Lachnoclostridium sp. An181

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Lachnoclostridium; unclassified Lachnoclostridium

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2355 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y4L023|A0A1Y4L023_9FIRM CRISPR-associated endoribonuclease Cas6 OS=Lachnoclostridium sp. An181 OX=1965575 GN=B5F18_05025 PE=4 SV=1
MM1 pKa = 6.46TQEE4 pKa = 3.87DD5 pKa = 4.94AIAFGDD11 pKa = 3.79GDD13 pKa = 4.04NDD15 pKa = 3.41IEE17 pKa = 4.15MLQAVGLGIAMEE29 pKa = 4.23NASDD33 pKa = 4.32KK34 pKa = 11.12LKK36 pKa = 9.35QTADD40 pKa = 4.04EE41 pKa = 4.47ICGHH45 pKa = 5.25VAGDD49 pKa = 4.0GIYY52 pKa = 10.16YY53 pKa = 9.58YY54 pKa = 10.49CANRR58 pKa = 11.84GLII61 pKa = 3.82

Molecular weight: 6.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y4KW77|A0A1Y4KW77_9FIRM ATP synthase epsilon chain OS=Lachnoclostridium sp. An181 OX=1965575 GN=atpC PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 9.06KK9 pKa = 8.03RR10 pKa = 11.84SRR12 pKa = 11.84SKK14 pKa = 9.44VHH16 pKa = 6.17GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTAGGRR28 pKa = 11.84KK29 pKa = 8.71VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.18GRR39 pKa = 11.84KK40 pKa = 8.18QLSAA44 pKa = 3.9

Molecular weight: 4.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2355

0

2355

821853

27

2505

349.0

39.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.404 ± 0.06

1.325 ± 0.025

5.711 ± 0.054

8.319 ± 0.059

4.068 ± 0.041

7.189 ± 0.043

1.661 ± 0.019

6.999 ± 0.05

7.61 ± 0.044

8.681 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.064 ± 0.027

4.298 ± 0.04

3.111 ± 0.024

3.277 ± 0.028

4.162 ± 0.045

5.544 ± 0.038

5.438 ± 0.049

7.079 ± 0.046

0.984 ± 0.017

4.077 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski