Gordonia phage McGonagall

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 27 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A160DJQ6|A0A160DJQ6_9CAUD ABC transporter ATP-binding protein OS=Gordonia phage McGonagall OX=1838072 GN=27 PE=4 SV=1
MM1 pKa = 7.84PATTPAPYY9 pKa = 10.22SLPYY13 pKa = 10.58LVTSDD18 pKa = 4.68PLHH21 pKa = 6.69QIAQNTRR28 pKa = 11.84NLAEE32 pKa = 4.33RR33 pKa = 11.84LALLIASGEE42 pKa = 4.26LTGDD46 pKa = 3.58TGPANTLSIGTVTTAPAGDD65 pKa = 3.84PAAATISGIAPNQTLSLTLPRR86 pKa = 11.84GGQGDD91 pKa = 3.88TGPANTLSIGTVTTAPAGDD110 pKa = 3.84PAAATISGSAPNQTLSLTLPQGTGLHH136 pKa = 6.02LLGVVSTVAEE146 pKa = 4.51LGPAGSPGDD155 pKa = 3.77AKK157 pKa = 10.84LVLSDD162 pKa = 5.14PPHH165 pKa = 6.36LWVSDD170 pKa = 3.36GTSWHH175 pKa = 7.67DD176 pKa = 3.36SGQLQGPAGEE186 pKa = 4.36PAPDD190 pKa = 3.4QVTGAGLTLAVSSSPPAAGTPATTITVVSS219 pKa = 3.99

Molecular weight: 21.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A160DHC0|A0A160DHC0_9CAUD Uncharacterized protein OS=Gordonia phage McGonagall OX=1838072 GN=12 PE=4 SV=1
MM1 pKa = 6.81VATRR5 pKa = 11.84ARR7 pKa = 11.84FGGDD11 pKa = 3.12VVALAAPVRR20 pKa = 11.84SSDD23 pKa = 3.83DD24 pKa = 3.68LSDD27 pKa = 3.56VLMWSAAIAAFVSYY41 pKa = 10.16LGAGGRR47 pKa = 11.84SLEE50 pKa = 4.17TRR52 pKa = 11.84KK53 pKa = 9.75LRR55 pKa = 11.84QYY57 pKa = 11.27QLLCFADD64 pKa = 3.81VATAAPSAVEE74 pKa = 4.06LPEE77 pKa = 3.87LVAWLDD83 pKa = 3.53HH84 pKa = 6.51EE85 pKa = 4.76GWSNNTRR92 pKa = 11.84RR93 pKa = 11.84SVRR96 pKa = 11.84ACLVVFFTWLQATGQRR112 pKa = 11.84PDD114 pKa = 3.05NPARR118 pKa = 11.84LLPTVAAKK126 pKa = 10.14PGRR129 pKa = 11.84PRR131 pKa = 11.84PCPEE135 pKa = 3.5SALRR139 pKa = 11.84SAVVNAAPRR148 pKa = 11.84EE149 pKa = 3.97RR150 pKa = 11.84LMLALAATGGLRR162 pKa = 11.84RR163 pKa = 11.84AEE165 pKa = 3.78IAAVRR170 pKa = 11.84GEE172 pKa = 4.06HH173 pKa = 5.75VEE175 pKa = 4.03RR176 pKa = 11.84GFDD179 pKa = 3.64GAQLRR184 pKa = 11.84VLGKK188 pKa = 10.06GGRR191 pKa = 11.84EE192 pKa = 3.41RR193 pKa = 11.84LVPISDD199 pKa = 3.7EE200 pKa = 3.99LAEE203 pKa = 3.93QLEE206 pKa = 4.08QRR208 pKa = 11.84RR209 pKa = 11.84GYY211 pKa = 10.91AFPSPRR217 pKa = 11.84GGHH220 pKa = 5.94LTPAHH225 pKa = 5.78VGKK228 pKa = 10.72LIARR232 pKa = 11.84LLPPGWTTHH241 pKa = 4.72TLRR244 pKa = 11.84HH245 pKa = 5.89RR246 pKa = 11.84FASAAYY252 pKa = 9.79RR253 pKa = 11.84ADD255 pKa = 3.86RR256 pKa = 11.84DD257 pKa = 3.24IRR259 pKa = 11.84AVQEE263 pKa = 4.27LLGHH267 pKa = 6.66ASVATTQIYY276 pKa = 7.54TALPDD281 pKa = 3.89DD282 pKa = 4.01SLRR285 pKa = 11.84RR286 pKa = 11.84ATLSATTFAAVAA298 pKa = 3.8

Molecular weight: 32.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

27

0

27

5413

33

657

200.5

21.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.576 ± 0.806

0.887 ± 0.202

6.263 ± 0.649

3.769 ± 0.439

2.476 ± 0.203

8.406 ± 0.72

2.161 ± 0.301

4.194 ± 0.607

2.346 ± 0.256

8.72 ± 0.426

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.014 ± 0.175

2.512 ± 0.285

6.484 ± 0.474

2.937 ± 0.232

6.392 ± 0.713

6.281 ± 0.374

7.741 ± 0.448

8.239 ± 0.6

1.774 ± 0.121

1.829 ± 0.258
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski