Escherichia phage HK022 (Bacteriophage HK022)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Hendrixvirinae; Shamshuipovirus

Average proteome isoelectric point is 7.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 69 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2I6PIG6|A0A2I6PIG6_BPHK0 KilA-N domain-containing protein OS=Escherichia phage HK022 OX=10742 GN=HK022_73 PE=4 SV=1
MM1 pKa = 7.76IDD3 pKa = 3.19QNRR6 pKa = 11.84SYY8 pKa = 10.32EE9 pKa = 4.03QEE11 pKa = 4.1SVEE14 pKa = 5.1RR15 pKa = 11.84ALTCANCGQKK25 pKa = 10.32LHH27 pKa = 5.86VLEE30 pKa = 4.31VHH32 pKa = 5.94VCEE35 pKa = 4.54HH36 pKa = 6.71CCAEE40 pKa = 4.99LMSDD44 pKa = 4.35PNSSMYY50 pKa = 10.6EE51 pKa = 3.98EE52 pKa = 5.0EE53 pKa = 4.99DD54 pKa = 4.39DD55 pKa = 4.1EE56 pKa = 4.75

Molecular weight: 6.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9MCV3|Q9MCV3_BPHK0 Uncharacterized protein OS=Escherichia phage HK022 OX=10742 GN=HK022_8 PE=4 SV=1
MM1 pKa = 7.02QAGKK5 pKa = 9.89LRR7 pKa = 11.84HH8 pKa = 6.56RR9 pKa = 11.84ITLQEE14 pKa = 3.91PVKK17 pKa = 10.52VQNPTTGAVINTWRR31 pKa = 11.84DD32 pKa = 3.18VATVRR37 pKa = 11.84AEE39 pKa = 3.93VSPLSARR46 pKa = 11.84EE47 pKa = 3.97FIAAQASQGEE57 pKa = 4.39ITTRR61 pKa = 11.84IVIRR65 pKa = 11.84YY66 pKa = 7.05RR67 pKa = 11.84AGVTRR72 pKa = 11.84KK73 pKa = 9.73HH74 pKa = 6.46RR75 pKa = 11.84ILFRR79 pKa = 11.84GAVYY83 pKa = 10.34NIHH86 pKa = 6.74GVLPDD91 pKa = 3.77PKK93 pKa = 10.36SGRR96 pKa = 11.84EE97 pKa = 3.98YY98 pKa = 10.53LTLPCSEE105 pKa = 4.45GVNDD109 pKa = 4.16GG110 pKa = 3.76

Molecular weight: 12.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

69

0

69

12414

34

1183

179.9

19.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.32 ± 0.362

1.079 ± 0.14

5.969 ± 0.22

6.017 ± 0.273

3.319 ± 0.188

7.58 ± 0.345

1.611 ± 0.206

6.227 ± 0.197

5.897 ± 0.316

7.741 ± 0.308

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.481 ± 0.161

4.608 ± 0.235

3.834 ± 0.205

4.43 ± 0.251

6.404 ± 0.348

6.767 ± 0.29

5.792 ± 0.313

6.235 ± 0.256

1.635 ± 0.113

3.053 ± 0.202
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski