Bacteroidaceae bacterium HV4-6-C5C

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae; unclassified Bacteroidaceae

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2881 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C6HKY2|A0A5C6HKY2_9BACT Uncharacterized protein OS=Bacteroidaceae bacterium HV4-6-C5C OX=2600309 GN=FQ707_11000 PE=4 SV=1
MM1 pKa = 7.54NYY3 pKa = 10.87DD4 pKa = 3.06NFLRR8 pKa = 11.84FIFPDD13 pKa = 3.18GMFDD17 pKa = 3.51YY18 pKa = 11.48FEE20 pKa = 3.94MVGFNEE26 pKa = 4.63LSDD29 pKa = 3.47KK30 pKa = 11.19VEE32 pKa = 4.17IYY34 pKa = 10.74FEE36 pKa = 4.26EE37 pKa = 4.85KK38 pKa = 9.21NTLSEE43 pKa = 4.67EE44 pKa = 4.21YY45 pKa = 10.87DD46 pKa = 3.21HH47 pKa = 7.68DD48 pKa = 4.0

Molecular weight: 5.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C6HCK7|A0A5C6HCK7_9BACT Uncharacterized protein OS=Bacteroidaceae bacterium HV4-6-C5C OX=2600309 GN=FQ707_14600 PE=4 SV=1
MM1 pKa = 7.58SVDD4 pKa = 3.76FCDD7 pKa = 3.52ARR9 pKa = 11.84QGGGAYY15 pKa = 10.08GKK17 pKa = 8.33TPATRR22 pKa = 11.84PFYY25 pKa = 10.82GSWPFAGLLLIRR37 pKa = 11.84HH38 pKa = 6.08PRR40 pKa = 11.84LYY42 pKa = 10.47PNDD45 pKa = 3.44RR46 pKa = 11.84AQRR49 pKa = 11.84VSEE52 pKa = 4.2RR53 pKa = 11.84GSGEE57 pKa = 3.62RR58 pKa = 11.84PIRR61 pKa = 11.84KK62 pKa = 6.18EE63 pKa = 3.66TAMTMLMQLARR74 pKa = 11.84QVSRR78 pKa = 11.84LEE80 pKa = 4.17SGQQ83 pKa = 3.37

Molecular weight: 9.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2881

0

2881

1119731

19

2890

388.7

43.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.761 ± 0.04

1.129 ± 0.014

5.521 ± 0.028

6.131 ± 0.043

4.59 ± 0.032

6.846 ± 0.04

1.858 ± 0.019

7.339 ± 0.044

6.933 ± 0.038

9.18 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.597 ± 0.02

5.511 ± 0.036

3.645 ± 0.023

3.365 ± 0.021

4.36 ± 0.031

6.628 ± 0.034

5.528 ± 0.031

6.215 ± 0.036

1.286 ± 0.021

4.576 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski