Guillardia theta (strain CCMP2712) (Cryptophyte)

Taxonomy: cellular organisms; Eukaryota; Cryptophyceae; Pyrenomonadales; Geminigeraceae; Guillardia; Guillardia theta

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 24590 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L1JDQ5|L1JDQ5_GUITC PDZ domain-containing protein OS=Guillardia theta (strain CCMP2712) OX=905079 GN=GUITHDRAFT_107451 PE=4 SV=1
PP1 pKa = 7.02EE2 pKa = 3.79VAGGGDD8 pKa = 3.0WTAAGDD14 pKa = 3.39IWRR17 pKa = 11.84VGCIALHH24 pKa = 6.08LVLGHH29 pKa = 7.28DD30 pKa = 3.89YY31 pKa = 11.38DD32 pKa = 4.03LAGVGSQLPMLPTGVSFDD50 pKa = 3.92FVDD53 pKa = 4.68FLMDD57 pKa = 4.32CMSPDD62 pKa = 3.39PEE64 pKa = 4.1EE65 pKa = 4.2RR66 pKa = 11.84LSAEE70 pKa = 4.03EE71 pKa = 4.08LLEE74 pKa = 4.03HH75 pKa = 7.14AYY77 pKa = 11.35LMDD80 pKa = 4.14EE81 pKa = 4.21VDD83 pKa = 3.4

Molecular weight: 9.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L1K000|L1K000_GUITC Uncharacterized protein OS=Guillardia theta (strain CCMP2712) OX=905079 GN=GUITHDRAFT_150175 PE=4 SV=1
SS1 pKa = 7.43AIGAPHH7 pKa = 6.24TPARR11 pKa = 11.84SHH13 pKa = 6.36ARR15 pKa = 11.84NLVPIPRR22 pKa = 11.84LPSIRR27 pKa = 11.84TAPHH31 pKa = 5.34NPARR35 pKa = 11.84SHH37 pKa = 6.08ARR39 pKa = 11.84NLVHH43 pKa = 7.41IPRR46 pKa = 11.84LPSIRR51 pKa = 11.84TFSYY55 pKa = 9.96TPARR59 pKa = 11.84SHH61 pKa = 6.49ARR63 pKa = 11.84NLVPIPRR70 pKa = 11.84LPSIRR75 pKa = 11.84TAPHH79 pKa = 5.34NPARR83 pKa = 11.84SHH85 pKa = 6.17ARR87 pKa = 11.84NLVPIPRR94 pKa = 11.84LPSIRR99 pKa = 11.84TAPHH103 pKa = 5.09TPARR107 pKa = 11.84SHH109 pKa = 6.36ARR111 pKa = 11.84NLVPIPRR118 pKa = 11.84LPSIRR123 pKa = 11.84TAPHH127 pKa = 5.34NPARR131 pKa = 11.84SHH133 pKa = 6.17ARR135 pKa = 11.84NLVPIPRR142 pKa = 11.84LPSIRR147 pKa = 11.84TAPHH151 pKa = 5.09TPARR155 pKa = 11.84SHH157 pKa = 6.36ARR159 pKa = 11.84NLVPII164 pKa = 4.7

Molecular weight: 18.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

24567

23

24590

10138161

49

14181

412.3

45.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.838 ± 0.022

2.01 ± 0.014

5.456 ± 0.012

7.493 ± 0.031

3.721 ± 0.013

6.693 ± 0.019

2.234 ± 0.009

4.436 ± 0.013

5.521 ± 0.019

9.542 ± 0.021

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.726 ± 0.01

3.496 ± 0.014

4.414 ± 0.016

4.385 ± 0.012

6.32 ± 0.021

8.721 ± 0.023

4.711 ± 0.019

6.566 ± 0.016

1.319 ± 0.005

2.397 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski