Proteome-pI 2.0 - Proteome Isoelectric Point Database

Caldanaerobius fijiensis DSM 17918

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Thermoanaerobacterales; Thermoanaerobacteraceae; Caldanaerobius; Caldanaerobius fijiensis

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 2707 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|A0A1M5BX91|A0A1M5BX91_9THEO 2-C-methyl-D-erythritol 2 4-cyclodiphosphate synthase OS=Caldanaerobius fijiensis DSM 17918 OX=1121256 GN=ispF PE=3 SV=1

MM1 pKa = 7.55KK2 pKa = 10.16KK3 pKa = 8.65WQCTVCGYY11 pKa = 10.27IYY13 pKa = 10.56DD14 pKa = 4.2PAKK17 pKa = 10.48GDD19 pKa = 4.03PDD21 pKa = 3.73NGIAPGTAFEE31 pKa = 5.32DD32 pKa = 5.04LPDD35 pKa = 4.01DD36 pKa = 4.49WVCPDD41 pKa = 4.09CGVGKK46 pKa = 9.95DD47 pKa = 3.44QFEE50 pKa = 4.6EE51 pKa = 3.83MDD53 pKa = 3.61EE54 pKa = 4.08

Molecular weight: 6.0 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|A0A1M4YSE5|A0A1M4YSE5_9THEO Translation initiation factor IF-1 OS=Caldanaerobius fijiensis DSM 17918 OX=1121256 GN=infA PE=3 SV=1

MM1 pKa = 6.97YY2 pKa = 9.77HH3 pKa = 6.76RR4 pKa = 11.84KK5 pKa = 9.26LRR7 pKa = 11.84RR8 pKa = 11.84PTDD11 pKa = 2.98QRR13 pKa = 11.84MAMLRR18 pKa = 11.84NLTTSLLKK26 pKa = 10.36HH27 pKa = 5.77GRR29 pKa = 11.84IKK31 pKa = 8.25TTEE34 pKa = 3.63ARR36 pKa = 11.84AKK38 pKa = 6.86EE39 pKa = 4.12TRR41 pKa = 11.84KK42 pKa = 9.4FAEE45 pKa = 4.49KK46 pKa = 9.97MITLAKK52 pKa = 10.41RR53 pKa = 11.84GDD55 pKa = 3.38LHH57 pKa = 7.45ARR59 pKa = 11.84RR60 pKa = 11.84QALAFIYY67 pKa = 10.7DD68 pKa = 3.61EE69 pKa = 4.4TVVKK73 pKa = 10.91NLFDD77 pKa = 5.01EE78 pKa = 4.56IAPRR82 pKa = 11.84YY83 pKa = 9.85ADD85 pKa = 3.61RR86 pKa = 11.84QGGYY90 pKa = 7.28TRR92 pKa = 11.84IIKK95 pKa = 10.02IGPRR99 pKa = 11.84RR100 pKa = 11.84GDD102 pKa = 3.69AAPMAIIEE110 pKa = 4.78LII112 pKa = 3.72

Molecular weight: 13.02 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
2707	0	2707	825341	28	2892	304.9	34.32

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
6.697 ± 0.046	1.039 ± 0.019	5.924 ± 0.032	6.712 ± 0.052	4.027 ± 0.034	7.017 ± 0.04	1.56 ± 0.018	9.168 ± 0.048	7.717 ± 0.042	9.068 ± 0.045

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.869 ± 0.022	4.846 ± 0.039	3.595 ± 0.027	2.725 ± 0.028	4.484 ± 0.034	5.556 ± 0.037	4.591 ± 0.032	7.226 ± 0.043	0.919 ± 0.018	4.258 ± 0.033

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski