Bacillus sp. LF1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Neobacillus

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5269 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0U1NUE9|A0A0U1NUE9_9BACI Uncharacterized protein OS=Bacillus sp. LF1 OX=1499688 GN=BN000_01552 PE=4 SV=1
MM1 pKa = 7.6KK2 pKa = 10.5SFIVSFHH9 pKa = 6.55QEE11 pKa = 3.98DD12 pKa = 4.28NVDD15 pKa = 3.31SMQIQKK21 pKa = 10.56LNQEE25 pKa = 4.78DD26 pKa = 3.9FDD28 pKa = 4.1KK29 pKa = 11.32AAAGGTRR36 pKa = 11.84HH37 pKa = 6.72LFEE40 pKa = 6.96LDD42 pKa = 3.3TNIGFFVFFDD52 pKa = 4.04AEE54 pKa = 4.2DD55 pKa = 4.02ADD57 pKa = 4.4GVVSYY62 pKa = 10.8LVLQYY67 pKa = 10.66EE68 pKa = 4.6EE69 pKa = 4.72EE70 pKa = 4.35SEE72 pKa = 4.37EE73 pKa = 4.08PSGCYY78 pKa = 9.37SFKK81 pKa = 11.28LEE83 pKa = 4.59DD84 pKa = 4.14FYY86 pKa = 11.63EE87 pKa = 5.11FMALYY92 pKa = 10.86LNDD95 pKa = 5.03LEE97 pKa = 5.65FSDD100 pKa = 3.93EE101 pKa = 4.25TEE103 pKa = 4.49EE104 pKa = 4.05EE105 pKa = 4.21DD106 pKa = 3.99EE107 pKa = 4.52EE108 pKa = 4.57YY109 pKa = 11.47GPIHH113 pKa = 6.63HH114 pKa = 7.18LAHH117 pKa = 6.73LLFHH121 pKa = 6.64IVEE124 pKa = 5.0DD125 pKa = 4.55GNDD128 pKa = 3.39VEE130 pKa = 4.71VV131 pKa = 3.67

Molecular weight: 15.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0U1NSS4|A0A0U1NSS4_9BACI Uncharacterized protein OS=Bacillus sp. LF1 OX=1499688 GN=BN000_00899 PE=4 SV=1
MM1 pKa = 7.48IVFTVKK7 pKa = 10.47EE8 pKa = 3.79IEE10 pKa = 4.37TAVEE14 pKa = 3.93EE15 pKa = 4.57AEE17 pKa = 4.38SLGLNDD23 pKa = 5.99FMYY26 pKa = 10.61QDD28 pKa = 5.16PPSCWGGSSRR38 pKa = 11.84RR39 pKa = 11.84SSRR42 pKa = 11.84RR43 pKa = 11.84SSQSSNNRR51 pKa = 11.84SSSRR55 pKa = 11.84SSQSSNNQSSSRR67 pKa = 11.84SSQSSSNQSSSRR79 pKa = 11.84SSQSSSNQSSSRR91 pKa = 11.84SSQSSSNQSSSRR103 pKa = 11.84SSQSSSRR110 pKa = 11.84SSSRR114 pKa = 11.84RR115 pKa = 11.84ASRR118 pKa = 11.84IARR121 pKa = 11.84RR122 pKa = 11.84RR123 pKa = 11.84SSRR126 pKa = 11.84RR127 pKa = 11.84SSHH130 pKa = 5.48KK131 pKa = 9.57SSRR134 pKa = 11.84RR135 pKa = 11.84SSRR138 pKa = 11.84RR139 pKa = 11.84TRR141 pKa = 11.84FLVIFRR147 pKa = 11.84SQRR150 pKa = 11.84RR151 pKa = 11.84RR152 pKa = 11.84SSQRR156 pKa = 11.84SSKK159 pKa = 10.28RR160 pKa = 11.84SSEE163 pKa = 4.07RR164 pKa = 11.84SSSRR168 pKa = 11.84SRR170 pKa = 11.84ASFSRR175 pKa = 11.84SSRR178 pKa = 11.84RR179 pKa = 11.84SSRR182 pKa = 11.84RR183 pKa = 11.84FRR185 pKa = 11.84RR186 pKa = 11.84RR187 pKa = 11.84SSRR190 pKa = 11.84RR191 pKa = 11.84SSRR194 pKa = 11.84RR195 pKa = 11.84SSRR198 pKa = 11.84RR199 pKa = 11.84SSRR202 pKa = 11.84RR203 pKa = 11.84SSRR206 pKa = 11.84RR207 pKa = 11.84SSRR210 pKa = 11.84RR211 pKa = 11.84SSRR214 pKa = 11.84RR215 pKa = 11.84SSRR218 pKa = 11.84RR219 pKa = 11.84SGRR222 pKa = 11.84RR223 pKa = 11.84SSRR226 pKa = 11.84RR227 pKa = 11.84SRR229 pKa = 11.84RR230 pKa = 11.84RR231 pKa = 3.04

Molecular weight: 26.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5269

0

5269

1494275

29

2891

283.6

31.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.633 ± 0.033

0.809 ± 0.011

4.872 ± 0.028

6.915 ± 0.04

4.801 ± 0.029

6.889 ± 0.036

2.06 ± 0.017

8.185 ± 0.034

7.24 ± 0.033

9.791 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.758 ± 0.016

4.811 ± 0.025

3.701 ± 0.021

3.655 ± 0.022

3.935 ± 0.029

6.135 ± 0.026

5.404 ± 0.029

6.821 ± 0.025

1.079 ± 0.015

3.508 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski