San Bernardo virus

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1U7EIV6|A0A1U7EIV6_9VIRU Non-structural protein 3 OS=San Bernardo virus OX=1955199 PE=4 SV=1
MM1 pKa = 7.49KK2 pKa = 10.36FLVLFVSVVNAAVFVKK18 pKa = 10.62DD19 pKa = 3.4VTRR22 pKa = 11.84QLATTRR28 pKa = 11.84EE29 pKa = 4.05RR30 pKa = 11.84YY31 pKa = 9.48ALYY34 pKa = 10.67QNLSPTLVKK43 pKa = 10.24IGNYY47 pKa = 9.22QISPFHH53 pKa = 7.35PDD55 pKa = 3.35PACVSVYY62 pKa = 8.95RR63 pKa = 11.84TNDD66 pKa = 2.48WFFAGCEE73 pKa = 4.21LPSHH77 pKa = 6.53CLGITVSVVEE87 pKa = 4.49KK88 pKa = 10.14KK89 pKa = 9.31WYY91 pKa = 8.56GQEE94 pKa = 4.06TVFCHH99 pKa = 5.5ATYY102 pKa = 10.53HH103 pKa = 6.76PDD105 pKa = 3.12RR106 pKa = 11.84TEE108 pKa = 3.54TYY110 pKa = 8.73EE111 pKa = 3.96FYY113 pKa = 10.86NIEE116 pKa = 4.03FRR118 pKa = 11.84PSEE121 pKa = 3.9RR122 pKa = 11.84KK123 pKa = 9.37VRR125 pKa = 11.84PKK127 pKa = 10.93SPLKK131 pKa = 10.81LYY133 pKa = 10.68GKK135 pKa = 10.53VITEE139 pKa = 4.11ISNLYY144 pKa = 8.62PVSFFEE150 pKa = 4.42YY151 pKa = 9.84FVVARR156 pKa = 11.84NEE158 pKa = 4.29SGFGLVPKK166 pKa = 10.46FCMEE170 pKa = 4.0KK171 pKa = 10.49LRR173 pKa = 11.84NGTQLPKK180 pKa = 10.5NVQLRR185 pKa = 11.84HH186 pKa = 5.5SEE188 pKa = 3.88GMVCVDD194 pKa = 3.01EE195 pKa = 4.71VNYY198 pKa = 10.51DD199 pKa = 3.3EE200 pKa = 5.74HH201 pKa = 7.47DD202 pKa = 3.49CHH204 pKa = 7.75PYY206 pKa = 9.35MFSTVMEE213 pKa = 4.55MIMFYY218 pKa = 10.73DD219 pKa = 4.4FPIEE223 pKa = 4.0FTDD226 pKa = 3.72VPFSQGSFAATNNKK240 pKa = 9.73AMGADD245 pKa = 3.21AYY247 pKa = 10.15VANHH251 pKa = 6.19ASEE254 pKa = 4.69VPSHH258 pKa = 6.33VFDD261 pKa = 4.87KK262 pKa = 10.55IGRR265 pKa = 11.84SFMVSFVKK273 pKa = 8.27MTSSDD278 pKa = 3.17PFFIVKK284 pKa = 8.01NTRR287 pKa = 11.84KK288 pKa = 10.27LEE290 pKa = 4.18VIQTHH295 pKa = 6.8CYY297 pKa = 10.41DD298 pKa = 3.59FHH300 pKa = 6.18THH302 pKa = 5.4YY303 pKa = 10.73KK304 pKa = 10.46SPFSNFLSTVSKK316 pKa = 10.1FIQDD320 pKa = 3.55EE321 pKa = 4.02LSEE324 pKa = 4.18FLRR327 pKa = 11.84FLKK330 pKa = 10.61KK331 pKa = 10.18FSKK334 pKa = 10.57SIAEE338 pKa = 3.69ILLFVVSEE346 pKa = 4.25LLLMVADD353 pKa = 5.42LIPYY357 pKa = 8.67TGEE360 pKa = 3.97FYY362 pKa = 10.45TGAFITLVFYY372 pKa = 9.15YY373 pKa = 7.78TTFNIGVSAVAGLLVYY389 pKa = 8.17FFRR392 pKa = 11.84IYY394 pKa = 10.19IDD396 pKa = 3.33SLIFF400 pKa = 4.02

Molecular weight: 46.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1U7EJ03|A0A1U7EJ03_9VIRU Uncharacterized protein OS=San Bernardo virus OX=1955199 PE=4 SV=1
MM1 pKa = 6.76STNRR5 pKa = 11.84NTNKK9 pKa = 8.47NTKK12 pKa = 8.39QSSGARR18 pKa = 11.84VSAARR23 pKa = 11.84NTTLTKK29 pKa = 10.56AVVRR33 pKa = 11.84NAPKK37 pKa = 9.04RR38 pKa = 11.84TEE40 pKa = 3.95VSSFDD45 pKa = 3.8LNKK48 pKa = 9.99IVSDD52 pKa = 3.83VSSTFLNALNRR63 pKa = 11.84PMVLLSLVMVVALVFTHH80 pKa = 6.66QSDD83 pKa = 4.44FSSGAVGKK91 pKa = 10.0YY92 pKa = 8.94VADD95 pKa = 4.03RR96 pKa = 11.84AEE98 pKa = 4.33TNSLAKK104 pKa = 9.15WVHH107 pKa = 5.23EE108 pKa = 4.17NQTKK112 pKa = 10.21FLGLAIFTPAVLNSPEE128 pKa = 4.39KK129 pKa = 9.38IRR131 pKa = 11.84VVIGLATLLWVMLVPQASVVEE152 pKa = 4.25YY153 pKa = 10.73VLQALALHH161 pKa = 6.63SYY163 pKa = 10.29FRR165 pKa = 11.84VKK167 pKa = 10.26LQHH170 pKa = 5.98SRR172 pKa = 11.84LFIMAMVVVLYY183 pKa = 9.55FMGYY187 pKa = 7.75LTLVKK192 pKa = 10.54SPGSAAGSVNKK203 pKa = 10.17TNDD206 pKa = 2.84RR207 pKa = 3.81

Molecular weight: 22.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

2944

207

2337

981.3

112.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.537 ± 0.86

2.14 ± 0.515

6.046 ± 1.358

5.876 ± 0.768

6.352 ± 1.152

4.178 ± 0.118

2.582 ± 0.149

4.993 ± 0.587

6.352 ± 0.386

8.798 ± 0.57

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.717 ± 0.158

5.197 ± 0.397

3.668 ± 0.337

2.48 ± 0.089

5.74 ± 0.77

8.22 ± 0.383

5.673 ± 0.497

8.865 ± 1.383

0.543 ± 0.09

4.042 ± 0.534
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski