Abalone shriveling syndrome-associated virus

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 31 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B8Q5C9|B8Q5C9_9CAUD Uncharacterized protein OS=Abalone shriveling syndrome-associated virus OX=491893 PE=4 SV=1
MM1 pKa = 8.38VEE3 pKa = 4.15FSKK6 pKa = 10.72IKK8 pKa = 10.46SDD10 pKa = 3.79LGSVAYY16 pKa = 9.79IEE18 pKa = 5.95DD19 pKa = 3.77SADD22 pKa = 3.56LNKK25 pKa = 9.89VPLSAVDD32 pKa = 3.38AGGFFYY38 pKa = 10.35WKK40 pKa = 9.71AANNMTPEE48 pKa = 4.66LSSADD53 pKa = 2.94IGKK56 pKa = 10.3AYY58 pKa = 10.45GITTVDD64 pKa = 2.88ASGAVTFGGVDD75 pKa = 3.42AFLLDD80 pKa = 4.42DD81 pKa = 4.08ATASIINQAGIIEE94 pKa = 4.94PPTLSITGTNTINVGPFKK112 pKa = 10.77AVFFDD117 pKa = 3.88QPAGAVGNVRR127 pKa = 11.84RR128 pKa = 11.84VVDD131 pKa = 3.89SPVQQTITINAVGSSNQDD149 pKa = 2.77VTYY152 pKa = 11.02LKK154 pKa = 10.16FLPSGSFAQEE164 pKa = 3.84TDD166 pKa = 3.48DD167 pKa = 3.87PTADD171 pKa = 5.26RR172 pKa = 11.84IIEE175 pKa = 4.11DD176 pKa = 3.61NYY178 pKa = 10.13IVRR181 pKa = 11.84ILHH184 pKa = 6.04PNGGKK189 pKa = 9.39IVGLSPVSTVYY200 pKa = 11.1SNPQQNLQQYY210 pKa = 8.18SSRR213 pKa = 11.84IGIDD217 pKa = 3.2GNDD220 pKa = 3.11ILFSISNNSLRR231 pKa = 11.84QTGANARR238 pKa = 11.84IFGYY242 pKa = 10.66NIADD246 pKa = 3.63GSGTGNRR253 pKa = 11.84SLRR256 pKa = 11.84TIAVEE261 pKa = 5.76DD262 pKa = 3.39ITADD266 pKa = 3.63YY267 pKa = 11.27YY268 pKa = 11.8SFDD271 pKa = 3.66STEE274 pKa = 3.65QTTFSDD280 pKa = 3.96LRR282 pKa = 11.84RR283 pKa = 11.84TYY285 pKa = 11.23KK286 pKa = 10.61LDD288 pKa = 3.86DD289 pKa = 4.18PAAGTSSALANFGIIAVYY307 pKa = 10.1ILASGSYY314 pKa = 10.64YY315 pKa = 10.93YY316 pKa = 10.87LAPQKK321 pKa = 10.65NYY323 pKa = 9.11STAALARR330 pKa = 11.84SDD332 pKa = 3.81LLFYY336 pKa = 10.48LTGTVVPDD344 pKa = 4.1FLSSSFAALLGAIVVPATVTDD365 pKa = 4.33ANISTVLTTTISNISLASSGGGVQTLTIDD394 pKa = 3.85PSTGTNDD401 pKa = 4.26DD402 pKa = 3.73VVGTNGTNFILRR414 pKa = 11.84KK415 pKa = 9.41EE416 pKa = 4.04NAFNVTGGNEE426 pKa = 3.66GDD428 pKa = 3.78VLVKK432 pKa = 10.48RR433 pKa = 11.84GTSFAVEE440 pKa = 4.7PIANQGVPRR449 pKa = 11.84FNLGTDD455 pKa = 2.32RR456 pKa = 11.84GGFVKK461 pKa = 10.73LDD463 pKa = 3.54VSNGAIVSSNGDD475 pKa = 3.16IASDD479 pKa = 4.13FSSAIIRR486 pKa = 11.84QVNDD490 pKa = 3.12AYY492 pKa = 10.89YY493 pKa = 10.03ISLISISTTKK503 pKa = 10.1IPVWISSYY511 pKa = 11.25VLNTDD516 pKa = 3.22GNMMPVTPTADD527 pKa = 2.9ISIDD531 pKa = 3.87FIVPVVLTPAQPEE544 pKa = 3.88FLLRR548 pKa = 11.84YY549 pKa = 9.62ILVNKK554 pKa = 9.95SS555 pKa = 2.69

Molecular weight: 58.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B8Q5C2|B8Q5C2_9CAUD Uncharacterized protein OS=Abalone shriveling syndrome-associated virus OX=491893 PE=4 SV=1
MM1 pKa = 7.58ALFAFAALASIAAAGFGACRR21 pKa = 11.84NSRR24 pKa = 11.84EE25 pKa = 3.67NSRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 9.64VLLRR34 pKa = 11.84NEE36 pKa = 4.13LVAQEE41 pKa = 4.13EE42 pKa = 4.35LLNRR46 pKa = 11.84EE47 pKa = 3.75RR48 pKa = 11.84RR49 pKa = 11.84LRR51 pKa = 11.84AAGINAFQRR60 pKa = 11.84RR61 pKa = 11.84GSTAFDD67 pKa = 4.2DD68 pKa = 5.2LDD70 pKa = 5.55DD71 pKa = 4.41EE72 pKa = 5.04FSLRR76 pKa = 11.84GNSLNANTYY85 pKa = 7.51SRR87 pKa = 11.84RR88 pKa = 11.84VANPGLTDD96 pKa = 3.58SFADD100 pKa = 4.12LSRR103 pKa = 11.84GVVKK107 pKa = 10.46AAEE110 pKa = 3.88FLINRR115 pKa = 11.84SKK117 pKa = 11.27LGGG120 pKa = 3.47

Molecular weight: 13.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

31

0

31

10892

115

1194

351.4

38.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.456 ± 0.547

0.826 ± 0.21

5.812 ± 0.356

4.912 ± 0.349

5.224 ± 0.252

6.023 ± 0.43

1.451 ± 0.243

6.721 ± 0.285

5.922 ± 0.418

9.236 ± 0.341

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.836 ± 0.168

5.913 ± 0.303

3.819 ± 0.248

3.452 ± 0.183

4.361 ± 0.308

8.336 ± 0.347

6.941 ± 0.365

6.62 ± 0.346

0.633 ± 0.089

3.507 ± 0.258
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski