Gemycircularvirus gemy-ch-rat1

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemycircularvirus; Gemycircularvirus ratas1; Rat associated gemycircularvirus 1

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M5IIA1|A0A0M5IIA1_9VIRU Uncharacterized protein OS=Gemycircularvirus gemy-ch-rat1 OX=1708653 PE=4 SV=1
MM1 pKa = 7.68PSSPMHH7 pKa = 5.94NVGDD11 pKa = 4.0LGFLGLLWGCSQRR24 pKa = 11.84IEE26 pKa = 4.03LSVPLEE32 pKa = 3.98EE33 pKa = 5.21SLTLMGEE40 pKa = 4.48FTSTLSSISADD51 pKa = 3.03SSAVEE56 pKa = 3.62RR57 pKa = 11.84LMFSMWEE64 pKa = 3.97AATLTYY70 pKa = 10.52RR71 pKa = 11.84SLEE74 pKa = 4.0GLQRR78 pKa = 11.84RR79 pKa = 11.84ATTTGSRR86 pKa = 11.84MAMWSQEE93 pKa = 4.02DD94 pKa = 3.85SNGRR98 pKa = 11.84YY99 pKa = 9.49LDD101 pKa = 3.67RR102 pKa = 11.84AEE104 pKa = 3.97VALARR109 pKa = 11.84LVLSGLQLRR118 pKa = 11.84WQRR121 pKa = 11.84TQEE124 pKa = 3.86NFGDD128 pKa = 5.28FVTSWLQNMSAALSTASGATQTGSSGLWMNPTLPPMEE165 pKa = 4.87YY166 pKa = 10.43GLTRR170 pKa = 11.84QRR172 pKa = 11.84LRR174 pKa = 11.84EE175 pKa = 4.14STSGSVRR182 pKa = 11.84LVWDD186 pKa = 4.34LDD188 pKa = 3.31HH189 pKa = 7.47WDD191 pKa = 3.3

Molecular weight: 21.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M5IIA1|A0A0M5IIA1_9VIRU Uncharacterized protein OS=Gemycircularvirus gemy-ch-rat1 OX=1708653 PE=4 SV=1
MM1 pKa = 7.2SRR3 pKa = 11.84KK4 pKa = 9.85KK5 pKa = 10.26ILNLTSIEE13 pKa = 4.12KK14 pKa = 10.38RR15 pKa = 11.84NTMLQYY21 pKa = 11.51ANTSTSGGSSVTIGPGPALINGGSNGFFILCPTAMDD57 pKa = 3.82LVEE60 pKa = 5.35RR61 pKa = 11.84GGANNSIVNAAEE73 pKa = 4.07RR74 pKa = 11.84NSTTRR79 pKa = 11.84YY80 pKa = 8.38MRR82 pKa = 11.84GFSEE86 pKa = 4.76NIRR89 pKa = 11.84IQTSTGLPWFWRR101 pKa = 11.84RR102 pKa = 11.84ICFCAKK108 pKa = 10.25NSIFNTFFSGDD119 pKa = 3.27TPTPNKK125 pKa = 9.95SSNVSYY131 pKa = 11.56VDD133 pKa = 3.15TTNGMEE139 pKa = 4.18RR140 pKa = 11.84LWFNQSVKK148 pKa = 9.09ITPLTQTAIQSVLFKK163 pKa = 10.89GISGQDD169 pKa = 2.9WTDD172 pKa = 3.18VLTAPVDD179 pKa = 3.43THH181 pKa = 7.61RR182 pKa = 11.84VDD184 pKa = 4.49LKK186 pKa = 10.51SDD188 pKa = 2.89KK189 pKa = 9.87MKK191 pKa = 10.8CIRR194 pKa = 11.84SGNASGAVSEE204 pKa = 4.24RR205 pKa = 11.84KK206 pKa = 9.04YY207 pKa = 9.98WYY209 pKa = 9.7PMNKK213 pKa = 9.54NLVLNDD219 pKa = 4.01DD220 pKa = 3.87EE221 pKa = 5.97SGEE224 pKa = 4.42KK225 pKa = 8.05MTTDD229 pKa = 3.63YY230 pKa = 11.47QSVLDD235 pKa = 3.85KK236 pKa = 11.51QEE238 pKa = 4.23DD239 pKa = 3.41GGYY242 pKa = 10.33YY243 pKa = 9.74IVDD246 pKa = 3.86VFQPGTGATSTDD258 pKa = 3.35QLQVV262 pKa = 3.05

Molecular weight: 28.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

775

191

322

258.3

28.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.968 ± 1.29

1.935 ± 0.669

5.032 ± 0.357

5.032 ± 0.75

3.871 ± 0.427

9.161 ± 0.776

1.935 ± 1.024

4.0 ± 1.069

3.613 ± 1.25

8.129 ± 2.03

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.097 ± 0.807

4.774 ± 1.339

3.742 ± 0.214

3.613 ± 0.627

6.71 ± 0.815

9.806 ± 1.485

6.968 ± 1.874

5.935 ± 0.631

2.968 ± 0.535

2.71 ± 0.386
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski