Escherichia phage P1 (Bacteriophage P1)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Punavirus

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 110 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q71T60|Q71T60_BPP1 C1.100 OS=Escherichia phage P1 OX=10678 GN=c1.100 PE=4 SV=1
MM1 pKa = 7.54AFIPPTIDD9 pKa = 4.44DD10 pKa = 3.84VRR12 pKa = 11.84HH13 pKa = 6.05CSNALSVDD21 pKa = 3.55PAEE24 pKa = 4.25TDD26 pKa = 3.0AARR29 pKa = 11.84AIAEE33 pKa = 4.36HH34 pKa = 6.3YY35 pKa = 10.88SKK37 pKa = 10.62ISNQEE42 pKa = 3.46YY43 pKa = 10.42RR44 pKa = 11.84ITQDD48 pKa = 4.89DD49 pKa = 4.74LDD51 pKa = 5.05DD52 pKa = 4.06LTDD55 pKa = 4.01TIEE58 pKa = 4.42YY59 pKa = 10.89LMATNQPDD67 pKa = 3.65SQQ69 pKa = 3.69

Molecular weight: 7.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q9XJG4|SSB_BPP1 Single-stranded DNA-binding protein OS=Escherichia phage P1 OX=10678 GN=ssb PE=3 SV=1
MM1 pKa = 7.57ASVSISCPSCSATDD15 pKa = 3.7GVVRR19 pKa = 11.84NGKK22 pKa = 8.27STAGHH27 pKa = 4.88QRR29 pKa = 11.84YY30 pKa = 8.94LCSHH34 pKa = 6.46CRR36 pKa = 11.84KK37 pKa = 7.49TWQLQFTYY45 pKa = 9.73TASQPGTHH53 pKa = 5.79QKK55 pKa = 10.33IIDD58 pKa = 3.52MAMNGVGCRR67 pKa = 11.84ATARR71 pKa = 11.84IMGVGLNTIFRR82 pKa = 11.84HH83 pKa = 5.51LKK85 pKa = 8.99NSGRR89 pKa = 11.84SRR91 pKa = 3.37

Molecular weight: 9.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

110

0

110

28126

31

2255

255.7

28.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.269 ± 0.332

1.102 ± 0.127

6.094 ± 0.17

6.638 ± 0.222

3.673 ± 0.166

6.496 ± 0.314

1.792 ± 0.143

6.034 ± 0.135

6.034 ± 0.255

8.714 ± 0.187

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.627 ± 0.117

4.707 ± 0.205

3.883 ± 0.125

4.028 ± 0.145

5.977 ± 0.248

6.659 ± 0.205

5.717 ± 0.281

6.218 ± 0.15

1.262 ± 0.097

3.075 ± 0.158
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski