Alteromonas australica

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Alteromonadaceae; Alteromonas

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3445 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A075P430|A0A075P430_9ALTE Ligand-gated channel OS=Alteromonas australica OX=589873 GN=EP13_18085 PE=3 SV=1
MM1 pKa = 7.68RR2 pKa = 11.84KK3 pKa = 9.64ALTLLTALFTSVSATLSFPALADD26 pKa = 3.81TPDD29 pKa = 2.72WRR31 pKa = 11.84YY32 pKa = 10.58VEE34 pKa = 4.61GGYY37 pKa = 8.73TKK39 pKa = 9.95IDD41 pKa = 3.65FDD43 pKa = 5.17DD44 pKa = 5.3DD45 pKa = 4.02EE46 pKa = 5.59SFEE49 pKa = 4.45PDD51 pKa = 3.12GFNVSSRR58 pKa = 11.84YY59 pKa = 9.83LLNSNIFLNGEE70 pKa = 4.0YY71 pKa = 10.58NRR73 pKa = 11.84VEE75 pKa = 4.27EE76 pKa = 4.92GSFDD80 pKa = 4.78LDD82 pKa = 5.54LITLGAGYY90 pKa = 10.55RR91 pKa = 11.84MPINSTTDD99 pKa = 2.72AYY101 pKa = 9.83FTANFEE107 pKa = 4.35QLDD110 pKa = 3.71ADD112 pKa = 3.75ATDD115 pKa = 3.57EE116 pKa = 4.12NGYY119 pKa = 10.15SINAGLRR126 pKa = 11.84SMVTEE131 pKa = 3.93QIEE134 pKa = 4.35LNGEE138 pKa = 3.36IGYY141 pKa = 10.59YY142 pKa = 10.51DD143 pKa = 3.79VFDD146 pKa = 4.56GEE148 pKa = 4.39ATIKK152 pKa = 10.36IGANYY157 pKa = 9.97YY158 pKa = 7.46VTPQWAFGASYY169 pKa = 10.93KK170 pKa = 10.91SVDD173 pKa = 4.51DD174 pKa = 5.15LDD176 pKa = 3.8ILQVNARR183 pKa = 11.84YY184 pKa = 9.93AFF186 pKa = 3.86

Molecular weight: 20.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A075P3P4|A0A075P3P4_9ALTE DNA-directed RNA polymerase subunit beta OS=Alteromonas australica OX=589873 GN=rpoB PE=3 SV=1
MM1 pKa = 8.1ADD3 pKa = 3.04TQYY6 pKa = 11.37YY7 pKa = 7.4GTGRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 10.28SSTARR18 pKa = 11.84VFLRR22 pKa = 11.84PGTGSIKK29 pKa = 10.13VNQRR33 pKa = 11.84ALDD36 pKa = 3.8EE37 pKa = 4.39YY38 pKa = 10.72FGRR41 pKa = 11.84EE42 pKa = 4.14TEE44 pKa = 4.32CMVVRR49 pKa = 11.84QPLEE53 pKa = 3.89LVEE56 pKa = 4.12MTEE59 pKa = 5.13KK60 pKa = 10.44FDD62 pKa = 5.49LYY64 pKa = 10.12ITVKK68 pKa = 10.81GGGSNGQAGAIRR80 pKa = 11.84HH81 pKa = 6.31GITRR85 pKa = 11.84ALMEE89 pKa = 4.35YY90 pKa = 10.8DD91 pKa = 3.3EE92 pKa = 5.0ALRR95 pKa = 11.84PALRR99 pKa = 11.84KK100 pKa = 10.04AGFVTRR106 pKa = 11.84DD107 pKa = 2.94ARR109 pKa = 11.84RR110 pKa = 11.84VEE112 pKa = 4.08RR113 pKa = 11.84KK114 pKa = 9.57KK115 pKa = 11.18VGLHH119 pKa = 6.16KK120 pKa = 10.55ARR122 pKa = 11.84KK123 pKa = 8.85RR124 pKa = 11.84PQFSKK129 pKa = 10.9RR130 pKa = 3.29

Molecular weight: 14.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3445

0

3445

1183837

37

5173

343.6

38.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.085 ± 0.048

0.961 ± 0.014

5.856 ± 0.037

6.165 ± 0.036

4.214 ± 0.026

6.826 ± 0.04

2.306 ± 0.024

6.033 ± 0.029

5.043 ± 0.043

9.991 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.501 ± 0.022

4.369 ± 0.027

3.954 ± 0.023

4.237 ± 0.029

4.456 ± 0.032

6.745 ± 0.032

5.733 ± 0.051

7.188 ± 0.033

1.197 ± 0.016

3.141 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski