Macaca nemestrina rhadinovirus 2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes; Herpesvirales; Herpesviridae; Gammaherpesvirinae; Rhadinovirus; Macacine gammaherpesvirus 12

Average proteome isoelectric point is 7.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 95 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0B5CYF8|A0A0B5CYF8_9GAMA ORF49 OS=Macaca nemestrina rhadinovirus 2 OX=123630 GN=ORF49 PE=4 SV=1
MM1 pKa = 7.9AMFLSDD7 pKa = 4.33PPRR10 pKa = 11.84TPPATPRR17 pKa = 11.84MLPIPGAPKK26 pKa = 9.82KK27 pKa = 9.09RR28 pKa = 11.84TRR30 pKa = 11.84RR31 pKa = 11.84FLFAGAGTALPVPPGYY47 pKa = 10.1QGPRR51 pKa = 11.84VIDD54 pKa = 3.3MSAPDD59 pKa = 3.91SVFDD63 pKa = 4.91LDD65 pKa = 5.44SPPTTPKK72 pKa = 10.49TPDD75 pKa = 3.2EE76 pKa = 4.19TDD78 pKa = 3.13SRR80 pKa = 11.84SEE82 pKa = 3.87DD83 pKa = 3.52SNYY86 pKa = 10.75DD87 pKa = 3.43DD88 pKa = 5.41SDD90 pKa = 3.75VDD92 pKa = 3.74EE93 pKa = 4.79EE94 pKa = 5.09TNTEE98 pKa = 4.24PPVSSPPKK106 pKa = 9.67IGLALSEE113 pKa = 4.37GDD115 pKa = 4.21LFRR118 pKa = 11.84PSNTRR123 pKa = 11.84PSVIVEE129 pKa = 4.35SGPVAQPNAPAPLTAFGGPRR149 pKa = 11.84PVAVVTGQHH158 pKa = 6.35RR159 pKa = 11.84APPSSDD165 pKa = 3.45SNSDD169 pKa = 3.36DD170 pKa = 4.48FFIDD174 pKa = 4.09DD175 pKa = 4.5YY176 pKa = 11.62EE177 pKa = 6.35DD178 pKa = 3.92NDD180 pKa = 3.91EE181 pKa = 4.95SDD183 pKa = 4.91NDD185 pKa = 4.14DD186 pKa = 3.71EE187 pKa = 4.92TDD189 pKa = 3.36GFSPRR194 pKa = 11.84GSTGPWPGDD203 pKa = 3.44VSRR206 pKa = 11.84SPPEE210 pKa = 4.42GDD212 pKa = 2.92WSSDD216 pKa = 3.41DD217 pKa = 4.93DD218 pKa = 3.96LTEE221 pKa = 3.98EE222 pKa = 4.03TGAAAGEE229 pKa = 4.03EE230 pKa = 4.31TIIISSSDD238 pKa = 3.96DD239 pKa = 3.61EE240 pKa = 6.71DD241 pKa = 5.37EE242 pKa = 5.68DD243 pKa = 4.17DD244 pKa = 3.92QNSVGTEE251 pKa = 3.91DD252 pKa = 4.96EE253 pKa = 4.13FDD255 pKa = 3.89ANAGSGGADD264 pKa = 3.46VIDD267 pKa = 4.57LCSSSDD273 pKa = 3.5SDD275 pKa = 5.13DD276 pKa = 3.96EE277 pKa = 4.41VDD279 pKa = 3.75GAVGGARR286 pKa = 11.84ANCKK290 pKa = 9.71RR291 pKa = 11.84RR292 pKa = 11.84ASRR295 pKa = 11.84LDD297 pKa = 3.98DD298 pKa = 4.85DD299 pKa = 5.05SEE301 pKa = 5.72DD302 pKa = 4.32DD303 pKa = 3.75IIYY306 pKa = 10.83VGTTQGRR313 pKa = 11.84KK314 pKa = 9.15RR315 pKa = 11.84RR316 pKa = 11.84VTSTAKK322 pKa = 10.63GGATSSSEE330 pKa = 3.94GAGVSGRR337 pKa = 11.84QNMAATPPVCGNDD350 pKa = 3.26NYY352 pKa = 10.38PWPWLDD358 pKa = 3.15

Molecular weight:
37.69 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A0B5D3J3|A0A0B5D3J3_9GAMA Major capsid protein OS=Macaca nemestrina rhadinovirus 2 OX=123630 GN=ORF25 PE=3 SV=1
MM1 pKa = 7.48SLDD4 pKa = 3.4NQHH7 pKa = 6.07RR8 pKa = 11.84HH9 pKa = 4.71SAALEE14 pKa = 4.0CLPTARR20 pKa = 11.84KK21 pKa = 9.03RR22 pKa = 11.84AGIRR26 pKa = 11.84AHH28 pKa = 5.85LAVYY32 pKa = 10.09RR33 pKa = 11.84RR34 pKa = 11.84LIKK37 pKa = 10.3HH38 pKa = 6.52RR39 pKa = 11.84SLGDD43 pKa = 2.99IFKK46 pKa = 10.49FLSICSTRR54 pKa = 11.84EE55 pKa = 3.79ATEE58 pKa = 4.05DD59 pKa = 3.15AQFRR63 pKa = 11.84IFFEE67 pKa = 4.12VTLGKK72 pKa = 10.47RR73 pKa = 11.84IADD76 pKa = 3.44CVLTVEE82 pKa = 4.97SKK84 pKa = 10.16NQKK87 pKa = 6.26TCYY90 pKa = 9.65VVEE93 pKa = 4.86LKK95 pKa = 9.6TCLSGFVFPGSAIKK109 pKa = 10.43ISQRR113 pKa = 11.84RR114 pKa = 11.84QGLEE118 pKa = 3.57QLTDD122 pKa = 3.31SVAYY126 pKa = 9.51IGRR129 pKa = 11.84AAPKK133 pKa = 10.09GHH135 pKa = 6.51EE136 pKa = 4.15NWSVRR141 pKa = 11.84PLLLFKK147 pKa = 10.55NQKK150 pKa = 5.53TLKK153 pKa = 8.91TIHH156 pKa = 6.4TEE158 pKa = 3.89SSAFPPTFINTTSVALNGFFNQWEE182 pKa = 4.24DD183 pKa = 3.25ARR185 pKa = 11.84VRR187 pKa = 11.84KK188 pKa = 8.62MLYY191 pKa = 9.97AIPAKK196 pKa = 9.58THH198 pKa = 5.32ATNNLEE204 pKa = 4.21FLDD207 pKa = 4.34PASKK211 pKa = 10.09QRR213 pKa = 11.84SVYY216 pKa = 10.4SQTIVNGGKK225 pKa = 9.91KK226 pKa = 9.79KK227 pKa = 10.08RR228 pKa = 11.84VRR230 pKa = 11.84DD231 pKa = 3.75AKK233 pKa = 10.05STAGAQGSRR242 pKa = 11.84AEE244 pKa = 4.74KK245 pKa = 10.38KK246 pKa = 9.19PAPARR251 pKa = 11.84ARR253 pKa = 11.84QRR255 pKa = 11.84AANAPTGNGDD265 pKa = 3.35GYY267 pKa = 11.32ARR269 pKa = 11.84HH270 pKa = 6.62RR271 pKa = 11.84DD272 pKa = 3.69DD273 pKa = 4.01PRR275 pKa = 11.84HH276 pKa = 5.03GRR278 pKa = 11.84RR279 pKa = 11.84FASTEE284 pKa = 3.51QKK286 pKa = 9.74PRR288 pKa = 11.84RR289 pKa = 11.84NRR291 pKa = 11.84TVNRR295 pKa = 11.84PATQNRR301 pKa = 11.84PSDD304 pKa = 2.92AWRR307 pKa = 11.84HH308 pKa = 4.11VRR310 pKa = 11.84GHH312 pKa = 5.96NSPRR316 pKa = 11.84GRR318 pKa = 11.84GFYY321 pKa = 10.28GKK323 pKa = 9.36PGSPSGAPARR333 pKa = 11.84SVHH336 pKa = 5.66EE337 pKa = 4.44PKK339 pKa = 10.69PMAATVRR346 pKa = 11.84SVVQQ350 pKa = 3.29

Molecular weight:
39.06 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

85

10

95

41158

70

2554

433.2

48.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.889 ± 0.237

2.488 ± 0.151

4.84 ± 0.153

5.005 ± 0.133

4.39 ± 0.148

5.919 ± 0.194

2.707 ± 0.094

4.816 ± 0.162

4.087 ± 0.197

10.183 ± 0.249

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.065 ± 0.106

4.019 ± 0.156

6.434 ± 0.284

3.705 ± 0.152

5.979 ± 0.194

7.389 ± 0.181

6.521 ± 0.252

7.199 ± 0.198

1.2 ± 0.083

3.166 ± 0.12

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski