Vibrio sp. UCD-FRSSP16_10

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae; Vibrio; unclassified Vibrio

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3062 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1A6KZD8|A0A1A6KZD8_9VIBR Dehydrogenase OS=Vibrio sp. UCD-FRSSP16_10 OX=1853257 GN=A9264_02935 PE=4 SV=1
MM1 pKa = 7.17KK2 pKa = 10.07QIKK5 pKa = 9.72YY6 pKa = 10.33SAIASALLLAGISTSYY22 pKa = 10.44AAEE25 pKa = 4.91PISLTSSGDD34 pKa = 3.57DD35 pKa = 3.38GNAASNTTDD44 pKa = 3.24SDD46 pKa = 3.79LTTRR50 pKa = 11.84WSANGNDD57 pKa = 3.47GSQWVQYY64 pKa = 11.03DD65 pKa = 3.95FGSDD69 pKa = 3.35VPLSGVSIAFYY80 pKa = 10.55KK81 pKa = 10.61GDD83 pKa = 3.43SRR85 pKa = 11.84STYY88 pKa = 10.27FRR90 pKa = 11.84IEE92 pKa = 4.02SSNDD96 pKa = 2.79ASSWSTEE103 pKa = 3.59VDD105 pKa = 3.69SVSSGDD111 pKa = 3.52TADD114 pKa = 3.67QEE116 pKa = 4.89DD117 pKa = 4.25FAFSEE122 pKa = 4.94TVTARR127 pKa = 11.84YY128 pKa = 8.95IRR130 pKa = 11.84YY131 pKa = 9.51VGFGNSSNTWNSVTEE146 pKa = 3.9VDD148 pKa = 4.75FTDD151 pKa = 3.72GDD153 pKa = 4.06EE154 pKa = 4.38NVVVDD159 pKa = 5.27PDD161 pKa = 4.14PVDD164 pKa = 4.26PDD166 pKa = 3.81PVEE169 pKa = 4.52PDD171 pKa = 3.16PVVPVDD177 pKa = 3.79AAALASSDD185 pKa = 4.21DD186 pKa = 3.88GNVADD191 pKa = 5.02NVIDD195 pKa = 3.96SSLDD199 pKa = 3.59TRR201 pKa = 11.84WSANGSGEE209 pKa = 4.11WVRR212 pKa = 11.84LDD214 pKa = 3.9LGSEE218 pKa = 3.88QTLDD222 pKa = 3.31NVQLAFYY229 pKa = 9.42KK230 pKa = 10.64GSSRR234 pKa = 11.84QSIFSIEE241 pKa = 4.1VSDD244 pKa = 6.08DD245 pKa = 3.48DD246 pKa = 5.53SSWTTVLDD254 pKa = 3.54EE255 pKa = 4.78TYY257 pKa = 11.22SSGSSLALEE266 pKa = 4.17TFTFNAVNAQYY277 pKa = 10.64IRR279 pKa = 11.84YY280 pKa = 7.17TGYY283 pKa = 11.46GNTSNTWNSVTEE295 pKa = 4.21FTVSEE300 pKa = 4.31STDD303 pKa = 3.27PGGVVCEE310 pKa = 4.44EE311 pKa = 4.31GSSLVDD317 pKa = 3.64GVCVEE322 pKa = 4.85DD323 pKa = 4.19EE324 pKa = 4.56EE325 pKa = 4.79EE326 pKa = 4.21VTPITCDD333 pKa = 2.93DD334 pKa = 4.36GYY336 pKa = 11.6SLVGDD341 pKa = 3.71EE342 pKa = 4.45CVEE345 pKa = 4.31DD346 pKa = 4.05EE347 pKa = 4.78VVVIPPVTDD356 pKa = 3.56PVDD359 pKa = 3.49GQTYY363 pKa = 7.46YY364 pKa = 11.04TSADD368 pKa = 3.67DD369 pKa = 4.23LNEE372 pKa = 3.98VLASAVGGDD381 pKa = 4.13EE382 pKa = 5.71IIITDD387 pKa = 3.73SGEE390 pKa = 3.8ISIKK394 pKa = 10.56DD395 pKa = 3.27ISFDD399 pKa = 3.61SQVLIRR405 pKa = 11.84AEE407 pKa = 4.2TVGGLKK413 pKa = 10.5LEE415 pKa = 4.43NATIQNSNNITLQGFMFGPSNDD437 pKa = 2.72VSTLVKK443 pKa = 10.14IVNSTNIKK451 pKa = 9.05VLRR454 pKa = 11.84NYY456 pKa = 10.14FDD458 pKa = 4.22HH459 pKa = 7.46LDD461 pKa = 3.57VTEE464 pKa = 4.6GQSSLVVTEE473 pKa = 4.25SSQFIEE479 pKa = 3.32IGYY482 pKa = 10.02NEE484 pKa = 4.13FHH486 pKa = 7.59DD487 pKa = 4.52KK488 pKa = 10.82NISVVDD494 pKa = 3.88GAKK497 pKa = 8.89NTGSYY502 pKa = 10.5IKK504 pKa = 10.24FQYY507 pKa = 10.8DD508 pKa = 2.93VDD510 pKa = 4.02DD511 pKa = 3.97TTGEE515 pKa = 3.96EE516 pKa = 4.97LMTKK520 pKa = 9.93DD521 pKa = 3.34AHH523 pKa = 5.92IHH525 pKa = 5.91HH526 pKa = 7.1NYY528 pKa = 9.37FNNITPYY535 pKa = 10.9LVDD538 pKa = 3.82GTPAGDD544 pKa = 3.67SDD546 pKa = 4.21RR547 pKa = 11.84EE548 pKa = 4.31AIVMGISSSQDD559 pKa = 2.5IEE561 pKa = 4.33TNHH564 pKa = 5.6IVEE567 pKa = 4.36YY568 pKa = 11.12NLFEE572 pKa = 4.5NCDD575 pKa = 3.66GEE577 pKa = 5.24NEE579 pKa = 3.97ILTIKK584 pKa = 9.8TSEE587 pKa = 3.52NTFRR591 pKa = 11.84YY592 pKa = 8.23NTFKK596 pKa = 11.3NSMGSLSFRR605 pKa = 11.84LGYY608 pKa = 10.64NNSAYY613 pKa = 10.56GNYY616 pKa = 9.79FYY618 pKa = 10.76GTGASDD624 pKa = 3.87SVEE627 pKa = 3.97DD628 pKa = 5.33DD629 pKa = 3.39NYY631 pKa = 8.63QTGGIRR637 pKa = 11.84IYY639 pKa = 11.31GEE641 pKa = 3.82GHH643 pKa = 5.42SVYY646 pKa = 10.73DD647 pKa = 3.69NYY649 pKa = 10.73MEE651 pKa = 4.59GLSGTSWRR659 pKa = 11.84LPLLIDD665 pKa = 4.64NGDD668 pKa = 3.74TSDD671 pKa = 3.58SSNGDD676 pKa = 3.2SHH678 pKa = 6.26EE679 pKa = 4.31TPTNINVANNTIVDD693 pKa = 3.93STGGGIYY700 pKa = 9.7IGRR703 pKa = 11.84EE704 pKa = 3.5DD705 pKa = 3.46SSYY708 pKa = 11.63KK709 pKa = 10.41NSPSNITVTGNVVIGSEE726 pKa = 4.2GTLFGNDD733 pKa = 4.04ADD735 pKa = 4.85DD736 pKa = 5.77SSNTWSGNTAYY747 pKa = 10.66NSGSATVNSGGILDD761 pKa = 3.79TSEE764 pKa = 4.67LVEE767 pKa = 4.31LTSSPSVSKK776 pKa = 8.96PTMLTEE782 pKa = 4.05SDD784 pKa = 3.36VGINANN790 pKa = 3.57

Molecular weight: 84.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1A6L279|A0A1A6L279_9VIBR Class II glutamine amidotransferase OS=Vibrio sp. UCD-FRSSP16_10 OX=1853257 GN=A9264_10430 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 8.98RR4 pKa = 11.84TFQPSVLKK12 pKa = 10.49RR13 pKa = 11.84KK14 pKa = 7.97RR15 pKa = 11.84THH17 pKa = 5.89GFRR20 pKa = 11.84ARR22 pKa = 11.84MATKK26 pKa = 10.44NGRR29 pKa = 11.84KK30 pKa = 9.39VINARR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.22GRR40 pKa = 11.84ARR42 pKa = 11.84LSKK45 pKa = 10.83

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3062

0

3062

1010657

36

8186

330.1

36.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.338 ± 0.055

1.011 ± 0.018

5.746 ± 0.079

6.018 ± 0.057

4.118 ± 0.033

6.833 ± 0.053

2.266 ± 0.034

6.651 ± 0.037

5.444 ± 0.056

10.241 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.568 ± 0.029

4.373 ± 0.039

3.756 ± 0.035

4.634 ± 0.043

4.266 ± 0.052

6.898 ± 0.053

5.589 ± 0.066

7.06 ± 0.045

1.183 ± 0.019

3.005 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski