Desulfotomaculum aquiferis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Peptococcaceae; Desulfotomaculum

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6019 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q2WFS3|A0A4Q2WFS3_9FIRM Biotin_lipoyl_2 domain-containing protein OS=Desulfotomaculum aquiferis OX=1397668 GN=N752_27115 PE=4 SV=1
MM1 pKa = 7.0KK2 pKa = 9.99TVVDD6 pKa = 3.62QDD8 pKa = 3.93LCISCGACIDD18 pKa = 3.82VCPEE22 pKa = 3.67VYY24 pKa = 9.55EE25 pKa = 4.43WNDD28 pKa = 3.17DD29 pKa = 4.01DD30 pKa = 4.22KK31 pKa = 12.08AHH33 pKa = 7.05AIVDD37 pKa = 4.11EE38 pKa = 4.43VPEE41 pKa = 4.32GQEE44 pKa = 4.34DD45 pKa = 4.06SAKK48 pKa = 10.12EE49 pKa = 3.78AAEE52 pKa = 4.16SCPTEE57 pKa = 4.41AIKK60 pKa = 11.22VNN62 pKa = 3.6

Molecular weight: 6.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q2WQW4|A0A4Q2WQW4_9FIRM Uncharacterized protein OS=Desulfotomaculum aquiferis OX=1397668 GN=N752_04875 PE=4 SV=1
MM1 pKa = 7.4GVPKK5 pKa = 10.35RR6 pKa = 11.84RR7 pKa = 11.84LAKK10 pKa = 9.95HH11 pKa = 6.54RR12 pKa = 11.84GRR14 pKa = 11.84QRR16 pKa = 11.84RR17 pKa = 11.84AMNMKK22 pKa = 10.37LEE24 pKa = 4.35APSLVACPQCRR35 pKa = 11.84ALIQPHH41 pKa = 6.21HH42 pKa = 6.91LCPEE46 pKa = 3.99CGYY49 pKa = 9.85YY50 pKa = 10.39KK51 pKa = 10.49NRR53 pKa = 11.84EE54 pKa = 3.7VVAATKK60 pKa = 10.47

Molecular weight: 6.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6019

0

6019

955997

29

998

158.8

17.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.485 ± 0.04

1.216 ± 0.019

4.639 ± 0.028

6.685 ± 0.035

3.946 ± 0.023

7.446 ± 0.041

1.734 ± 0.016

7.784 ± 0.03

6.303 ± 0.031

10.392 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.976 ± 0.017

4.421 ± 0.03

4.122 ± 0.028

3.693 ± 0.025

4.76 ± 0.029

5.774 ± 0.026

5.083 ± 0.03

7.256 ± 0.033

1.062 ± 0.015

3.218 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski