Arthrobacter phage BossLady

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Marthavirus; unclassified Marthavirus

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B8WME9|A0A5B8WME9_9CAUD Uncharacterized protein OS=Arthrobacter phage BossLady OX=2603258 GN=54 PE=4 SV=1
MM1 pKa = 7.1TAEE4 pKa = 4.09LCISNIHH11 pKa = 7.29DD12 pKa = 4.73DD13 pKa = 4.43DD14 pKa = 4.36PVEE17 pKa = 4.13GTVRR21 pKa = 11.84GTFEE25 pKa = 3.96TPHH28 pKa = 7.58AYY30 pKa = 9.79VVGVWYY36 pKa = 10.32CDD38 pKa = 3.08KK39 pKa = 11.11CSAAGQAFGMFTPDD53 pKa = 3.3DD54 pKa = 3.55TDD56 pKa = 3.48AAEE59 pKa = 4.63SEE61 pKa = 4.67IADD64 pKa = 3.32EE65 pKa = 4.34MTRR68 pKa = 11.84NDD70 pKa = 3.48HH71 pKa = 6.77KK72 pKa = 11.45

Molecular weight: 7.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B8WQ30|A0A5B8WQ30_9CAUD Uncharacterized protein OS=Arthrobacter phage BossLady OX=2603258 GN=60 PE=4 SV=1
MM1 pKa = 7.9IFIEE5 pKa = 4.14ARR7 pKa = 11.84RR8 pKa = 11.84SRR10 pKa = 11.84KK11 pKa = 9.52GRR13 pKa = 11.84WCDD16 pKa = 3.11QSFGHH21 pKa = 5.86QRR23 pKa = 11.84RR24 pKa = 11.84IEE26 pKa = 4.24PGDD29 pKa = 2.9WYY31 pKa = 11.0GRR33 pKa = 11.84VSVTPRR39 pKa = 11.84DD40 pKa = 3.99PYY42 pKa = 10.55MGAADD47 pKa = 3.18GRR49 pKa = 11.84MYY51 pKa = 10.87SFPVCLPCLLEE62 pKa = 4.36GVQGRR67 pKa = 11.84IAYY70 pKa = 8.72FSRR73 pKa = 11.84DD74 pKa = 3.1KK75 pKa = 10.97QGRR78 pKa = 11.84KK79 pKa = 9.36LVDD82 pKa = 4.83DD83 pKa = 3.83IVSRR87 pKa = 11.84RR88 pKa = 11.84AARR91 pKa = 11.84AA92 pKa = 3.08

Molecular weight: 10.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

79

0

79

16064

42

876

203.3

22.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.859 ± 0.453

0.716 ± 0.106

6.518 ± 0.3

6.25 ± 0.375

2.976 ± 0.148

7.414 ± 0.402

1.836 ± 0.165

5.024 ± 0.238

4.744 ± 0.23

7.893 ± 0.291

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.372 ± 0.153

3.461 ± 0.208

4.943 ± 0.267

3.816 ± 0.177

6.256 ± 0.375

5.64 ± 0.25

6.748 ± 0.335

7.719 ± 0.344

1.506 ± 0.128

2.31 ± 0.108
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski