Pseudidiomarina aestuarii

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Idiomarinaceae; Pseudidiomarina

Average proteome isoelectric point is 5.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 947 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T4D981|A0A2T4D981_9GAMM Cellulose synthase catalytic subunit [UDP-forming] OS=Pseudidiomarina aestuarii OX=624146 GN=bcsA PE=3 SV=1
MM1 pKa = 7.12TAGLRR6 pKa = 11.84YY7 pKa = 9.34LAILSLAFTLAACGSGEE24 pKa = 4.21RR25 pKa = 11.84NTGGGDD31 pKa = 3.08TGGDD35 pKa = 3.45GGGSGSTPPTATASNVTVNEE55 pKa = 4.1GNPNQVIEE63 pKa = 4.09AAITVSLSTTANASVSFDD81 pKa = 3.61YY82 pKa = 9.48ATADD86 pKa = 3.23ASGLAGTDD94 pKa = 3.64YY95 pKa = 11.1EE96 pKa = 4.52DD97 pKa = 3.5TSGTVTFTAGQSSQTITIPIISNAVFNDD125 pKa = 3.11DD126 pKa = 3.68RR127 pKa = 11.84DD128 pKa = 4.04FFVDD132 pKa = 4.54FSNAQGLTLGTTRR145 pKa = 11.84VQVTIVNDD153 pKa = 3.59DD154 pKa = 3.77PRR156 pKa = 11.84PVLAFEE162 pKa = 3.9QSIFEE167 pKa = 4.35TTEE170 pKa = 3.56QAGTLGLTVTLSNGTEE186 pKa = 4.08SEE188 pKa = 4.46VTFDD192 pKa = 4.62LDD194 pKa = 3.8VSGTATAGVDD204 pKa = 3.51YY205 pKa = 10.77SLEE208 pKa = 4.25EE209 pKa = 3.87TSFTIPAGALEE220 pKa = 4.09QQISLTLIADD230 pKa = 3.8DD231 pKa = 3.82VKK233 pKa = 11.23EE234 pKa = 4.36GGEE237 pKa = 4.31SVILAFANVRR247 pKa = 11.84EE248 pKa = 4.3ADD250 pKa = 3.94TNPDD254 pKa = 3.57LSTQTIVIRR263 pKa = 11.84GDD265 pKa = 3.79AILADD270 pKa = 3.92TGAVEE275 pKa = 4.8YY276 pKa = 10.81YY277 pKa = 10.82NSGDD281 pKa = 3.58FSAATPDD288 pKa = 3.96AEE290 pKa = 5.54HH291 pKa = 7.35PNQDD295 pKa = 2.74ADD297 pKa = 3.76YY298 pKa = 11.23GRR300 pKa = 11.84DD301 pKa = 2.94TTAAVDD307 pKa = 3.81NNADD311 pKa = 3.43GAGNLNLTKK320 pKa = 10.3LDD322 pKa = 3.54SSGNALPQNATSFSCVRR339 pKa = 11.84DD340 pKa = 3.78NLSGTVYY347 pKa = 10.75AVWAEE352 pKa = 3.8GDD354 pKa = 3.67ALSNFYY360 pKa = 10.91FNPTYY365 pKa = 10.21RR366 pKa = 11.84YY367 pKa = 9.81KK368 pKa = 10.55WFNDD372 pKa = 3.11DD373 pKa = 3.44STNNAGNPGTFTPEE387 pKa = 3.62SAFPAFPDD395 pKa = 3.62EE396 pKa = 4.51NDD398 pKa = 3.94PIYY401 pKa = 10.94YY402 pKa = 10.4EE403 pKa = 4.14NANCAFPAVDD413 pKa = 4.32PVVQTLGCSSQNYY426 pKa = 7.87TNYY429 pKa = 10.53LNTIGYY435 pKa = 9.79CGFDD439 pKa = 3.87DD440 pKa = 3.63WRR442 pKa = 11.84LPTISEE448 pKa = 4.17LQSIAIYY455 pKa = 10.27QADD458 pKa = 3.62QSQDD462 pKa = 3.05NLFFPDD468 pKa = 3.47VAMMTPNGDD477 pKa = 3.21GTVRR481 pKa = 11.84LLSSTPAADD490 pKa = 3.98PNSSAWCLEE499 pKa = 3.89VQTARR504 pKa = 11.84RR505 pKa = 11.84MLCNKK510 pKa = 9.85NDD512 pKa = 3.61VNSIRR517 pKa = 11.84AARR520 pKa = 11.84NPVAYY525 pKa = 10.27QEE527 pKa = 4.18

Molecular weight: 55.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T4CVT6|A0A2T4CVT6_9GAMM Thioesterase family protein OS=Pseudidiomarina aestuarii OX=624146 GN=C9927_02105 PE=4 SV=1
MM1 pKa = 7.42KK2 pKa = 10.05RR3 pKa = 11.84QKK5 pKa = 10.3RR6 pKa = 11.84DD7 pKa = 3.2RR8 pKa = 11.84LEE10 pKa = 3.91RR11 pKa = 11.84AHH13 pKa = 6.72AQGFKK18 pKa = 10.51AGVSGRR24 pKa = 11.84SKK26 pKa = 10.43EE27 pKa = 3.87LCPYY31 pKa = 9.75QNQDD35 pKa = 5.21ARR37 pKa = 11.84SQWLGGWRR45 pKa = 11.84DD46 pKa = 3.18AMEE49 pKa = 4.08ARR51 pKa = 11.84GVGLYY56 pKa = 9.9RR57 pKa = 5.07

Molecular weight: 6.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

947

0

947

274386

33

1619

289.7

32.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.898 ± 0.084

0.836 ± 0.026

5.657 ± 0.063

6.399 ± 0.083

3.997 ± 0.061

6.988 ± 0.085

2.146 ± 0.042

5.801 ± 0.073

3.868 ± 0.069

10.611 ± 0.081

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.394 ± 0.039

3.501 ± 0.051

4.248 ± 0.048

5.044 ± 0.078

5.678 ± 0.07

6.013 ± 0.064

5.374 ± 0.056

7.261 ± 0.075

1.36 ± 0.035

2.924 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski