY88 pKa = 10.65VQVYY92 pKa = 10.55ADD94 pKa = 4.0RR95 pKa = 11.84PEE97 pKa = 4.56CNTIGCEE104 pKa = 4.01EE105 pKa = 4.02EE106 pKa = 4.73AEE108 pKa = 4.12YY109 pKa = 10.47LANYY113 pKa = 7.57RR114 pKa = 11.84TGEE117 pKa = 4.03SAHH120 pKa = 6.04FCEE123 pKa = 4.44EE124 pKa = 4.04HH125 pKa = 7.13KK126 pKa = 10.9EE127 pKa = 3.97GLEE130 pKa = 3.93GSVIAEE136 pKa = 3.82ILHH139 pKa = 6.08TEE141 pKa = 4.26FEE143 pKa = 4.26PLGRR147 pKa = 11.84EE148 pKa = 3.72FTPAFVEE155 pKa = 4.13AAEE158 pKa = 4.5LLVGLQDD165 pKa = 3.62YY166 pKa = 9.33PIIDD170 pKa = 3.52EE171 pKa = 4.36SDD173 pKa = 3.18FSEE176 pKa = 4.83RR177 pKa = 11.84EE178 pKa = 3.69WKK180 pKa = 10.72AFEE183 pKa = 4.97DD184 pKa = 3.86NCSEE188 pKa = 4.12ALEE191 pKa = 4.51SASNEE196 pKa = 3.95YY197 pKa = 10.99DD198 pKa = 3.98DD199 pKa = 5.48DD200 pKa = 4.08TLEE203 pKa = 4.24EE204 pKa = 3.91ATEE207 pKa = 3.78IQNRR211 pKa = 11.84IFQDD215 pKa = 3.29SALSDD220 pKa = 3.57LFGYY224 pKa = 9.39EE225 pKa = 3.97PNAEE229 pKa = 4.21VSWEE233 pKa = 4.01RR234 pKa = 11.84VAEE237 pKa = 4.39IYY239 pKa = 10.82AEE241 pKa = 3.94YY242 pKa = 10.26RR243 pKa = 11.84DD244 pKa = 4.05AYY246 pKa = 10.5FEE248 pKa = 4.01EE249 pKa = 4.66LAYY252 pKa = 9.79EE253 pKa = 4.57AYY255 pKa = 9.58RR256 pKa = 11.84WNVLGYY262 pKa = 10.83NPDD265 pKa = 3.49QLEE268 pKa = 4.11LPIVVVIVAA277 pKa = 4.52

Molecular weight: 31.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A385DR88|A0A385DR88_9CAUD Deoxynucleoside monophosphate OS=Streptomyces phage Hank144 OX=2301573 GN=66 PE=4 SV=1
MM1 pKa = 7.23EE2 pKa = 4.4RR3 pKa = 11.84WIPGRR8 pKa = 11.84EE9 pKa = 3.78GQYY12 pKa = 10.95AITQDD17 pKa = 3.91GVTTSYY23 pKa = 11.23VRR25 pKa = 11.84GKK27 pKa = 10.01PRR29 pKa = 11.84VLKK32 pKa = 10.2WKK34 pKa = 9.86IGTDD38 pKa = 4.18GYY40 pKa = 10.24PRR42 pKa = 11.84VAIAQKK48 pKa = 8.91WVPVHH53 pKa = 5.29TLILEE58 pKa = 4.53AWAGPRR64 pKa = 11.84PDD66 pKa = 3.38GMVVRR71 pKa = 11.84HH72 pKa = 5.93LNGDD76 pKa = 3.4PMKK79 pKa = 10.56PHH81 pKa = 7.5LSQLAWGTQSEE92 pKa = 4.28NLRR95 pKa = 11.84DD96 pKa = 3.79KK97 pKa = 10.64RR98 pKa = 11.84AHH100 pKa = 5.5GTDD103 pKa = 3.0HH104 pKa = 6.64NVNKK108 pKa = 7.47THH110 pKa = 6.63CPKK113 pKa = 10.13GHH115 pKa = 7.12EE116 pKa = 4.09YY117 pKa = 10.66TEE119 pKa = 4.68ANTLRR124 pKa = 11.84RR125 pKa = 11.84ADD127 pKa = 4.11RR128 pKa = 11.84PGHH131 pKa = 5.32RR132 pKa = 11.84ACRR135 pKa = 11.84TCNRR139 pKa = 11.84DD140 pKa = 2.65AVARR144 pKa = 11.84SRR146 pKa = 11.84QRR148 pKa = 11.84KK149 pKa = 6.95RR150 pKa = 11.84QGVAPP155 pKa = 4.45

Molecular weight: 17.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

78

0

78

15511

34

1100

198.9

21.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.921 ± 0.297

0.832 ± 0.122

6.55 ± 0.235

7.485 ± 0.427

2.888 ± 0.19

8.381 ± 0.397

2.018 ± 0.176

4.539 ± 0.32

4.481 ± 0.28

8.278 ± 0.428

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.134 ± 0.13

2.882 ± 0.187

4.861 ± 0.19

3.301 ± 0.149

6.673 ± 0.429

5.983 ± 0.307

6.273 ± 0.245

6.866 ± 0.199

1.825 ± 0.113

2.83 ± 0.216
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski