Lachnoclostridium sp. An14

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Lachnoclostridium; unclassified Lachnoclostridium

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3897 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y4RAF8|A0A1Y4RAF8_9FIRM DNA methyltransferase OS=Lachnoclostridium sp. An14 OX=1965562 GN=B5E84_16245 PE=3 SV=1
MM1 pKa = 7.31NMRR4 pKa = 11.84RR5 pKa = 11.84KK6 pKa = 9.85FFAAVSSLAVTSAVLLTGCGTPAQEE31 pKa = 3.95GTALKK36 pKa = 8.88EE37 pKa = 4.07TGILTLSVNPEE48 pKa = 3.34IQIEE52 pKa = 4.16YY53 pKa = 9.13NQDD56 pKa = 3.14GKK58 pKa = 9.82VTALTGRR65 pKa = 11.84NDD67 pKa = 3.58DD68 pKa = 3.47GKK70 pKa = 11.06GIVEE74 pKa = 4.83MYY76 pKa = 9.99PDD78 pKa = 4.5YY79 pKa = 10.81IGKK82 pKa = 8.87NCDD85 pKa = 3.24DD86 pKa = 3.94VLRR89 pKa = 11.84DD90 pKa = 4.23LIVEE94 pKa = 3.76IHH96 pKa = 5.92EE97 pKa = 4.67AGFFVDD103 pKa = 6.41DD104 pKa = 4.13IDD106 pKa = 4.27GNKK109 pKa = 10.06KK110 pKa = 10.61NIVLQLEE117 pKa = 4.52PGSVLPSNDD126 pKa = 2.94FLADD130 pKa = 3.64MSASTQEE137 pKa = 4.05AVKK140 pKa = 10.22EE141 pKa = 4.04LSLSSGIVTIDD152 pKa = 3.85DD153 pKa = 4.15DD154 pKa = 6.16DD155 pKa = 5.15YY156 pKa = 12.04DD157 pKa = 3.71SAYY160 pKa = 10.72AKK162 pKa = 10.35AGKK165 pKa = 8.81PSPYY169 pKa = 8.73ITLEE173 pKa = 3.83KK174 pKa = 10.31AQEE177 pKa = 3.66IALAQAHH184 pKa = 6.83ISAADD189 pKa = 3.34AVFDD193 pKa = 5.12DD194 pKa = 5.56KK195 pKa = 11.71EE196 pKa = 4.04FDD198 pKa = 4.03HH199 pKa = 7.97DD200 pKa = 4.44DD201 pKa = 3.55GTPIFEE207 pKa = 5.96LEE209 pKa = 4.17FTANGYY215 pKa = 10.28EE216 pKa = 4.03YY217 pKa = 10.11DD218 pKa = 3.96YY219 pKa = 10.75DD220 pKa = 3.4IHH222 pKa = 7.75AVTGKK227 pKa = 9.5VVKK230 pKa = 10.48AEE232 pKa = 4.19HH233 pKa = 6.54KK234 pKa = 10.92AGGAQNEE241 pKa = 4.63SLTTRR246 pKa = 11.84DD247 pKa = 3.45GSTDD251 pKa = 3.64YY252 pKa = 11.61NDD254 pKa = 3.51TDD256 pKa = 3.78YY257 pKa = 11.68GPNNDD262 pKa = 4.65GVTDD266 pKa = 4.02YY267 pKa = 11.63NDD269 pKa = 3.31TDD271 pKa = 3.76YY272 pKa = 11.68GPNNDD277 pKa = 4.65GVTDD281 pKa = 4.02YY282 pKa = 11.63NDD284 pKa = 3.31TDD286 pKa = 3.76YY287 pKa = 11.68GPNNDD292 pKa = 4.65GVTDD296 pKa = 4.02YY297 pKa = 11.79NDD299 pKa = 3.31TDD301 pKa = 4.04YY302 pKa = 12.06GDD304 pKa = 3.65TNYY307 pKa = 11.27DD308 pKa = 4.57DD309 pKa = 6.0GNTDD313 pKa = 3.96YY314 pKa = 11.81GDD316 pKa = 5.6DD317 pKa = 5.49DD318 pKa = 6.7DD319 pKa = 7.7DD320 pKa = 7.02DD321 pKa = 4.56DD322 pKa = 4.53

Molecular weight: 34.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y4R5F5|A0A1Y4R5F5_9FIRM Glutamine-dependent NAD(+) synthetase OS=Lachnoclostridium sp. An14 OX=1965562 GN=nadE PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.79KK9 pKa = 7.6RR10 pKa = 11.84QRR12 pKa = 11.84AKK14 pKa = 9.44VHH16 pKa = 5.57GFRR19 pKa = 11.84ARR21 pKa = 11.84MSSAGGRR28 pKa = 11.84KK29 pKa = 8.81VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.33GRR39 pKa = 11.84AKK41 pKa = 10.69LSAA44 pKa = 3.92

Molecular weight: 4.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3897

0

3897

1232716

34

3943

316.3

35.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.565 ± 0.045

1.479 ± 0.015

5.357 ± 0.035

7.829 ± 0.056

3.976 ± 0.028

8.097 ± 0.045

1.668 ± 0.018

6.164 ± 0.043

5.462 ± 0.037

9.324 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.078 ± 0.02

3.546 ± 0.023

3.847 ± 0.025

3.273 ± 0.02

5.483 ± 0.042

5.57 ± 0.035

5.337 ± 0.03

7.078 ± 0.031

1.056 ± 0.015

3.81 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski