African pouched rat arterivirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Arnidovirineae; Arteriviridae; Heroarterivirinae; Lambdaarterivirus; Lambdaarterivirus afriporav

Average proteome isoelectric point is 7.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0B5JFS1|A0A0B5JFS1_9NIDO Nucleocapsid protein OS=African pouched rat arterivirus OX=1965064 GN=ORF7 PE=3 SV=1
MM1 pKa = 7.44GANIPSVHH9 pKa = 6.67LLIMCCCLHH18 pKa = 6.63AVSGDD23 pKa = 3.48DD24 pKa = 3.82TEE26 pKa = 4.96NSLVSCFSFPEE37 pKa = 4.28SNKK40 pKa = 10.3GSLQLSATVSTCPAKK55 pKa = 10.56YY56 pKa = 10.26LRR58 pKa = 11.84ATTGEE63 pKa = 4.14EE64 pKa = 4.01GAKK67 pKa = 10.73ANGVNAKK74 pKa = 10.18AKK76 pKa = 9.44GGFYY80 pKa = 10.68VSVNPKK86 pKa = 10.17HH87 pKa = 5.92PWCGYY92 pKa = 8.8HH93 pKa = 6.84HH94 pKa = 7.04AVDD97 pKa = 3.97NPGTEE102 pKa = 3.86YY103 pKa = 10.72QYY105 pKa = 11.48RR106 pKa = 11.84DD107 pKa = 3.33TTLCGAIMLVGYY119 pKa = 9.62YY120 pKa = 10.35DD121 pKa = 3.22HH122 pKa = 7.42VYY124 pKa = 10.98NFVCTGHH131 pKa = 6.13HH132 pKa = 5.48TANNSFTYY140 pKa = 9.6EE141 pKa = 4.21LPPHH145 pKa = 6.15QIVLPMVLQVATYY158 pKa = 10.19VAILHH163 pKa = 5.84PQLFGFEE170 pKa = 4.56NITAVVAHH178 pKa = 6.5EE179 pKa = 3.94NFICFEE185 pKa = 4.38HH186 pKa = 6.81GGNPVNITKK195 pKa = 10.06AHH197 pKa = 6.85PDD199 pKa = 2.96SGFRR203 pKa = 11.84STLYY207 pKa = 10.55DD208 pKa = 5.71LEE210 pKa = 4.61VQWQYY215 pKa = 11.81LEE217 pKa = 3.93WVRR220 pKa = 11.84PLFSCWLVMCISLTLRR236 pKa = 11.84RR237 pKa = 11.84SSALLAEE244 pKa = 4.58RR245 pKa = 4.66

Molecular weight:
27.14 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A0B5JKI0|A0A0B5JKI0_9NIDO GP4 glycoprotein OS=African pouched rat arterivirus OX=1965064 GN=ORF4 PE=4 SV=1
MM1 pKa = 7.58SGPIGFSHH9 pKa = 7.51ALFDD13 pKa = 3.66QLTRR17 pKa = 11.84AGMPRR22 pKa = 11.84RR23 pKa = 11.84KK24 pKa = 9.35AHH26 pKa = 6.88KK27 pKa = 9.61NPQPAHH33 pKa = 5.84QDD35 pKa = 3.28LVKK38 pKa = 10.81ALSSLLNKK46 pKa = 10.05GQGPKK51 pKa = 9.98KK52 pKa = 9.64GQAGSGSRR60 pKa = 11.84QKK62 pKa = 10.54RR63 pKa = 11.84HH64 pKa = 5.53EE65 pKa = 4.22NALQYY70 pKa = 10.89PLAQPGDD77 pKa = 4.15LRR79 pKa = 11.84RR80 pKa = 11.84MLKK83 pKa = 8.6PTSAPMVRR91 pKa = 11.84SALLSAFNSGGGILVGDD108 pKa = 4.29ADD110 pKa = 3.97GKK112 pKa = 9.39VTFTCDD118 pKa = 2.66IQVPPRR124 pKa = 11.84FIHH127 pKa = 6.12QPKK130 pKa = 9.62QPSEE134 pKa = 3.9

Molecular weight:
14.43 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

1

10

7352

59

3714

735.2

80.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.399 ± 0.398

3.509 ± 0.251

4.312 ± 0.596

4.067 ± 0.315

3.904 ± 0.548

7.331 ± 0.436

2.979 ± 0.386

3.808 ± 0.309

3.904 ± 0.249

10.365 ± 0.488

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.823 ± 0.381

3.346 ± 0.28

6.27 ± 0.806

2.924 ± 0.293

5.169 ± 0.283

7.141 ± 0.485

5.794 ± 0.273

9.168 ± 0.539

1.469 ± 0.141

3.319 ± 0.312

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski