Wabat virus

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B2RVN1|A0A1B2RVN1_9VIRU ORF4 OS=Wabat virus OX=1888308 PE=4 SV=1
MM1 pKa = 7.32FFANNDD7 pKa = 3.53QSCMRR12 pKa = 11.84TTQRR16 pKa = 11.84RR17 pKa = 11.84QSWRR21 pKa = 11.84GDD23 pKa = 3.23SKK25 pKa = 10.8QIRR28 pKa = 11.84SNLMLRR34 pKa = 11.84GSNPSTSPKK43 pKa = 10.07VSLGAQVSTTQLKK56 pKa = 8.21PQRR59 pKa = 11.84FKK61 pKa = 9.68FQKK64 pKa = 7.67MAFYY68 pKa = 10.42IPPEE72 pKa = 3.83FDD74 pKa = 2.96YY75 pKa = 11.68DD76 pKa = 3.52EE77 pKa = 4.73AKK79 pKa = 10.43RR80 pKa = 11.84EE81 pKa = 4.03RR82 pKa = 11.84EE83 pKa = 4.06LFLCKK88 pKa = 9.49NTFIQYY94 pKa = 8.67CTMGHH99 pKa = 5.94HH100 pKa = 6.58AVYY103 pKa = 10.21AYY105 pKa = 9.56IPYY108 pKa = 10.21VADD111 pKa = 3.8RR112 pKa = 11.84VDD114 pKa = 3.91KK115 pKa = 11.02EE116 pKa = 4.69HH117 pKa = 7.56IDD119 pKa = 3.36PLRR122 pKa = 11.84NFFPEE127 pKa = 4.5MIQLIEE133 pKa = 4.31RR134 pKa = 11.84SDD136 pKa = 3.67MEE138 pKa = 4.37VVRR141 pKa = 11.84DD142 pKa = 3.88TFKK145 pKa = 11.11LKK147 pKa = 9.91QGHH150 pKa = 6.4MINILSKK157 pKa = 10.77ARR159 pKa = 11.84SCMEE163 pKa = 3.7KK164 pKa = 10.32QLADD168 pKa = 4.05NLRR171 pKa = 11.84KK172 pKa = 9.74IYY174 pKa = 10.72VYY176 pKa = 10.6LARR179 pKa = 11.84DD180 pKa = 4.11LLDD183 pKa = 3.96QLQPWFVNRR192 pKa = 11.84AMEE195 pKa = 4.5TFDD198 pKa = 4.44EE199 pKa = 4.34DD200 pKa = 3.54HH201 pKa = 6.82LRR203 pKa = 11.84PTTVPPPYY211 pKa = 9.87QEE213 pKa = 5.05GGALPSYY220 pKa = 10.29EE221 pKa = 4.82SISEE225 pKa = 4.23VPQTRR230 pKa = 11.84DD231 pKa = 2.49SCTQIEE237 pKa = 4.32AHH239 pKa = 7.42DD240 pKa = 3.84MQQWEE245 pKa = 4.7DD246 pKa = 3.61FLNPRR251 pKa = 11.84DD252 pKa = 3.98EE253 pKa = 4.57ILLGDD258 pKa = 4.23HH259 pKa = 7.12DD260 pKa = 5.96GIWAHH265 pKa = 6.34YY266 pKa = 7.01EE267 pKa = 3.95QQQGEE272 pKa = 4.43LWIDD276 pKa = 3.58ATPDD280 pKa = 4.24FYY282 pKa = 11.0PEE284 pKa = 4.06TPTRR288 pKa = 11.84AIWEE292 pKa = 4.11NFRR295 pKa = 11.84TVNNLRR301 pKa = 11.84DD302 pKa = 3.9APPEE306 pKa = 3.85YY307 pKa = 10.15RR308 pKa = 11.84DD309 pKa = 3.39VLLFILTEE317 pKa = 4.1QYY319 pKa = 10.81HH320 pKa = 6.62PEE322 pKa = 3.77LTLAQDD328 pKa = 4.06YY329 pKa = 9.55EE330 pKa = 4.42HH331 pKa = 7.4TDD333 pKa = 2.98TDD335 pKa = 4.31EE336 pKa = 3.89IRR338 pKa = 11.84SIARR342 pKa = 11.84RR343 pKa = 11.84CVMWHH348 pKa = 5.55YY349 pKa = 10.36RR350 pKa = 11.84DD351 pKa = 4.97CGFCMLGLVHH361 pKa = 5.94CHH363 pKa = 6.08CRR365 pKa = 11.84ANCNCNDD372 pKa = 4.48RR373 pKa = 11.84SCVWNWLEE381 pKa = 4.83ADD383 pKa = 3.26INSYY387 pKa = 8.4LTAPNGMFYY396 pKa = 10.52EE397 pKa = 4.23LRR399 pKa = 11.84SLVMYY404 pKa = 10.11IEE406 pKa = 4.2IMGAQEE412 pKa = 5.7QNDD415 pKa = 3.22NWDD418 pKa = 3.22RR419 pKa = 11.84WFDD422 pKa = 3.79SYY424 pKa = 11.92VGATVAGHH432 pKa = 5.39SCFWSDD438 pKa = 2.78AFEE441 pKa = 3.95WTRR444 pKa = 11.84RR445 pKa = 11.84SMSS448 pKa = 3.06

Molecular weight: 52.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B2RVM9|A0A1B2RVM9_9VIRU Capsid protein OS=Wabat virus OX=1888308 PE=4 SV=1
MM1 pKa = 7.03QAIAGLAGALPNTLGMGLDD20 pKa = 3.59TMNRR24 pKa = 11.84ALDD27 pKa = 3.84RR28 pKa = 11.84NQQNSQFRR36 pKa = 11.84ARR38 pKa = 11.84EE39 pKa = 3.8EE40 pKa = 4.22AFTKK44 pKa = 10.62AGLPAFLAYY53 pKa = 9.37TGAAGQPYY61 pKa = 9.49YY62 pKa = 9.95PSSYY66 pKa = 10.48SNVGGRR72 pKa = 11.84TNYY75 pKa = 7.81RR76 pKa = 11.84TGMVGSPYY84 pKa = 9.86TGQSNSLFTNAEE96 pKa = 3.95AVTNTSLGSSSPSGEE111 pKa = 4.19GPPQPMTTPTYY122 pKa = 10.15TSISSTRR129 pKa = 11.84SGFNRR134 pKa = 11.84MQGPRR139 pKa = 11.84FLKK142 pKa = 10.58DD143 pKa = 2.92VLRR146 pKa = 11.84EE147 pKa = 4.0QRR149 pKa = 11.84PEE151 pKa = 3.94LYY153 pKa = 10.49EE154 pKa = 4.24NNATQAEE161 pKa = 4.61LAWGLKK167 pKa = 9.83ADD169 pKa = 5.58PIQPHH174 pKa = 5.7VKK176 pKa = 10.24RR177 pKa = 11.84KK178 pKa = 9.43

Molecular weight: 19.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

3740

178

2584

935.0

106.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.497 ± 0.528

1.925 ± 0.584

6.07 ± 0.482

6.684 ± 0.68

4.519 ± 0.168

5.428 ± 0.7

3.048 ± 0.314

5.668 ± 0.615

6.364 ± 1.282

7.701 ± 0.658

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.021 ± 0.44

4.626 ± 0.392

5.187 ± 0.412

3.556 ± 0.793

5.16 ± 0.618

6.898 ± 0.716

6.203 ± 0.349

6.283 ± 0.787

1.551 ± 0.329

3.61 ± 0.393
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski