Mycobacterium phage Kumao

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 115 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2D1GPK9|A0A2D1GPK9_9CAUD Scaffolding protein OS=Mycobacterium phage Kumao OX=2041344 GN=16 PE=4 SV=1
MM1 pKa = 7.8ASPYY5 pKa = 11.12AWIIDD10 pKa = 3.74KK11 pKa = 11.08DD12 pKa = 3.89HH13 pKa = 6.82LFEE16 pKa = 6.57PGDD19 pKa = 3.94DD20 pKa = 4.09PSWNDD25 pKa = 3.06KK26 pKa = 9.34GTVGPADD33 pKa = 3.75ANLSDD38 pKa = 3.65EE39 pKa = 4.95AEE41 pKa = 4.57RR42 pKa = 11.84IAQKK46 pKa = 9.99WDD48 pKa = 3.43EE49 pKa = 4.47LGTNYY54 pKa = 9.52EE55 pKa = 4.25HH56 pKa = 6.46QVSFRR61 pKa = 11.84MYY63 pKa = 10.7DD64 pKa = 3.41DD65 pKa = 5.52DD66 pKa = 4.62RR67 pKa = 11.84VLYY70 pKa = 10.73YY71 pKa = 10.85SGTLYY76 pKa = 10.43WNGDD80 pKa = 3.06MRR82 pKa = 11.84EE83 pKa = 4.02EE84 pKa = 3.89YY85 pKa = 10.43AYY87 pKa = 10.65APLGDD92 pKa = 4.03FGMGNAGAVSIEE104 pKa = 4.49YY105 pKa = 9.44IDD107 pKa = 3.88HH108 pKa = 7.06PEE110 pKa = 3.65WDD112 pKa = 3.17ISS114 pKa = 3.58

Molecular weight: 13.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2D1GPY9|A0A2D1GPY9_9CAUD Glutaredoxin OS=Mycobacterium phage Kumao OX=2041344 GN=40 PE=4 SV=1
MM1 pKa = 7.49RR2 pKa = 11.84RR3 pKa = 11.84EE4 pKa = 4.03CPTPYY9 pKa = 10.42KK10 pKa = 9.84RR11 pKa = 11.84TFRR14 pKa = 11.84NMGEE18 pKa = 4.08ALSVRR23 pKa = 11.84QDD25 pKa = 3.09MLRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 10.04KK32 pKa = 9.68GKK34 pKa = 7.12TKK36 pKa = 10.47RR37 pKa = 11.84KK38 pKa = 8.71VEE40 pKa = 4.03SPYY43 pKa = 10.28RR44 pKa = 11.84CCCGGVHH51 pKa = 5.96FTSAPRR57 pKa = 11.84PKK59 pKa = 10.15RR60 pKa = 11.84PRR62 pKa = 11.84FEE64 pKa = 4.39YY65 pKa = 10.42EE66 pKa = 3.78GEE68 pKa = 4.02RR69 pKa = 3.8

Molecular weight: 8.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

115

0

115

21190

23

1996

184.3

20.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.58 ± 0.358

0.963 ± 0.13

6.579 ± 0.204

7.027 ± 0.348

3.072 ± 0.124

8.188 ± 0.417

2.261 ± 0.171

4.79 ± 0.188

4.431 ± 0.248

7.942 ± 0.202

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.223 ± 0.115

3.723 ± 0.191

5.21 ± 0.272

3.714 ± 0.188

6.715 ± 0.372

5.215 ± 0.271

5.389 ± 0.26

7.475 ± 0.25

2.454 ± 0.162

3.049 ± 0.146
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski