Amphritea opalescens

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Oceanospirillaceae; Amphritea

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3573 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A430KTX0|A0A430KTX0_9GAMM EF-P post-translational modification enzyme B OS=Amphritea opalescens OX=2490544 GN=epmB PE=3 SV=1
MM1 pKa = 7.67RR2 pKa = 11.84KK3 pKa = 8.68SHH5 pKa = 6.87SGFTLIEE12 pKa = 3.82ILIAIAVVGVLAAIAIPAYY31 pKa = 9.53TSYY34 pKa = 8.03TTKK37 pKa = 10.94AKK39 pKa = 10.19VSEE42 pKa = 4.34GFSIVSPVKK51 pKa = 10.58LRR53 pKa = 11.84MADD56 pKa = 3.35EE57 pKa = 4.38LMQTGEE63 pKa = 4.27IPATDD68 pKa = 3.19TGLGLNLGDD77 pKa = 4.69YY78 pKa = 7.4EE79 pKa = 4.34TDD81 pKa = 3.72YY82 pKa = 11.38IDD84 pKa = 3.61TVSVSGGVISVEE96 pKa = 4.07YY97 pKa = 10.57NLSKK101 pKa = 10.87LGNHH105 pKa = 6.05NVLQFVPRR113 pKa = 11.84CNNNYY118 pKa = 10.35LIGWDD123 pKa = 3.76CTSPDD128 pKa = 3.36GSGVSAEE135 pKa = 4.18YY136 pKa = 10.79LPTSCASGNGSASLTCTLASVVSTSGSTGSGSTGSTGSTGSTGSTGSTGDD186 pKa = 3.49TGSTDD191 pKa = 3.6ANGDD195 pKa = 3.52NGDD198 pKa = 4.06NGDD201 pKa = 4.29NGDD204 pKa = 4.29NGDD207 pKa = 4.29NGDD210 pKa = 4.29NGDD213 pKa = 4.29NGDD216 pKa = 4.29NGDD219 pKa = 4.29NGDD222 pKa = 4.29NGDD225 pKa = 4.29NGDD228 pKa = 4.29NGDD231 pKa = 4.29NGDD234 pKa = 4.29NGDD237 pKa = 4.29NGDD240 pKa = 4.29NGDD243 pKa = 4.62NGDD246 pKa = 4.16NGNNGNNGNNGNNGNNGNNGNNGNNGNNGNNGNNGNNGNNGNNGNNGNNGNNGNNGNNGNNGNNAAQCSNKK317 pKa = 9.52GRR319 pKa = 11.84DD320 pKa = 3.19SCIQSQSCQWQGGRR334 pKa = 11.84RR335 pKa = 11.84SGSCVSII342 pKa = 4.2

Molecular weight: 34.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A430KPL0|A0A430KPL0_9GAMM Glutamine--fructose-6-phosphate aminotransferase [isomerizing] OS=Amphritea opalescens OX=2490544 GN=glmS PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 10.2RR12 pKa = 11.84KK13 pKa = 7.57RR14 pKa = 11.84THH16 pKa = 5.9GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.08SGRR28 pKa = 11.84VIINRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.74GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3573

0

3573

1184853

23

12600

331.6

36.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.209 ± 0.042

1.055 ± 0.015

5.733 ± 0.056

6.115 ± 0.036

3.828 ± 0.026

7.221 ± 0.046

2.219 ± 0.021

6.24 ± 0.034

4.384 ± 0.035

10.902 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.691 ± 0.026

3.714 ± 0.034

4.204 ± 0.029

4.764 ± 0.045

5.115 ± 0.044

6.383 ± 0.038

5.298 ± 0.05

6.872 ± 0.039

1.216 ± 0.016

2.837 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski