uncultured phage_MedDCM-OCT-S28-C3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Pedosvirus; Pedosvirus S28C3

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6S4PLC9|A0A6S4PLC9_9CAUD Uncharacterized protein OS=uncultured phage_MedDCM-OCT-S28-C3 OX=2740802 PE=4 SV=1
MM1 pKa = 6.77NTATYY6 pKa = 8.6TLPTHH11 pKa = 6.53WASALINGDD20 pKa = 2.81WSGLDD25 pKa = 3.53EE26 pKa = 4.38QDD28 pKa = 3.48EE29 pKa = 4.45EE30 pKa = 4.4ALTRR34 pKa = 11.84VMHH37 pKa = 6.86GEE39 pKa = 4.07ALPDD43 pKa = 3.93CLDD46 pKa = 3.38VRR48 pKa = 11.84DD49 pKa = 4.57DD50 pKa = 3.62STFRR54 pKa = 11.84KK55 pKa = 9.67YY56 pKa = 10.62HH57 pKa = 6.42DD58 pKa = 3.82AQPYY62 pKa = 9.48GVLACDD68 pKa = 3.92CSTFLFPGNN77 pKa = 3.6

Molecular weight: 8.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6S4PDC3|A0A6S4PDC3_9CAUD DNA polymerase I (TIGR00593) OS=uncultured phage_MedDCM-OCT-S28-C3 OX=2740802 PE=4 SV=1
MM1 pKa = 7.0SQIEE5 pKa = 3.92VRR7 pKa = 11.84QRR9 pKa = 11.84VRR11 pKa = 11.84EE12 pKa = 3.9QQAKK16 pKa = 9.3QKK18 pKa = 10.59EE19 pKa = 4.3IVLKK23 pKa = 10.07YY24 pKa = 10.24RR25 pKa = 11.84GVAYY29 pKa = 9.52IVKK32 pKa = 10.31RR33 pKa = 11.84NVASNN38 pKa = 3.24

Molecular weight: 4.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

12897

38

1328

263.2

29.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.979 ± 0.481

0.915 ± 0.192

6.637 ± 0.32

6.118 ± 0.402

3.567 ± 0.205

6.606 ± 0.45

1.636 ± 0.218

5.296 ± 0.22

5.722 ± 0.421

7.901 ± 0.37

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.621 ± 0.249

4.676 ± 0.358

4.109 ± 0.292

5.024 ± 0.501

5.505 ± 0.455

6.73 ± 0.512

7.614 ± 0.7

6.079 ± 0.294

1.062 ± 0.145

3.202 ± 0.154
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski