Firmicutes bacterium CAG:114

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; environmental samples

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2222 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5HG04|R5HG04_9FIRM Uncharacterized protein OS=Firmicutes bacterium CAG:114 OX=1263001 GN=BN469_01834 PE=4 SV=1
MM1 pKa = 7.62KK2 pKa = 10.56KK3 pKa = 9.21MLALTLALVMALGLCACGGDD23 pKa = 3.62TTDD26 pKa = 5.03NGDD29 pKa = 3.79APAEE33 pKa = 4.05NPEE36 pKa = 4.37TNSPAEE42 pKa = 4.28TVTLDD47 pKa = 3.72LPPVEE52 pKa = 5.6DD53 pKa = 3.76LTGTDD58 pKa = 3.23TSAIAGVEE66 pKa = 4.04DD67 pKa = 4.2GVLTVAMEE75 pKa = 4.34CTYY78 pKa = 11.43APYY81 pKa = 10.94NWTQMDD87 pKa = 4.23DD88 pKa = 3.66SNGAVPIKK96 pKa = 10.62GSSEE100 pKa = 3.94YY101 pKa = 11.38ANGYY105 pKa = 10.54DD106 pKa = 3.6VMIAKK111 pKa = 9.28RR112 pKa = 11.84ICEE115 pKa = 3.9AYY117 pKa = 9.46GWEE120 pKa = 4.09LEE122 pKa = 4.72IVRR125 pKa = 11.84TDD127 pKa = 3.11WNSLVPGIQSGLYY140 pKa = 9.25DD141 pKa = 3.87AVIAGQSMTEE151 pKa = 3.81DD152 pKa = 2.78RR153 pKa = 11.84MEE155 pKa = 4.11QVDD158 pKa = 4.07FAGPYY163 pKa = 8.1YY164 pKa = 9.88YY165 pKa = 11.17ASIVCVAKK173 pKa = 10.74DD174 pKa = 3.61GSPQASAKK182 pKa = 10.37GISEE186 pKa = 4.3LTGTATAQIEE196 pKa = 4.8TIWYY200 pKa = 6.97DD201 pKa = 3.21TCLPQIKK208 pKa = 9.81GAEE211 pKa = 4.0IQTAAEE217 pKa = 4.03TAPAMLMALEE227 pKa = 4.36TGAVEE232 pKa = 6.23FICTDD237 pKa = 2.7IPTAKK242 pKa = 10.1GAVAAYY248 pKa = 10.28DD249 pKa = 3.74DD250 pKa = 4.25MVILDD255 pKa = 3.94FTDD258 pKa = 3.53TEE260 pKa = 5.23DD261 pKa = 3.61NFQVDD266 pKa = 3.8EE267 pKa = 4.3GDD269 pKa = 3.48INIGISLKK277 pKa = 10.69KK278 pKa = 10.82GNTEE282 pKa = 4.59LKK284 pKa = 10.51DD285 pKa = 4.17AINKK289 pKa = 8.14VVSALNKK296 pKa = 10.77DD297 pKa = 3.6NFDD300 pKa = 3.7NLMDD304 pKa = 4.04YY305 pKa = 10.71AVSIQPLNQQ314 pKa = 3.08

Molecular weight: 33.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5HBE7|R5HBE7_9FIRM Predicted transcriptional regulator OS=Firmicutes bacterium CAG:114 OX=1263001 GN=BN469_01744 PE=4 SV=1
MM1 pKa = 7.06VRR3 pKa = 11.84TYY5 pKa = 10.22QPKK8 pKa = 9.24KK9 pKa = 7.68RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.69EE15 pKa = 3.5HH16 pKa = 6.14GFRR19 pKa = 11.84KK20 pKa = 9.99RR21 pKa = 11.84MSTANGRR28 pKa = 11.84KK29 pKa = 8.58VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84ARR41 pKa = 11.84LTHH44 pKa = 6.31

Molecular weight: 5.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2222

0

2222

642334

29

1845

289.1

31.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.542 ± 0.056

1.562 ± 0.024

5.178 ± 0.048

6.492 ± 0.054

3.744 ± 0.043

8.283 ± 0.067

1.983 ± 0.032

5.264 ± 0.049

4.395 ± 0.049

10.62 ± 0.083

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.616 ± 0.028

3.037 ± 0.036

4.499 ± 0.039

4.209 ± 0.039

5.726 ± 0.066

5.333 ± 0.042

5.805 ± 0.06

7.32 ± 0.043

1.102 ± 0.022

3.286 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski