Escherichia phage RB16

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Tevenvirinae; Pseudotevenvirus; Escherichia virus RB16

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 270 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D9ICH8|D9ICH8_9CAUD Conserved hypothetical phage protein OS=Escherichia phage RB16 OX=2681599 GN=RB16p117 PE=4 SV=1
MM1 pKa = 7.76IYY3 pKa = 10.33VSSYY7 pKa = 8.94SADD10 pKa = 3.51YY11 pKa = 10.39EE12 pKa = 4.15FTEE15 pKa = 4.26IVHH18 pKa = 6.01VSEE21 pKa = 5.09SLQNCIDD28 pKa = 3.93ALNKK32 pKa = 9.78VDD34 pKa = 3.83NFPYY38 pKa = 10.6LGDD41 pKa = 4.49NITIDD46 pKa = 2.83VWEE49 pKa = 4.15NEE51 pKa = 3.46KK52 pKa = 10.74RR53 pKa = 11.84IAKK56 pKa = 9.99SSTYY60 pKa = 10.43GYY62 pKa = 10.18RR63 pKa = 11.84DD64 pKa = 3.04VWDD67 pKa = 4.7EE68 pKa = 3.71EE69 pKa = 4.25SQQYY73 pKa = 10.24KK74 pKa = 9.93EE75 pKa = 4.0IPVTFEE81 pKa = 3.99EE82 pKa = 4.5IMANMRR88 pKa = 11.84QVNEE92 pKa = 4.12GG93 pKa = 3.21

Molecular weight: 10.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D9ICW9|D9ICW9_9CAUD Long tail fiber protein p37 OS=Escherichia phage RB16 OX=2681599 GN=36 PE=3 SV=1
MM1 pKa = 7.42FPLKK5 pKa = 10.73ARR7 pKa = 11.84IRR9 pKa = 11.84NKK11 pKa = 10.02TGTQEE16 pKa = 3.4WMEE19 pKa = 4.31CLRR22 pKa = 11.84EE23 pKa = 4.06TKK25 pKa = 9.87HH26 pKa = 6.66YY27 pKa = 10.32YY28 pKa = 9.38ISQAVGYY35 pKa = 9.36DD36 pKa = 3.38GKK38 pKa = 10.6NIRR41 pKa = 11.84AKK43 pKa = 8.27RR44 pKa = 11.84TCWRR48 pKa = 11.84SMIRR52 pKa = 11.84RR53 pKa = 11.84SHH55 pKa = 4.85TPKK58 pKa = 10.65LIKK61 pKa = 9.88IWW63 pKa = 3.52

Molecular weight: 7.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

270

0

270

56230

20

1219

208.3

23.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.875 ± 0.185

1.17 ± 0.061

6.569 ± 0.094

6.824 ± 0.185

4.105 ± 0.107

6.308 ± 0.181

1.839 ± 0.084

6.534 ± 0.119

7.37 ± 0.234

7.539 ± 0.135

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.904 ± 0.07

5.486 ± 0.119

3.594 ± 0.091

3.347 ± 0.092

4.827 ± 0.124

5.867 ± 0.119

5.999 ± 0.243

7.123 ± 0.131

1.352 ± 0.058

4.37 ± 0.099
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski